################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 694 GTCTAC GT^[AC][GT]AC AccII 128 CGCG CG^CG AluI 421 AGCT AG^CT AlwNI 263 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 612 GGGCCC GGGCC^C AspHI 508 GAGCAC G[AT]GC[AT]^C BanI 415 GGCACC G^G[CT][GA]CC BanII 612 GGGCCC G[GA]GC[CT]^C BbsI 123 GAAGAC GAAGAC(2/6) BpmI 229 CTCCAG CTGGAG(16/14) BpuAI 123 GAAGAC GAAGAC(2/6) BsaI 55 GAGACC GGTCTC(1/5) BsgI 571 GTGCAG GTGCAG(16/14) BsiHKAI 508 GAGCAC G[AT]GC[AT]^C BsmBI 733 GAGACG CGTCTC(1/5) BsrDI 323 GCAATG GCAATG(2/0) BstAPI 416 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 128 CGCG CG^CG CfoI 488 GCGC GCG^C DdeI 269 CTGAG C^T[ACGT]AG DraIII 398 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 554 CCATGG C^C[GA][CT]GG EarI 635 CTCTTC CTCTTC(1/4) EcoNI 228 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 554 CCATGG C^C[AT][AT]GG FauI 197 CCCGC CCCGC(4/6) HhaI 488 GCGC GCG^C HinP1I 488 GCGC G^CGC Ksp632I 635 CTCTTC CTCTTC(1/4) MaeII 448 ACGT A^CGT MseI 16 TTAA T^TAA MspA1I 420 CAGCTG C[AC]G^C[GT]G MvnI 128 CGCG CG^CG NcoI 554 CCATGG C^CATGG PflMI 362 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 10 CTGCAG CTGCA^G PvuII 420 CAGCTG CAG^CTG SfcI 10 CTGCAG C^T[GA][CT]AG SmlI 190 CTCAAG C^T[CT][GA]AG StyI 554 CCATGG C^C[AT][AT]GG TfiI 6 GATTC G^A[AT]TC Tru9I 16 TTAA T^TAA Van91I 362 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BamHI 663 GGATCC G^GATCC 725 GGATCC BmyI 508 GAGCAC G[AGT]GC[ACT]^C 612 GGGCCC BseRI 393 CTCCTC GAGGAG(10/8) 238 GAGGAG Bsp1286I 508 GAGCAC G[AGT]GC[ACT]^C 612 GGGCCC BstYI 663 GGATCC [GA]^GATC[CT] 725 GGATCC DraII 611 GGGGCCC [GA]G^G[ACGT]CC[CT] 612 GGGCCCT EcoO109I 611 GGGGCCC [GA]G^G[ACGT]CC[CT] 612 GGGCCCT HaeIII 613 GGCC GG^CC 658 GGCC HgaI 126 GACGC GACGC(5/10) 147 GACGC MflI 663 GGATCC [GA]^GATC[CT] 725 GGATCC NlaIII 333 CATG CATG^ 555 CATG SfaNI 485 GATGC GCATC(5/9) 349 GCATC TaqI 77 TCGA T^CGA 119 TCGA Tsp45I 406 GTCAC ^GT[GC]AC 602 GTGAC TthHB8I 77 TCGA T^CGA 119 TCGA XhoII 663 GGATCC [GA]^GATC[CT] 725 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AlwI BbvI BcnI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI TseI TspRI