(Circular) (Six-base) MAPSORT of: pFN21AE2039.seq Check: 261 from: 1 to: 6649 pFN21AE2039 6649 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2613 3591 2613 Size: 978 5671 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2136 4459 278 Size: 53 83 186 936 600 2323 2468 Fragments arranged by size: 2468 2323 936 600 186 83 53 AccI GT'mk_AC Cuts at: 1937 3992 1937 Size: 2055 4594 Acc65I G'GTAC_C Cuts at: 2979 3970 2979 Size: 991 5658 AclI AA'CG_TT Cuts at: 1563 4776 5149 1563 Size: 3213 373 3063 Fragments arranged by size: 3213 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2659 2901 2930 4712 6096 6364 1632 Size: 1027 242 29 1782 1384 268 1917 Fragments arranged by size: 1917 1782 1384 1027 268 242 29 AfeI AGC'GCT Cuts at: 2065 2065 Size: 6649 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6452 AflIII A'CryG_T Cuts at: 1203 5548 6525 1203 Size: 4345 977 1327 Fragments arranged by size: 4345 1327 977 AgeI A'CCGG_T Cuts at: 5480 5480 Size: 6649 AhdI GACnn_n'nnGTC Cuts at: 5378 5378 Size: 6649 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6649 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3074 1184 Size: 1890 4759 AlwNI CAG_nnn'CTG Cuts at: 4392 5964 4392 Size: 1572 5077 ApaI G_GGCC'C Cuts at: 2840 2879 3856 2840 Size: 39 977 5633 Fragments arranged by size: 5633 977 39 ApaLI G'TGCA_C Cuts at: 4706 5862 4706 Size: 1156 5493 ApoI r'AATT_y Cuts at: 1493 3512 3958 4184 4336 6445 1493 Size: 2019 446 226 152 2109 1697 Fragments arranged by size: 2109 2019 1697 446 226 152 AseI AT'TA_AT Cuts at: 160 1032 5203 160 Size: 872 4171 1606 Fragments arranged by size: 4171 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 AvaI C'yCGr_G Cuts at: 1870 1943 2688 2774 2926 3179 3187 3392 3749 Size: 73 745 86 152 253 8 205 357 Cuts at: 3749 3849 3920 3974 1870 Size: 100 71 54 4545 Fragments arranged by size: 4545 745 357 253 205 152 100 86 73 71 54 8 AvrII C'CTAG_G Cuts at: 2974 2974 Size: 6649 BamHI G'GATC_C Cuts at: 2656 3979 4346 2656 Size: 1323 367 4959 Fragments arranged by size: 4959 1323 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2483 2711 2979 3938 3970 Size: 325 217 627 696 228 268 959 32 Cuts at: 3970 5425 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 959 696 627 325 268 228 217 32 BanII G_rGCy'C Cuts at: 1391 2152 2316 2334 2351 2775 2840 2879 2942 Size: 761 164 18 17 424 65 39 63 Cuts at: 2942 3080 3345 3564 3804 3856 3968 1391 Size: 138 265 219 240 52 112 4072 Fragments arranged by size: 4072 761 424 265 240 219 164 138 112 65 63 52 39 18 17 BbeI G_GCGC'C Cuts at: 3942 3942 Size: 6649 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2554 928 Size: 971 655 5023 Fragments arranged by size: 5023 971 655 BbvCI CC'TCA_GC Cuts at: 2411 2507 2984 3003 2411 Size: 96 477 19 6057 Fragments arranged by size: 6057 477 96 19 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2561 2595 3109 3143 3293 3327 4840 Size: 34 624 34 514 34 150 34 1513 Cuts at: 4840 4874 1903 Size: 34 3678 Fragments arranged by size: 3678 1513 624 514 150 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3107 4541 5751 3107 Size: 1434 1210 4005 Fragments arranged by size: 4005 1434 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6649 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2346 2874 5260 Size: 107 122 71 631 605 674 528 2386 Cuts at: 5260 136 Size: 1525 Fragments arranged by size: 2386 1525 674 631 605 528 122 107 71 BglII A'GATC_T Cuts at: 6644 6644 Size: 6649 BlpI GC'TnA_GC Cuts at: 3689 4066 3689 Size: 377 6272 Bme1580I G_kGCm'C Cuts at: 1790 2486 2716 2840 2866 2879 3856 4710 5866 Size: 696 230 124 26 13 977 854 1156 Cuts at: 5866 1790 Size: 2573 Fragments arranged by size: 2573 1156 977 854 696 230 124 26 13 BmgBI CAC'GTC Cuts at: 1318 3703 1318 Size: 2385 4264 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2155 2278 4380 Size: 416 53 83 399 24 738 123 2102 Cuts at: 4380 5338 442 Size: 958 1753 Fragments arranged by size: 2102 1753 958 738 416 399 123 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6649 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2108 Size: 6649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2187 2188 2466 2636 2826 3929 5309 1239 Size: 948 1 278 170 190 1103 1380 2579 Fragments arranged by size: 2579 1380 1103 948 278 190 170 1 Bpu10I CC'TnA_GC Cuts at: 2411 2507 2904 2984 3003 3081 2411 Size: 96 397 80 19 78 5979 Fragments arranged by size: 5979 397 96 80 78 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3644 4774 5639 5937 3644 Size: 1130 865 298 4356 Fragments arranged by size: 4356 1130 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3368 5312 882 Size: 617 1869 1944 2219 Fragments arranged by size: 2219 1944 1869 617 BsaAI yAC'GTr Cuts at: 493 1204 2872 4440 493 Size: 711 1668 1568 2702 Fragments arranged by size: 2702 1668 1568 711 BsaBI GATnn'nnATC Cuts at: 6643 6643 Size: 6649 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 3939 4456 4838 Size: 53 83 186 936 600 1806 517 382 Cuts at: 4838 5473 275 Size: 635 1451 Fragments arranged by size: 1806 1451 936 635 600 517 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2916 3483 5082 5480 5754 5901 6312 2916 Size: 567 1599 398 274 147 411 3253 Fragments arranged by size: 3253 1599 567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2369 2399 2634 2664 3536 Size: 30 165 30 966 30 235 30 872 Cuts at: 3536 3566 1178 Size: 30 4261 Fragments arranged by size: 4261 966 872 235 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2246 2566 2867 2870 3020 3206 3938 Size: 988 72 320 301 3 150 186 732 Cuts at: 3938 3941 1186 Size: 3 3894 Fragments arranged by size: 3894 988 732 320 301 186 150 72 3 3 BseYI C'CCAG_C Cuts at: 2333 2415 2809 2942 2988 3193 5852 6348 2333 Size: 82 394 133 46 205 2659 496 2634 Fragments arranged by size: 2659 2634 496 394 205 133 82 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2039 2126 2346 2450 3563 3710 3788 Size: 573 303 87 220 104 1113 147 78 Cuts at: 3788 1163 Size: 4024 Fragments arranged by size: 4024 1113 573 303 220 147 104 87 78 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4099 4860 5888 1540 Size: 275 186 2098 761 1028 2301 Fragments arranged by size: 2301 2098 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3012 3345 4710 4795 5866 3012 Size: 333 1365 85 1071 3795 Fragments arranged by size: 3795 1365 1071 333 85 BsmI GAATG_Cn' Cuts at: 4160 4253 4160 Size: 93 6556 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2316 2334 2351 2486 2716 2775 Size: 399 362 164 18 17 135 230 59 Cuts at: 2775 2840 2866 2879 2942 3012 3080 3345 3564 Size: 65 26 13 63 70 68 265 219 Cuts at: 3564 3804 3856 3968 4710 4795 5866 1391 Size: 240 52 112 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 362 265 240 230 219 164 135 112 85 70 68 65 63 59 52 26 18 17 13 BspEI T'CCGG_A Cuts at: 6312 6312 Size: 6649 BspHI T'CATG_A Cuts at: 4538 6268 4538 Size: 1730 4919 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2061 2526 2613 2887 3591 4004 844 Size: 1217 465 87 274 704 413 3489 Fragments arranged by size: 3489 1217 704 465 413 274 87 BsrBI CCG'CTC Cuts at: 1394 1823 2397 2833 3091 4536 1394 Size: 429 574 436 258 1445 3507 Fragments arranged by size: 3507 1445 574 436 429 258 BsrDI GCAATG_nn' Cuts at: 65 1248 5144 5318 65 Size: 1183 3896 174 1396 Fragments arranged by size: 3896 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 5293 5480 1700 Size: 110 205 3278 187 2869 Fragments arranged by size: 3278 2869 205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6649 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6649 BssSI C'ACGA_G Cuts at: 2317 3344 4709 5721 2317 Size: 1027 1365 1012 3245 Fragments arranged by size: 3245 1365 1027 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6649 BstBI TT'CG_AA Cuts at: 5453 5453 Size: 6649 BstEII G'GTnAC_C Cuts at: 1184 2434 1184 Size: 1250 5399 BstYI r'GATC_y Cuts at: 1972 2656 2919 3654 3774 3912 3979 4346 4731 Size: 684 263 735 120 138 67 367 385 Cuts at: 4731 4748 6200 6286 6298 6644 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 735 684 385 367 346 263 138 120 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2730 3413 2730 Size: 683 5966 BtgI C'CryG_G Cuts at: 513 1065 2004 2476 2826 3876 513 Size: 552 939 472 350 1050 3286 Fragments arranged by size: 3286 1050 939 552 472 350 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2080 487 Size: 683 56 854 5056 Fragments arranged by size: 5056 854 683 56 BtsI GCAGTG_nn' Cuts at: 2462 3068 4165 4959 4979 2462 Size: 606 1097 794 20 4132 Fragments arranged by size: 4132 1097 794 606 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6649 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6614 DraI TTT'AAA Cuts at: 3953 3953 Size: 6649 DraIII CAC_nnn'GTG Cuts at: 3766 3766 Size: 6649 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2132 2273 5656 809 Size: 507 816 141 3383 1802 Fragments arranged by size: 3383 1802 816 507 141 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2291 2526 2819 Size: 54 1425 50 275 18 461 235 293 Cuts at: 2819 4096 4985 6614 8 Size: 1277 889 1629 43 Fragments arranged by size: 1629 1425 1277 889 461 293 275 235 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4096 1537 Size: 275 2284 4090 Fragments arranged by size: 4090 2284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2192 3357 4579 1353 Size: 839 1165 1222 3423 Fragments arranged by size: 3423 1222 1165 839 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2058 2381 5236 5610 5756 387 Size: 1064 138 469 323 2855 374 146 1280 Fragments arranged by size: 2855 1280 1064 469 374 323 146 138 EcoICRI GAG'CTC Cuts at: 3343 3343 Size: 6649 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2188 2466 2636 2659 2826 2901 Size: 393 555 1 278 170 23 167 75 Cuts at: 2901 2930 3929 4712 5309 6096 6364 1239 Size: 29 999 783 597 787 268 1524 Fragments arranged by size: 1524 999 787 783 597 555 393 278 268 170 167 75 29 23 1 EcoNI CCTnn'n_nnAGG Cuts at: 2894 3920 2894 Size: 1026 5623 EcoO109I rG'GnC_Cy Cuts at: 2813 2836 2837 2876 2899 3176 3721 3853 2813 Size: 23 1 39 23 277 545 132 5609 Fragments arranged by size: 5609 545 277 132 39 23 23 1 EcoRI G'AATT_C Cuts at: 3512 3958 4336 3512 Size: 446 378 5825 Fragments arranged by size: 5825 446 378 EcoRV GAT'ATC Cuts at: 3211 3211 Size: 6649 FspI TGC'GCA Cuts at: 5155 5155 Size: 6649 HaeII r_GCGC'y Cuts at: 1678 2067 3942 5796 1678 Size: 389 1875 1854 2531 Fragments arranged by size: 2531 1875 1854 389 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 2369 2401 2648 Size: 32 135 32 500 32 265 32 247 Cuts at: 2648 2680 2775 2807 3260 3292 3449 3481 5290 Size: 32 95 32 453 32 157 32 1809 Cuts at: 5290 5322 5364 5396 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1809 500 453 265 247 157 135 95 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3993 4239 669 Size: 988 281 2055 246 3079 Fragments arranged by size: 3079 2055 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6649 HpaI GTT'AAC Cuts at: 4239 4239 Size: 6649 KasI G'GCGC_C Cuts at: 3938 3938 Size: 6649 KpnI G_GTAC'C Cuts at: 2983 3974 2983 Size: 991 5658 MfeI C'AATT_G Cuts at: 4248 4248 Size: 6649 MluI A'CGCG_T Cuts at: 6525 6525 Size: 6649 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2613 2769 5763 5947 1305 Size: 1308 156 2994 184 2007 Fragments arranged by size: 2994 2007 1308 184 156 MscI TGG'CCA Cuts at: 10 64 1489 1832 2528 10 Size: 54 1425 343 696 4131 Fragments arranged by size: 4131 1425 696 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3626 4607 4966 5125 518 Size: 648 2460 981 359 159 2042 Fragments arranged by size: 2460 2042 981 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2337 2415 2478 2988 3002 3832 4065 4742 Size: 731 78 63 510 14 830 233 677 Cuts at: 4742 5890 6135 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 830 731 677 510 245 233 78 63 14 NaeI GCC'GGC Cuts at: 2017 2017 Size: 6649 NarI GG'CG_CC Cuts at: 3939 3939 Size: 6649 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5158 Fragments arranged by size: 5158 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6649 NgoMIV G'CCGG_C Cuts at: 2015 2015 Size: 6649 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6649 NotI GC'GGCC_GC Cuts at: 4096 4096 Size: 6649 NspI r_CATG'y Cuts at: 1132 1229 3232 4007 5552 1132 Size: 97 2003 775 1545 2229 Fragments arranged by size: 2229 2003 1545 775 97 PasI CC'CwG_GG Cuts at: 1348 2344 1348 Size: 996 5653 PciI A'CATG_T Cuts at: 5548 5548 Size: 6649 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6649 PfoI T'CCnGG_A Cuts at: 2652 3444 3522 3603 2652 Size: 792 78 81 5698 Fragments arranged by size: 5698 792 81 78 PmeI GTTT'AAAC Cuts at: 3953 3953 Size: 6649 PmlI CAC'GTG Cuts at: 2872 2872 Size: 6649 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4718 4750 6231 6263 4718 Size: 32 1481 32 5104 Fragments arranged by size: 5104 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2899 3176 2899 Size: 277 6372 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6649 PsiI TTA'TAA Cuts at: 4219 4219 Size: 6649 PspOMI G'GGCC_C Cuts at: 2836 2875 3852 2836 Size: 39 977 5633 Fragments arranged by size: 5633 977 39 PstI C_TGCA'G Cuts at: 830 2610 2721 2895 3063 4001 830 Size: 1780 111 174 168 938 3478 Fragments arranged by size: 3478 1780 938 174 168 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6649 PvuII CAG'CTG Cuts at: 2337 2415 2988 3002 2337 Size: 78 573 14 5984 Fragments arranged by size: 5984 573 78 14 SacI G_AGCT'C Cuts at: 3345 3345 Size: 6649 SacII CC_GC'GG Cuts at: 2479 2479 Size: 6649 SalI G'TCGA_C Cuts at: 1936 3991 1936 Size: 2055 4594 SanDI GG'GwC_CC Cuts at: 3176 3176 Size: 6649 SbfI CC_TGCA'GG Cuts at: 4001 4001 Size: 6649 ScaI AGT'ACT Cuts at: 4897 6609 4897 Size: 1712 4937 SexAI A'CCwGG_T Cuts at: 2424 2424 Size: 6649 SfcI C'TryA_G Cuts at: 826 1045 2020 2154 2606 2717 2891 3059 3997 Size: 219 975 134 452 111 174 168 938 Cuts at: 3997 5132 5813 6004 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 975 938 681 452 219 191 174 168 134 111 SfoI GGC'GCC Cuts at: 3940 3940 Size: 6649 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6649 SmaI CCC'GGG Cuts at: 1872 3189 3851 3976 1872 Size: 1317 662 125 4545 Fragments arranged by size: 4545 1317 662 125 SmlI C'TyrA_G Cuts at: 820 1017 1943 3659 4753 5654 5916 820 Size: 197 926 1716 1094 901 262 1553 Fragments arranged by size: 1716 1553 1094 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4440 493 Size: 3947 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6649 SphI G_CATG'C Cuts at: 1132 4007 1132 Size: 2875 3774 SspI AAT'ATT Cuts at: 5 52 4573 5 Size: 47 4521 2081 Fragments arranged by size: 4521 2081 47 StuI AGG'CCT Cuts at: 1517 2409 2960 1517 Size: 892 551 5206 Fragments arranged by size: 5206 892 551 StyI C'CwwG_G Cuts at: 513 1065 2004 2974 4088 513 Size: 552 939 970 1114 3074 Fragments arranged by size: 3074 1114 970 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2097 2192 4676 4693 4846 5031 5476 Size: 1255 517 95 2484 17 153 185 445 Cuts at: 5476 325 Size: 1498 Fragments arranged by size: 2484 1498 1255 517 445 185 153 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4895 6607 96 Size: 274 80 33 51 1443 2918 1712 138 Fragments arranged by size: 2918 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2454 2486 2634 2666 3536 3568 6609 6641 2454 Size: 32 148 32 870 32 3041 32 2462 Fragments arranged by size: 3041 2462 870 148 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6649 XbaI T'CTAG_A Cuts at: 3985 3985 Size: 6649 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2412 3607 2412 Size: 1195 5454 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6649 XmaI C'CCGG_G Cuts at: 1870 3187 3849 3974 1870 Size: 1317 662 125 4545 Fragments arranged by size: 4545 1317 662 125 XmnI GAAnn'nnTTC Cuts at: 4778 4778 Size: 6649 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2134 4457 276 Size: 53 83 186 936 600 2323 2468 Fragments arranged by size: 2468 2323 936 600 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BsmBI BstXI BstZ17I FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SapI SfiI SrfI SwaI