(Circular) (Six-base) MAPSORT of: pFN21AE1770.seq Check: 1257 from: 1 to: 5536 pFN21AE1770 5536 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2135 2290 2135 Size: 155 5381 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3346 278 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2580 2745 2879 1937 Size: 643 165 134 4594 Fragments arranged by size: 4594 643 165 134 Acc65I G'GTAC_C Cuts at: 2857 2857 Size: 5536 AclI AA'CG_TT Cuts at: 1563 3663 4036 1563 Size: 2100 373 3063 Fragments arranged by size: 3063 2100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3599 4983 5251 1632 Size: 1967 1384 268 1917 Fragments arranged by size: 1967 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5339 AflIII A'CryG_T Cuts at: 1203 2604 4435 5412 1203 Size: 1401 1831 977 1327 Fragments arranged by size: 1831 1401 1327 977 AgeI A'CCGG_T Cuts at: 4367 4367 Size: 5536 AhdI GACnn_n'nnGTC Cuts at: 4265 4265 Size: 5536 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5536 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5536 AlwNI CAG_nnn'CTG Cuts at: 3279 4851 3279 Size: 1572 3964 ApaI G_GGCC'C Cuts at: 2666 2831 2666 Size: 165 5371 ApaLI G'TGCA_C Cuts at: 3593 4749 3593 Size: 1156 4380 ApoI r'AATT_y Cuts at: 1493 2845 3071 3223 5332 1493 Size: 1352 226 152 2109 1697 Fragments arranged by size: 2109 1697 1352 226 152 AseI AT'TA_AT Cuts at: 160 1032 4090 160 Size: 872 3058 1606 Fragments arranged by size: 3058 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 AvaI C'yCGr_G Cuts at: 1870 1943 2861 1870 Size: 73 918 4545 Fragments arranged by size: 4545 918 73 AvrII C'CTAG_G Cuts at: 2260 2275 2260 Size: 15 5521 BamHI G'GATC_C Cuts at: 2775 2866 3233 2775 Size: 91 367 5078 Fragments arranged by size: 5078 367 91 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2465 2492 2857 4312 618 Size: 325 217 627 678 27 365 1455 1842 Fragments arranged by size: 1842 1455 678 627 365 325 217 27 BanII G_rGCy'C Cuts at: 1391 2250 2666 2831 2855 1391 Size: 859 416 165 24 4072 Fragments arranged by size: 4072 859 416 165 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2064 928 Size: 971 165 4400 Fragments arranged by size: 4400 971 165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3727 3761 1903 Size: 34 1790 34 3678 Fragments arranged by size: 3678 1790 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3428 4638 3428 Size: 1210 4326 BclI T'GATC_A Cuts at: 1548 1548 Size: 5536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4147 136 Size: 107 122 71 631 605 2475 1525 Fragments arranged by size: 2475 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5531 5531 Size: 5536 BlpI GC'TnA_GC Cuts at: 2953 2953 Size: 5536 Bme1580I G_kGCm'C Cuts at: 1790 2495 2666 2831 3597 4753 1790 Size: 705 171 165 766 1156 2573 Fragments arranged by size: 2573 1156 766 705 171 165 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5536 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3267 4225 442 Size: 416 53 83 399 24 1850 958 1753 Fragments arranged by size: 1850 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5536 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 5536 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 4196 1239 Size: 1169 1788 2579 Fragments arranged by size: 2579 1788 1169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2743 3661 4526 4824 2743 Size: 918 865 298 3455 Fragments arranged by size: 3455 918 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4199 882 Size: 617 2700 2219 Fragments arranged by size: 2700 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3327 493 Size: 711 2123 2702 Fragments arranged by size: 2702 2123 711 BsaBI GATnn'nnATC Cuts at: 5530 5530 Size: 5536 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3343 3725 4360 275 Size: 53 83 186 936 1810 382 635 1451 Fragments arranged by size: 1810 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2340 3969 4367 4641 4788 5199 2340 Size: 1629 398 274 147 411 2677 Fragments arranged by size: 2677 1629 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2184 2214 2381 2411 1178 Size: 30 165 30 781 30 167 30 4303 Fragments arranged by size: 4303 781 167 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2579 1186 Size: 1393 4143 BseYI C'CCAG_C Cuts at: 2487 2805 4739 5235 2487 Size: 318 1934 496 2788 Fragments arranged by size: 2788 1934 496 318 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4963 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2986 3747 4775 1540 Size: 275 186 985 761 1028 2301 Fragments arranged by size: 2301 1028 985 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3597 3682 4753 3597 Size: 85 1071 4380 Fragments arranged by size: 4380 1071 85 BsmI GAATG_Cn' Cuts at: 3047 3140 3047 Size: 93 5443 BsmBI CGTCTCn'nnnn_ Cuts at: 2704 2704 Size: 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2495 2666 2831 2855 3597 3682 Size: 399 460 245 171 165 24 742 85 Cuts at: 3682 4753 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 460 399 245 171 165 85 24 BspEI T'CCGG_A Cuts at: 5199 5199 Size: 5536 BspHI T'CATG_A Cuts at: 3425 5155 3425 Size: 1730 3806 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2135 2290 2891 844 Size: 1291 155 601 3489 Fragments arranged by size: 3489 1291 601 155 BsrBI CCG'CTC Cuts at: 1394 1823 3423 1394 Size: 429 1600 3507 Fragments arranged by size: 3507 1600 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4031 4205 65 Size: 1183 2783 174 1396 Fragments arranged by size: 2783 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2615 2733 4180 4367 1700 Size: 110 805 118 1447 187 2869 Fragments arranged by size: 2869 1447 805 187 118 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5536 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5536 BssSI C'ACGA_G Cuts at: 3596 4608 3596 Size: 1012 4524 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5536 BstBI TT'CG_AA Cuts at: 4340 4340 Size: 5536 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5536 BstYI r'GATC_y Cuts at: 1972 2775 2866 3233 3618 3635 5087 5173 5185 Size: 803 91 367 385 17 1452 86 12 Cuts at: 5185 5531 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 803 385 367 346 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4984 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4797 Fragments arranged by size: 4797 683 56 BtsI GCAGTG_nn' Cuts at: 2220 3052 3846 3866 2220 Size: 832 794 20 3890 Fragments arranged by size: 3890 832 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5536 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5501 DraI TTT'AAA Cuts at: 2840 2840 Size: 5536 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4543 809 Size: 507 3227 1802 Fragments arranged by size: 3227 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2983 3872 5501 Size: 54 1425 50 275 18 1153 889 1629 Cuts at: 5501 8 Size: 43 Fragments arranged by size: 1629 1425 1153 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2983 1537 Size: 275 1171 4090 Fragments arranged by size: 4090 1171 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3466 1353 Size: 2113 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4123 4497 4643 387 Size: 1064 138 2534 374 146 1280 Fragments arranged by size: 2534 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 3599 4196 4983 5251 1239 Size: 393 776 1191 597 787 268 1524 Fragments arranged by size: 1524 1191 787 776 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2215 2662 2663 2827 2215 Size: 447 1 164 4924 Fragments arranged by size: 4924 447 164 1 EcoRI G'AATT_C Cuts at: 2845 3223 2845 Size: 378 5158 FspI TGC'GCA Cuts at: 4042 4042 Size: 5536 HaeII r_GCGC'y Cuts at: 1678 4683 1678 Size: 3005 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2184 2216 2381 2413 4177 Size: 32 135 32 612 32 165 32 1764 Cuts at: 4177 4209 4251 4283 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1764 612 165 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2880 3126 669 Size: 988 281 942 246 3079 Fragments arranged by size: 3079 988 942 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5536 HpaI GTT'AAC Cuts at: 3126 3126 Size: 5536 KpnI G_GTAC'C Cuts at: 2861 2861 Size: 5536 MfeI C'AATT_G Cuts at: 3135 3135 Size: 5536 MluI A'CGCG_T Cuts at: 5412 5412 Size: 5536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2572 4650 4834 1305 Size: 1267 2078 184 2007 Fragments arranged by size: 2078 2007 1267 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3714 Fragments arranged by size: 3714 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2370 3494 3853 4012 518 Size: 648 1204 1124 359 159 2042 Fragments arranged by size: 2042 1204 1124 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2952 3629 4777 5022 1606 Size: 1346 677 1148 245 2120 Fragments arranged by size: 2120 1346 1148 677 245 NaeI GCC'GGC Cuts at: 2617 2617 Size: 5536 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4984 NdeI CA'TA_TG Cuts at: 387 387 Size: 5536 NgoMIV G'CCGG_C Cuts at: 2615 2615 Size: 5536 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5536 NotI GC'GGCC_GC Cuts at: 2983 2983 Size: 5536 NspI r_CATG'y Cuts at: 1132 1229 2608 2894 4439 1132 Size: 97 1379 286 1545 2229 Fragments arranged by size: 2229 1545 1379 286 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5536 PciI A'CATG_T Cuts at: 2604 4435 2604 Size: 1831 3705 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5536 PmeI GTTT'AAAC Cuts at: 2840 2840 Size: 5536 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3605 3637 5118 5150 3605 Size: 32 1481 32 3991 Fragments arranged by size: 3991 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2215 2215 Size: 5536 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5536 PsiI TTA'TAA Cuts at: 3106 3106 Size: 5536 PspOMI G'GGCC_C Cuts at: 2662 2827 2662 Size: 165 5371 PstI C_TGCA'G Cuts at: 830 2397 2888 830 Size: 1567 491 3478 Fragments arranged by size: 3478 1567 491 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5536 SalI G'TCGA_C Cuts at: 1936 2878 1936 Size: 942 4594 SbfI CC_TGCA'GG Cuts at: 2888 2888 Size: 5536 ScaI AGT'ACT Cuts at: 3784 5496 3784 Size: 1712 3824 SfcI C'TryA_G Cuts at: 826 1045 2393 2884 4019 4700 4891 826 Size: 219 1348 491 1135 681 191 1471 Fragments arranged by size: 1471 1348 1135 681 491 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5536 SmaI CCC'GGG Cuts at: 1872 2863 1872 Size: 991 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2758 3640 4541 4803 820 Size: 197 926 815 882 901 262 1553 Fragments arranged by size: 1553 926 901 882 815 262 197 SnaBI TAC'GTA Cuts at: 493 3327 493 Size: 2834 2702 SpeI A'CTAG_T Cuts at: 152 2164 152 Size: 2012 3524 SphI G_CATG'C Cuts at: 1132 2894 1132 Size: 1762 3774 SspI AAT'ATT Cuts at: 5 52 3460 5 Size: 47 3408 2081 Fragments arranged by size: 3408 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5536 StyI C'CwwG_G Cuts at: 513 1065 2040 2076 2260 2275 2975 513 Size: 552 975 36 184 15 700 3074 Fragments arranged by size: 3074 975 700 552 184 36 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2369 3563 3580 3733 3918 4363 325 Size: 1255 789 1194 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1194 789 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3782 5494 96 Size: 274 80 33 51 1443 1805 1712 138 Fragments arranged by size: 1805 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5496 5528 5496 Size: 32 5504 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5536 XbaI T'CTAG_A Cuts at: 2872 2872 Size: 5536 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5536 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5536 XmaI C'CCGG_G Cuts at: 1870 2861 1870 Size: 991 4545 XmnI GAAnn'nnTTC Cuts at: 3665 3665 Size: 5536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3344 276 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI