(Linear) (Six-base) MAPSORT of: KIEE2039.seq Check: 583 from: 1 to: 1944 KIEE2039.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 608 1586 1944 Size: 608 978 358 Fragments arranged by size: 978 608 358 AatII G_ACGT'C Cuts at: 0 131 1944 Size: 131 1813 Acc65I G'GTAC_C Cuts at: 0 974 1944 Size: 974 970 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 654 896 925 1944 Size: 654 242 29 1019 Fragments arranged by size: 1019 654 242 29 AfeI AGC'GCT Cuts at: 0 60 1944 Size: 60 1884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1069 1944 Size: 1069 875 ApaI G_GGCC'C Cuts at: 0 835 874 1851 1944 Size: 835 39 977 93 Fragments arranged by size: 977 835 93 39 ApoI r'AATT_y Cuts at: 0 1507 1944 Size: 1507 437 AvaI C'yCGr_G Cuts at: 0 683 769 921 1174 1182 1387 1744 1844 Size: 683 86 152 253 8 205 357 100 Cuts at: 1844 1915 1944 Size: 71 29 Fragments arranged by size: 683 357 253 205 152 100 86 71 29 8 AvrII C'CTAG_G Cuts at: 0 969 1944 Size: 969 975 BamHI G'GATC_C Cuts at: 0 651 1944 Size: 651 1293 BanI G'GyrC_C Cuts at: 0 478 706 974 1933 1944 Size: 478 228 268 959 11 Fragments arranged by size: 959 478 268 228 11 BanII G_rGCy'C Cuts at: 0 147 311 329 346 770 835 874 937 Size: 147 164 18 17 424 65 39 63 Cuts at: 937 1075 1340 1559 1799 1851 1944 Size: 138 265 219 240 52 93 Fragments arranged by size: 424 265 240 219 164 147 138 93 65 63 52 39 18 17 BbeI G_GCGC'C Cuts at: 0 1937 1944 Size: 1937 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 549 1944 Size: 549 1395 BbvCI CC'TCA_GC Cuts at: 0 406 502 979 998 1944 Size: 406 96 477 19 946 Fragments arranged by size: 946 477 406 96 19 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 556 590 1104 1138 1288 1322 1944 Size: 556 34 514 34 150 34 622 Fragments arranged by size: 622 556 514 150 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1102 1944 Size: 1102 842 BglI GCCn_nnn'nGGC Cuts at: 0 341 869 1944 Size: 341 528 1075 Fragments arranged by size: 1075 528 341 BlpI GC'TnA_GC Cuts at: 0 1684 1944 Size: 1684 260 Bme1580I G_kGCm'C Cuts at: 0 481 711 835 861 874 1851 1944 Size: 481 230 124 26 13 977 93 Fragments arranged by size: 977 481 230 124 93 26 13 BmgBI CAC'GTC Cuts at: 0 1698 1944 Size: 1698 246 BmrI ACTGGGnnnn_n' Cuts at: 0 150 273 1944 Size: 150 123 1671 Fragments arranged by size: 1671 150 123 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 103 1944 Size: 103 1841 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 183 461 631 821 1924 1944 Size: 182 1 278 170 190 1103 20 Fragments arranged by size: 1103 278 190 182 170 20 1 Bpu10I CC'TnA_GC Cuts at: 0 406 502 899 979 998 1076 1944 Size: 406 96 397 80 19 78 868 Fragments arranged by size: 868 406 397 96 80 78 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1639 1944 Size: 1639 305 BsaI GGTCTCn'nnnn_ Cuts at: 0 1363 1944 Size: 1363 581 BsaAI yAC'GTr Cuts at: 0 867 1944 Size: 867 1077 BsaHI Gr'CG_yC Cuts at: 0 128 1934 1944 Size: 128 1806 10 Fragments arranged by size: 1806 128 10 BsaWI w'CCGG_w Cuts at: 0 911 1478 1944 Size: 911 567 466 Fragments arranged by size: 911 567 466 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 364 394 629 659 1531 1561 1944 Size: 364 30 235 30 872 30 383 Fragments arranged by size: 872 383 364 235 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 169 241 561 862 865 1015 1201 1933 Size: 169 72 320 301 3 150 186 732 Cuts at: 1933 1936 1944 Size: 3 8 Fragments arranged by size: 732 320 301 186 169 150 72 8 3 3 BseYI C'CCAG_C Cuts at: 0 328 410 804 937 983 1188 1944 Size: 328 82 394 133 46 205 756 Fragments arranged by size: 756 394 328 205 133 82 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 121 341 445 1558 1705 1783 1944 Size: 34 87 220 104 1113 147 78 161 Fragments arranged by size: 1113 220 161 147 104 87 78 34 BsiHKAI G_wGCw'C Cuts at: 0 1007 1340 1944 Size: 1007 333 604 Fragments arranged by size: 1007 604 333 Bsp1286I G_dGCh'C Cuts at: 0 147 311 329 346 481 711 770 835 Size: 147 164 18 17 135 230 59 65 Cuts at: 835 861 874 937 1007 1075 1340 1559 1799 Size: 26 13 63 70 68 265 219 240 Cuts at: 1799 1851 1944 Size: 52 93 Fragments arranged by size: 265 240 230 219 164 147 135 93 70 68 65 63 59 52 26 18 17 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 56 521 608 882 1586 1944 Size: 56 465 87 274 704 358 Fragments arranged by size: 704 465 358 274 87 56 BsrBI CCG'CTC Cuts at: 0 392 828 1086 1944 Size: 392 436 258 858 Fragments arranged by size: 858 436 392 258 BsrFI r'CCGG_y Cuts at: 0 10 1944 Size: 10 1934 BssSI C'ACGA_G Cuts at: 0 312 1339 1944 Size: 312 1027 605 Fragments arranged by size: 1027 605 312 BstEII G'GTnAC_C Cuts at: 0 429 1944 Size: 429 1515 BstYI r'GATC_y Cuts at: 0 651 914 1649 1769 1907 1944 Size: 651 263 735 120 138 37 Fragments arranged by size: 735 651 263 138 120 37 Bsu36I CC'TnA_GG Cuts at: 0 725 1408 1944 Size: 725 683 536 Fragments arranged by size: 725 683 536 BtgI C'CryG_G Cuts at: 0 471 821 1871 1944 Size: 471 350 1050 73 Fragments arranged by size: 1050 471 350 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 75 1944 Size: 75 1869 BtsI GCAGTG_nn' Cuts at: 0 457 1063 1944 Size: 457 606 881 Fragments arranged by size: 881 606 457 DraIII CAC_nnn'GTG Cuts at: 0 1761 1944 Size: 1761 183 DrdI GACnn_nn'nnGTC Cuts at: 0 127 268 1944 Size: 127 141 1676 Fragments arranged by size: 1676 141 127 EaeI y'GGCC_r Cuts at: 0 286 521 814 1944 Size: 286 235 293 1130 Fragments arranged by size: 1130 293 286 235 EarI CTCTTCn'nnn_ Cuts at: 0 187 1352 1944 Size: 187 1165 592 Fragments arranged by size: 1165 592 187 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 53 376 1944 Size: 53 323 1568 Fragments arranged by size: 1568 323 53 EcoICRI GAG'CTC Cuts at: 0 1338 1944 Size: 1338 606 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 183 461 631 654 821 896 925 Size: 182 1 278 170 23 167 75 29 Cuts at: 925 1924 1944 Size: 999 20 Fragments arranged by size: 999 278 182 170 167 75 29 23 20 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 889 1915 1944 Size: 889 1026 29 Fragments arranged by size: 1026 889 29 EcoO109I rG'GnC_Cy Cuts at: 0 808 831 832 871 894 1171 1716 1848 Size: 808 23 1 39 23 277 545 132 Cuts at: 1848 1944 Size: 96 Fragments arranged by size: 808 545 277 132 96 39 23 23 1 EcoRI G'AATT_C Cuts at: 0 1507 1944 Size: 1507 437 EcoRV GAT'ATC Cuts at: 0 1206 1944 Size: 1206 738 HaeII r_GCGC'y Cuts at: 0 62 1937 1944 Size: 62 1875 7 Fragments arranged by size: 1875 62 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 67 99 364 396 643 675 770 802 Size: 67 32 265 32 247 32 95 32 Cuts at: 802 1255 1287 1444 1476 1944 Size: 453 32 157 32 468 Fragments arranged by size: 468 453 265 247 157 95 67 32 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1933 1944 Size: 1933 11 KpnI G_GTAC'C Cuts at: 0 978 1944 Size: 978 966 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 608 764 1944 Size: 608 156 1180 Fragments arranged by size: 1180 608 156 MscI TGG'CCA Cuts at: 0 523 1944 Size: 523 1421 MslI CAynn'nnrTG Cuts at: 0 1621 1944 Size: 1621 323 MspA1I CmG'CkG Cuts at: 0 332 410 473 983 997 1827 1944 Size: 332 78 63 510 14 830 117 Fragments arranged by size: 830 510 332 117 78 63 14 NaeI GCC'GGC Cuts at: 0 12 1944 Size: 12 1932 NarI GG'CG_CC Cuts at: 0 1934 1944 Size: 1934 10 NgoMIV G'CCGG_C Cuts at: 0 10 1944 Size: 10 1934 NspI r_CATG'y Cuts at: 0 1227 1944 Size: 1227 717 PasI CC'CwG_GG Cuts at: 0 339 1944 Size: 339 1605 PfoI T'CCnGG_A Cuts at: 0 647 1439 1517 1598 1944 Size: 647 792 78 81 346 Fragments arranged by size: 792 647 346 81 78 PmlI CAC'GTG Cuts at: 0 867 1944 Size: 867 1077 PpuMI rG'GwC_Cy Cuts at: 0 894 1171 1944 Size: 894 277 773 Fragments arranged by size: 894 773 277 PspOMI G'GGCC_C Cuts at: 0 831 870 1847 1944 Size: 831 39 977 97 Fragments arranged by size: 977 831 97 39 PstI C_TGCA'G Cuts at: 0 605 716 890 1058 1944 Size: 605 111 174 168 886 Fragments arranged by size: 886 605 174 168 111 PvuII CAG'CTG Cuts at: 0 332 410 983 997 1944 Size: 332 78 573 14 947 Fragments arranged by size: 947 573 332 78 14 SacI G_AGCT'C Cuts at: 0 1340 1944 Size: 1340 604 SacII CC_GC'GG Cuts at: 0 474 1944 Size: 474 1470 SanDI GG'GwC_CC Cuts at: 0 1171 1944 Size: 1171 773 SexAI A'CCwGG_T Cuts at: 0 419 1944 Size: 419 1525 SfcI C'TryA_G Cuts at: 0 15 149 601 712 886 1054 1944 Size: 15 134 452 111 174 168 890 Fragments arranged by size: 890 452 174 168 134 111 15 SfoI GGC'GCC Cuts at: 0 1935 1944 Size: 1935 9 SmaI CCC'GGG Cuts at: 0 1184 1846 1944 Size: 1184 662 98 Fragments arranged by size: 1184 662 98 SmlI C'TyrA_G Cuts at: 0 1654 1944 Size: 1654 290 StuI AGG'CCT Cuts at: 0 404 955 1944 Size: 404 551 989 Fragments arranged by size: 989 551 404 StyI C'CwwG_G Cuts at: 0 969 1944 Size: 969 975 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 92 187 1944 Size: 92 95 1757 Fragments arranged by size: 1757 95 92 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 449 481 629 661 1531 1563 1944 Size: 449 32 148 32 870 32 381 Fragments arranged by size: 870 449 381 148 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 407 1602 1944 Size: 407 1195 342 Fragments arranged by size: 1195 407 342 XmaI C'CCGG_G Cuts at: 0 1182 1844 1944 Size: 1182 662 100 Fragments arranged by size: 1182 662 100 ZraI GAC'GTC Cuts at: 0 129 1944 Size: 129 1815 Enzymes that do cut: AarI AatII Acc65I AcuI AfeI AlfI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TstI XcmI XmaI ZraI Enzymes that do not cut: AccI AclI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI BaeI BclI BfrBI BglII BmtI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I ClaI CspCI DraI EagI FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI