(Circular) (Six-base) MAPSORT of: pFN21AE6258.seq Check: 7115 from: 1 to: 5455 pFN21AE6258 5455 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3265 278 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2700 2798 1937 Size: 763 98 4594 Fragments arranged by size: 4594 763 98 Acc65I G'GTAC_C Cuts at: 2776 2776 Size: 5455 AclI AA'CG_TT Cuts at: 1563 3582 3955 1563 Size: 2019 373 3063 Fragments arranged by size: 3063 2019 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3518 4902 5170 1632 Size: 1886 1384 268 1917 Fragments arranged by size: 1917 1886 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5258 AflIII A'CryG_T Cuts at: 1203 4354 5331 1203 Size: 3151 977 1327 Fragments arranged by size: 3151 1327 977 AgeI A'CCGG_T Cuts at: 4286 4286 Size: 5455 AhdI GACnn_n'nnGTC Cuts at: 4184 4184 Size: 5455 AleI CACnn'nnGTG Cuts at: 1166 2561 1166 Size: 1395 4060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2206 1184 Size: 1022 4433 AlwNI CAG_nnn'CTG Cuts at: 2273 3198 4770 2273 Size: 925 1572 2958 Fragments arranged by size: 2958 1572 925 ApaI G_GGCC'C Cuts at: 2621 2621 Size: 5455 ApaLI G'TGCA_C Cuts at: 3512 4668 3512 Size: 1156 4299 ApoI r'AATT_y Cuts at: 1493 2764 2990 3142 5251 1493 Size: 1271 226 152 2109 1697 Fragments arranged by size: 2109 1697 1271 226 152 AseI AT'TA_AT Cuts at: 160 1032 4009 160 Size: 872 2977 1606 Fragments arranged by size: 2977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 AvaI C'yCGr_G Cuts at: 1870 1943 2780 1870 Size: 73 837 4545 Fragments arranged by size: 4545 837 73 BamHI G'GATC_C Cuts at: 2668 2730 2785 3152 2668 Size: 62 55 367 4971 Fragments arranged by size: 4971 367 62 55 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2420 2776 4231 618 Size: 325 217 627 633 356 1455 1842 Fragments arranged by size: 1842 1455 633 627 356 325 217 BanII G_rGCy'C Cuts at: 1391 2621 2774 1391 Size: 1230 153 4072 Fragments arranged by size: 4072 1230 153 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2135 928 Size: 971 236 4248 Fragments arranged by size: 4248 971 236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3646 3680 1903 Size: 34 1709 34 3678 Fragments arranged by size: 3678 1709 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 4557 3347 Size: 1210 4245 BclI T'GATC_A Cuts at: 1548 1548 Size: 5455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4066 136 Size: 107 122 71 631 605 2394 1525 Fragments arranged by size: 2394 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5450 5450 Size: 5455 BlpI GC'TnA_GC Cuts at: 2872 2872 Size: 5455 Bme1580I G_kGCm'C Cuts at: 1790 2621 3516 4672 1790 Size: 831 895 1156 2573 Fragments arranged by size: 2573 1156 895 831 BmgBI CAC'GTC Cuts at: 1318 2454 1318 Size: 1136 4319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2458 3186 4144 Size: 416 53 83 399 24 1041 728 958 Cuts at: 4144 442 Size: 1753 Fragments arranged by size: 1753 1041 958 728 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5455 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 4115 1239 Size: 980 1896 2579 Fragments arranged by size: 2579 1896 980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2180 3580 4445 4743 2180 Size: 1400 865 298 2892 Fragments arranged by size: 2892 1400 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2054 4118 882 Size: 617 555 2064 2219 Fragments arranged by size: 2219 2064 617 555 BsaAI yAC'GTr Cuts at: 493 1204 3246 493 Size: 711 2042 2702 Fragments arranged by size: 2702 2042 711 BsaBI GATnn'nnATC Cuts at: 5449 5449 Size: 5455 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3262 3644 4279 275 Size: 53 83 186 936 1729 382 635 1451 Fragments arranged by size: 1729 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3888 4286 4560 4707 5118 3888 Size: 398 274 147 411 4225 Fragments arranged by size: 4225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5230 Fragments arranged by size: 5230 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 2389 1186 Size: 1072 131 4252 Fragments arranged by size: 4252 1072 131 BseYI C'CCAG_C Cuts at: 2100 2442 4658 5154 2100 Size: 342 2216 496 2401 Fragments arranged by size: 2401 2216 496 342 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2597 1163 Size: 573 861 4021 Fragments arranged by size: 4021 861 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2905 3666 4694 1540 Size: 275 186 904 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2517 3516 3601 4672 2517 Size: 999 85 1071 3300 Fragments arranged by size: 3300 1071 999 85 BsmI GAATG_Cn' Cuts at: 2966 3059 2966 Size: 93 5362 BsmBI CGTCTCn'nnnn_ Cuts at: 2732 2732 Size: 5455 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2517 2621 2774 3516 3601 4672 1391 Size: 399 727 104 153 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 727 399 153 104 85 BspEI T'CCGG_A Cuts at: 5118 5118 Size: 5455 BspHI T'CATG_A Cuts at: 3344 5074 3344 Size: 1730 3725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2810 844 Size: 1966 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3342 1394 Size: 429 1519 3507 Fragments arranged by size: 3507 1519 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2335 3950 4124 65 Size: 1183 1087 1615 174 1396 Fragments arranged by size: 1615 1396 1183 1087 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4099 4286 1700 Size: 110 2289 187 2869 Fragments arranged by size: 2869 2289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5455 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5455 BssSI C'ACGA_G Cuts at: 3515 4527 3515 Size: 1012 4443 BstAPI GCAn_nnn'nTGC Cuts at: 794 2427 794 Size: 1633 3822 BstBI TT'CG_AA Cuts at: 4259 4259 Size: 5455 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5455 BstYI r'GATC_y Cuts at: 1972 2668 2730 2785 3152 3537 3554 5006 5092 Size: 696 62 55 367 385 17 1452 86 Cuts at: 5092 5104 5450 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 696 385 367 346 86 62 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2559 513 Size: 552 1494 3409 Fragments arranged by size: 3409 1494 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2143 2340 2503 487 Size: 683 56 917 197 163 3439 Fragments arranged by size: 3439 917 683 197 163 56 BtsI GCAGTG_nn' Cuts at: 2188 2412 2971 3765 3785 2188 Size: 224 559 794 20 3858 Fragments arranged by size: 3858 794 559 224 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5420 DraI TTT'AAA Cuts at: 2759 2759 Size: 5455 DraIII CAC_nnn'GTG Cuts at: 2408 2408 Size: 5455 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4462 809 Size: 507 3146 1802 Fragments arranged by size: 3146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2902 3791 5420 Size: 54 1425 50 275 18 1072 889 1629 Cuts at: 5420 8 Size: 43 Fragments arranged by size: 1629 1425 1072 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2902 1537 Size: 275 1090 4090 Fragments arranged by size: 4090 1090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2646 3385 1353 Size: 1293 739 3423 Fragments arranged by size: 3423 1293 739 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4042 4416 4562 387 Size: 1064 138 2453 374 146 1280 Fragments arranged by size: 2453 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 3518 4115 4902 5170 1239 Size: 393 587 1299 597 787 268 1524 Fragments arranged by size: 1524 1299 787 597 587 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2237 2237 Size: 5455 EcoO109I rG'GnC_Cy Cuts at: 2617 2618 2617 Size: 1 5454 EcoRI G'AATT_C Cuts at: 2764 3142 2764 Size: 378 5077 FspI TGC'GCA Cuts at: 3961 3961 Size: 5455 HaeII r_GCGC'y Cuts at: 1678 4602 1678 Size: 2924 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4096 4128 4170 4202 1373 Size: 32 135 32 2524 32 42 32 2626 Fragments arranged by size: 2626 2524 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2799 3045 669 Size: 988 281 861 246 3079 Fragments arranged by size: 3079 988 861 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5455 HpaI GTT'AAC Cuts at: 3045 3045 Size: 5455 KpnI G_GTAC'C Cuts at: 2780 2780 Size: 5455 MfeI C'AATT_G Cuts at: 3054 3054 Size: 5455 MluI A'CGCG_T Cuts at: 5331 5331 Size: 5455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4569 4753 1305 Size: 3264 184 2007 Fragments arranged by size: 3264 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3633 Fragments arranged by size: 3633 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 2342 2561 3413 3772 3931 518 Size: 648 1162 14 219 852 359 159 2042 Fragments arranged by size: 2042 1162 852 648 359 219 159 14 MspA1I CmG'CkG Cuts at: 1606 2427 2871 3548 4696 4941 1606 Size: 821 444 677 1148 245 2120 Fragments arranged by size: 2120 1148 821 677 444 245 NcoI C'CATG_G Cuts at: 513 1065 2559 513 Size: 552 1494 3409 Fragments arranged by size: 3409 1494 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5455 NotI GC'GGCC_GC Cuts at: 2902 2902 Size: 5455 NspI r_CATG'y Cuts at: 1132 1229 2813 4358 1132 Size: 97 1584 1545 2229 Fragments arranged by size: 2229 1584 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5455 PciI A'CATG_T Cuts at: 4354 4354 Size: 5455 PflMI CCAn_nnn'nTGG Cuts at: 1108 2373 1108 Size: 1265 4190 PfoI T'CCnGG_A Cuts at: 2733 2733 Size: 5455 PmeI GTTT'AAAC Cuts at: 2759 2759 Size: 5455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3524 3556 5037 5069 3524 Size: 32 1481 32 3910 Fragments arranged by size: 3910 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5455 PsiI TTA'TAA Cuts at: 3025 3025 Size: 5455 PspOMI G'GGCC_C Cuts at: 2617 2617 Size: 5455 PstI C_TGCA'G Cuts at: 830 2019 2807 830 Size: 1189 788 3478 Fragments arranged by size: 3478 1189 788 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5455 PvuII CAG'CTG Cuts at: 2427 2427 Size: 5455 SalI G'TCGA_C Cuts at: 1936 2797 1936 Size: 861 4594 SbfI CC_TGCA'GG Cuts at: 2807 2807 Size: 5455 ScaI AGT'ACT Cuts at: 3703 5415 3703 Size: 1712 3743 SfcI C'TryA_G Cuts at: 826 1045 2015 2803 3938 4619 4810 826 Size: 219 970 788 1135 681 191 1471 Fragments arranged by size: 1471 1135 970 788 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5455 SmaI CCC'GGG Cuts at: 1872 2782 1872 Size: 910 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2195 3559 4460 4722 820 Size: 197 926 252 1364 901 262 1553 Fragments arranged by size: 1553 1364 926 901 262 252 197 SnaBI TAC'GTA Cuts at: 493 3246 493 Size: 2753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5455 SphI G_CATG'C Cuts at: 1132 2813 1132 Size: 1681 3774 SspI AAT'ATT Cuts at: 5 52 3379 5 Size: 47 3327 2081 Fragments arranged by size: 3327 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5455 StyI C'CwwG_G Cuts at: 513 1065 2559 2894 513 Size: 552 1494 335 3074 Fragments arranged by size: 3074 1494 552 335 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3482 3499 3652 3837 4282 325 Size: 1255 1902 17 153 185 445 1498 Fragments arranged by size: 1902 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3701 5413 96 Size: 274 80 33 51 1443 1724 1712 138 Fragments arranged by size: 1724 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5415 5447 5415 Size: 32 5423 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5455 XbaI T'CTAG_A Cuts at: 2791 2791 Size: 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5455 XmaI C'CCGG_G Cuts at: 1870 2780 1870 Size: 910 4545 XmnI GAAnn'nnTTC Cuts at: 3584 3584 Size: 5455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3263 276 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI