(Linear) (Six-base) MAPSORT of: KIEE6266.seq Check: 291 from: 1 to: 762 KIEE6266.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AccI GT'mk_AC Cuts at: 0 707 762 Size: 707 55 Acc65I G'GTAC_C Cuts at: 0 38 762 Size: 38 724 AflIII A'CryG_T Cuts at: 0 506 762 Size: 506 256 ApaI G_GGCC'C Cuts at: 0 629 762 Size: 629 133 ApoI r'AATT_y Cuts at: 0 724 762 Size: 724 38 AvaI C'yCGr_G Cuts at: 0 168 762 Size: 168 594 BanI G'GyrC_C Cuts at: 0 38 311 762 Size: 38 273 451 Fragments arranged by size: 451 273 38 BanII G_rGCy'C Cuts at: 0 629 762 Size: 629 133 BglI GCCn_nnn'nGGC Cuts at: 0 692 762 Size: 692 70 Bme1580I G_kGCm'C Cuts at: 0 424 629 762 Size: 424 205 133 Fragments arranged by size: 424 205 133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 762 Size: 277 485 BsaHI Gr'CG_yC Cuts at: 0 173 762 Size: 173 589 BsaWI w'CCGG_w Cuts at: 0 395 762 Size: 395 367 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 538 762 Size: 538 224 BseYI C'CCAG_C Cuts at: 0 240 455 562 762 Size: 240 215 107 200 Fragments arranged by size: 240 215 200 107 BsiWI C'GTAC_G Cuts at: 0 306 762 Size: 306 456 BsmI GAATG_Cn' Cuts at: 0 485 762 Size: 485 277 BsmBI CGTCTCn'nnnn_ Cuts at: 0 75 762 Size: 75 687 Bsp1286I G_dGCh'C Cuts at: 0 424 629 762 Size: 424 205 133 Fragments arranged by size: 424 205 133 BspEI T'CCGG_A Cuts at: 0 395 762 Size: 395 367 BsrDI GCAATG_nn' Cuts at: 0 407 762 Size: 407 355 BsrFI r'CCGG_y Cuts at: 0 577 762 Size: 577 185 BstAPI GCAn_nnn'nTGC Cuts at: 0 195 572 762 Size: 195 377 190 Fragments arranged by size: 377 195 190 BstXI CCAn_nnnn'nTGG Cuts at: 0 240 762 Size: 240 522 BstYI r'GATC_y Cuts at: 0 61 762 Size: 61 701 BtgI C'CryG_G Cuts at: 0 428 762 Size: 428 334 BtsI GCAGTG_nn' Cuts at: 0 196 424 502 762 Size: 196 228 78 260 Fragments arranged by size: 260 228 196 78 DraIII CAC_nnn'GTG Cuts at: 0 734 762 Size: 734 28 EaeI y'GGCC_r Cuts at: 0 2 684 762 Size: 2 682 78 Fragments arranged by size: 682 78 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 596 762 Size: 596 166 EcoO109I rG'GnC_Cy Cuts at: 0 625 626 654 762 Size: 625 1 28 108 Fragments arranged by size: 625 108 28 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 53 85 320 352 762 Size: 53 32 235 32 410 Fragments arranged by size: 410 235 53 32 32 KpnI G_GTAC'C Cuts at: 0 42 762 Size: 42 720 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 344 762 Size: 344 418 MscI TGG'CCA Cuts at: 0 4 686 762 Size: 4 682 76 Fragments arranged by size: 682 76 4 MspA1I CmG'CkG Cuts at: 0 430 552 762 Size: 430 122 210 Fragments arranged by size: 430 210 122 NaeI GCC'GGC Cuts at: 0 579 762 Size: 579 183 NgoMIV G'CCGG_C Cuts at: 0 577 762 Size: 577 185 NspI r_CATG'y Cuts at: 0 570 762 Size: 570 192 PasI CC'CwG_GG Cuts at: 0 20 762 Size: 20 742 PflMI CCAn_nnn'nTGG Cuts at: 0 288 762 Size: 288 474 PspOMI G'GGCC_C Cuts at: 0 625 762 Size: 625 137 PstI C_TGCA'G Cuts at: 0 85 112 497 535 762 Size: 85 27 385 38 227 Fragments arranged by size: 385 227 85 38 27 SacII CC_GC'GG Cuts at: 0 431 762 Size: 431 331 SfcI C'TryA_G Cuts at: 0 81 108 493 531 762 Size: 81 27 385 38 231 Fragments arranged by size: 385 231 81 38 27 SgrAI Cr'CCGG_yG Cuts at: 0 577 762 Size: 577 185 SmlI C'TyrA_G Cuts at: 0 256 762 Size: 256 506 StyI C'CwwG_G Cuts at: 0 48 762 Size: 48 714 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 183 329 762 Size: 183 146 433 Fragments arranged by size: 433 183 146 TatI w'GTAC_w Cuts at: 0 320 762 Size: 320 442 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 200 232 762 Size: 200 32 530 Fragments arranged by size: 530 200 32 Enzymes that do cut: AccI Acc65I AflIII ApaI ApoI AvaI BanI BanII BglI Bme1580I BpuEI BsaHI BsaWI BseRI BseYI BsiWI BsmI BsmBI Bsp1286I BspEI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI DraIII EaeI EciI EcoO109I Hin4I KpnI MmeI MscI MspA1I NaeI NgoMIV NspI PasI PflMI PspOMI PstI SacII SfcI SgrAI SmlI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaXI BsgI BsiEI BsiHKAI BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MslI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI