Restriction map for
pFN21AE1820
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BbeI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MslI MunI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfoI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 181 GTAC GT^AC
AocI 312 CCTTAGG CC^T[ACGT]AGG
AvaI 799 CCCGGG C^[CT]CG[GA]G
BamHI 932 GGATCC G^GATCC
BbrPI 910 CACGTG CAC^GTG
BbvCI 291 GCTGAGG CCTCAGC(-5/-2)
BlpI 855 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 855 GCTGAGC GC^T[ACGT]AGC
BsaAI 910 CACGTG [CT]AC^GT[GA]
BsoBI 799 CCCGGG C^[CT]CG[GA]G
BsrI 142 CCAGT ACTGG(1/-1)
BstBI 401 TTCGAA TT^CGAA
BstYI 932 GGATCC [GA]^GATC[CT]
Bsu36I 312 CCTTAGG CC^T[ACGT]AGG
CelII 855 GCTGAGC GC^T[ACGT]AGC
DpnII 933 GATC ^GATC
Eco81I 312 CCTTAGG CC^T[ACGT]AGG
HgaI 72 GCGTC GACGC(5/10)
MaeII 911 ACGT A^CGT
MaeIII 74 GTCAC ^GT[ACGT]AC
MboI 933 GATC ^GATC
MflI 932 GGATCC [GA]^GATC[CT]
MseI 671 TTAA T^TAA
NaeI 860 GCCGGC GCC^GGC
NdeII 933 GATC ^GATC
NgoMIV 860 GCCGGC G^CCGGC
NspV 401 TTCGAA TT^CGAA
PmaCI 910 CACGTG CAC^GTG
PmlI 910 CACGTG CAC^GTG
RsaI 181 GTAC GT^AC
Sau3AI 933 GATC ^GATC
SexAI 240 ACCAGGT A^CC[AT]GGT
SfaNI 959 GCATC GCATC(5/9)
SfcI 759 CTGTAG C^T[GA][CT]AG
SfiI 831 GGCCCGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 401 TTCGAA TT^CGAA
SmaI 799 CCCGGG CCC^GGG
Tru9I 671 TTAA T^TAA
Tsp45I 74 GTCAC ^GT[GC]AC
XcmI 55 CCAGAGGCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 932 GGATCC [GA]^GATC[CT]
XmaCI 799 CCCGGG C^CCGGG
XmaI 799 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 154 CGCG CG^CG
865 CGCG
Alw44I 92 GTGCAC G^TGCAC
913 GTGCAC
AlwI 933 GATCC GGATC(4/5)
932 GGATC
ApaI 231 GGGCCC GGGCC^C
802 GGGCCC
ApaLI 92 GTGCAC G^TGCAC
913 GTGCAC
AviII 146 TGCGCA TGC^GCA
701 TGCGCA
BanI 244 GGTGCC G^G[CT][GA]CC
883 GGCACC
BbsI 283 GAAGAC GAAGAC(2/6)
410 GTCTTC
BpmI 512 CTCCAG CTGGAG(16/14)
649 CTGGAG
BpuAI 283 GAAGAC GAAGAC(2/6)
410 GTCTTC
BsaI 198 GAGACC GGTCTC(1/5)
335 GGTCTC
BsmAI 198 GAGAC GTCTC(1/5)
336 GTCTC
BsrFI 124 GCCGGT [GA]^CCGG[CT]
860 GCCGGC
BstUI 154 CGCG CG^CG
865 CGCG
Cfr10I 124 GCCGGT [GA]^CCGG[CT]
860 GCCGGC
EarI 265 GAAGAG CTCTTC(1/4)
373 GAAGAG
EcoNI 308 CCTTCCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
645 CCTGCTGGAGG
EcoT14I 251 CCATGG C^C[AT][AT]GG
848 CCATGG
FspI 146 TGCGCA TGC^GCA
701 TGCGCA
HphI 322 GGTGA GGTGA(8/7)
973 TCACC
Ksp632I 265 GAAGAG CTCTTC(1/4)
373 GAAGAG
MvnI 154 CGCG CG^CG
865 CGCG
NcoI 251 CCATGG C^CATGG
848 CCATGG
PflMI 537 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
675 CCAGAAGCTGG
PpuMI 296 GGGTCCT [GA]G^G[AT]CC[CT]
597 GGGTCCC
PvuII 550 CAGCTG CAG^CTG
565 CAGCTG
SapI 265 GAAGAGC GCTCTTC(1/4)
373 GAAGAGC
SphI 61 GCATGC GCATG^C
793 GCATGC
StyI 251 CCATGG C^C[AT][AT]GG
848 CCATGG
Tsp509I 84 AATT ^AATT
590 AATT
TspRI 143 CAGTG CA[GC]TG[ACGT][ACGT]^
190 CAGTG
Van91I 537 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
675 CCAGAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BanII BbvI BcnI BfaI BglI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeI MboII MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
NspI PleI Sau96I ScrFI TaqI
TseI TthHB8I