Restriction map for
pFN21AE2039
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII BclI BfrI BglII
BsaBI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 127 GACGTC GACGT^C
Acc65I 974 GGTACC G^GTACC
AcsI 1507 GAATTC [GA]^AATT[CT]
Aor51HI 58 AGCGCT AGC^GCT
ApoI 1507 GAATTC [GA]^AATT[CT]
Asp718I 974 GGTACC G^GTACC
AvrII 969 CCTAGG C^CTAGG
BalI 521 TGGCCA TGG^CCA
BamHI 651 GGATCC G^GATCC
BbeI 1933 GGCGCC GGCGC^C
BbrPI 865 CACGTG CAC^GTG
BbsI 556 GTCTTC GAAGAC(2/6)
BciVI 1108 GGATAC GTATCC(6/5)
BfaI 970 CTAG C^TAG
BlnI 969 CCTAGG C^CTAGG
BlpI 1683 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1683 GCTGAGC GC^T[ACGT]AGC
BpuAI 556 GTCTTC GAAGAC(2/6)
BsaAI 865 CACGTG [CT]AC^GT[GA]
BsaI 1369 GAGACC GGTCTC(1/5)
BsrFI 10 GCCGGC [GA]^CCGG[CT]
BstEII 429 GGTGACC G^GT[ACGT]ACC
BstPI 429 GGTGACC G^GT[ACGT]ACC
CelII 1683 GCTGAGC GC^T[ACGT]AGC
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
DraIII 1756 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 58 AGCGCT AGC^GCT
EcoO65I 429 GGTGACC G^GT[ACGT]ACC
EcoRI 1507 GAATTC G^AATTC
EcoRV 1204 GATATC GAT^ATC
EcoT14I 969 CCTAGG C^C[AT][AT]GG
HgaI 497 GCGTC GACGC(5/10)
KasI 1933 GGCGCC G^GCGCC
KpnI 974 GGTACC GGTAC^C
KspI 471 CCGCGG CCGC^GG
MaeI 970 CTAG C^TAG
MluNI 521 TGGCCA TGG^CCA
MscI 521 TGGCCA TGG^CCA
MslI 1617 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 10 GCCGGC GCC^GGC
NarI 1933 GGCGCC GG^CGCC
NgoMIV 10 GCCGGC G^CCGGC
NspI 1223 GCATGT [GA]CATG^[CT]
PmaCI 865 CACGTG CAC^GTG
PmlI 865 CACGTG CAC^GTG
SacI 1336 GAGCTC GAGCT^C
SacII 471 CCGCGG CCGC^GG
SexAI 419 ACCTGGT A^CC[AT]GGT
SfaNI 1306 GATGC GCATC(5/9)
SfoI 1933 GGCGCC GGC^GCC
SmlI 1654 CTCAAG C^T[CT][GA]AG
StyI 969 CCTAGG C^C[AT][AT]GG
Tsp509I 1508 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 127 GACGTC G[GA]^CG[CT]C
1933 GGCGCC
AfaI 45 GTAC GT^AC
975 GTAC
AocI 724 CCTGAGG CC^T[ACGT]AGG
1407 CCTGAGG
AspHI 1003 GAGCAC G[AT]GC[AT]^C
1336 GAGCTC
BglI 335 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
863 GCCACGTGGGC
BsaHI 127 GACGTC G[GA]^CG[CT]C
1933 GGCGCC
BsaWI 911 ACCGGA [AT]^CCGG[AT]
1478 TCCGGT
BsiHKAI 1003 GAGCAC G[AT]GC[AT]^C
1336 GAGCTC
BsmAI 80 GAGAC GTCTC(1/5)
1369 GAGAC
BssSI 312 CACGAG CACGAG(-5/-1)
1339 CTCGTG
Bsu36I 724 CCTGAGG CC^T[ACGT]AGG
1407 CCTGAGG
DrdI 121 GACAATGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
262 GACTGGGCTGTC
EarI 181 CTCTTC CTCTTC(1/4)
1357 GAAGAG
Eco81I 724 CCTGAGG CC^T[ACGT]AGG
1407 CCTGAGG
EcoNI 885 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1911 CCTGCCCGAGG
HaeII 58 AGCGCT [GA]GCGC^[CT]
1933 GGCGCC
Hin1I 127 GACGTC G[GA]^CG[CT]C
1933 GGCGCC
Ksp632I 181 CTCTTC CTCTTC(1/4)
1357 GAAGAG
PpuMI 893 AGGTCCC [GA]G^G[AT]CC[CT]
1170 GGGTCCC
RsaI 45 GTAC GT^AC
975 GTAC
SmaI 1182 CCCGGG CCC^GGG
1844 CCCGGG
StuI 402 AGGCCT AGG^CCT
953 AGGCCT
XcmI 400 CCAGGCCTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1595 CCATCCTGGACATGG
XmaCI 1182 CCCGGG C^CCGGG
1844 CCCGGG
XmaI 1182 CCCGGG C^CCGGG
1844 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaI AvaII BanI BanII BbvCI
BbvI BcgI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
Sau3AI Sau96I ScrFI SfcI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII