(Circular) (Six-base) MAPSORT of: pFN21AE1820.seq Check: 4858 from: 1 to: 5707 pFN21AE1820 5707 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2289 3770 5154 5422 1632 Size: 657 1481 1384 268 1917 Fragments arranged by size: 1917 1481 1384 657 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2544 2576 2544 Size: 32 5675 AlwNI CAG_nnn'CTG Cuts at: 2267 2548 2686 3450 5022 2267 Size: 281 138 764 1572 2952 Fragments arranged by size: 2952 1572 764 281 138 ApaI G_GGCC'C Cuts at: 2240 2811 2240 Size: 571 5136 ApaLI G'TGCA_C Cuts at: 2097 2918 3764 4920 2097 Size: 821 846 1156 2884 Fragments arranged by size: 2884 1156 846 821 ApoI r'AATT_y Cuts at: 1493 3016 3242 3394 5503 1493 Size: 1523 226 152 2109 1697 Fragments arranged by size: 2109 1697 1523 226 152 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2804 3032 1870 Size: 73 861 228 4545 Fragments arranged by size: 4545 861 228 73 BamHI G'GATC_C Cuts at: 2937 3037 3404 2937 Size: 100 367 5240 Fragments arranged by size: 5240 367 100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2888 3028 4483 618 Size: 325 217 627 462 639 140 1455 1842 Fragments arranged by size: 1842 1455 639 627 462 325 217 140 BanII G_rGCy'C Cuts at: 1391 2240 2283 2811 2832 3026 1391 Size: 849 43 528 21 194 4072 Fragments arranged by size: 4072 849 528 194 43 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2295 2408 928 Size: 971 396 113 4227 Fragments arranged by size: 4227 971 396 113 BbvCI CC'TCA_GC Cuts at: 2297 2297 Size: 5707 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2020 2258 2843 Size: 107 122 71 631 605 348 238 585 Cuts at: 2843 4318 136 Size: 1475 1525 Fragments arranged by size: 1525 1475 631 605 585 348 238 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 2861 3124 2861 Size: 263 5444 Bme1580I G_kGCm'C Cuts at: 1790 2101 2240 2811 2891 2922 3768 4924 1790 Size: 311 139 571 80 31 846 1156 2573 Fragments arranged by size: 2573 1156 846 571 311 139 80 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2141 3438 4396 Size: 416 53 83 399 24 724 1297 958 Cuts at: 4396 442 Size: 1753 Fragments arranged by size: 1753 1297 958 724 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2502 2675 4367 1239 Size: 1263 173 1692 2579 Fragments arranged by size: 2579 1692 1263 173 Bpu10I CC'TnA_GC Cuts at: 2297 2396 2297 Size: 99 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4697 4995 3832 Size: 865 298 4544 Fragments arranged by size: 4544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2197 2346 4370 882 Size: 617 698 149 2024 2219 Fragments arranged by size: 2219 2024 698 617 149 BsaAI yAC'GTr Cuts at: 493 1204 2917 3498 493 Size: 711 1713 581 2702 Fragments arranged by size: 2702 1713 711 581 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3514 3896 4531 275 Size: 53 83 186 936 1981 382 635 1451 Fragments arranged by size: 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5482 Fragments arranged by size: 5482 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2215 2236 2451 2672 2675 1186 Size: 1029 21 215 221 3 4218 Fragments arranged by size: 4218 1029 221 215 21 3 BseYI C'CCAG_C Cuts at: 2018 2070 2102 2124 2275 2509 2541 2585 2881 Size: 52 32 22 151 234 32 44 296 Cuts at: 2881 2955 4910 5406 2018 Size: 74 1955 496 2319 Fragments arranged by size: 2319 1955 496 296 234 151 74 52 44 32 32 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2101 2916 2922 3768 3853 4924 2101 Size: 815 6 846 85 1071 2884 Fragments arranged by size: 2884 1071 846 815 85 6 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2240 2283 2811 2832 2891 2916 Size: 399 311 139 43 528 21 59 25 Cuts at: 2916 2922 3026 3768 3853 4924 1391 Size: 6 104 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 528 399 311 139 104 85 59 43 25 21 6 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 844 Size: 2218 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4202 4376 65 Size: 1183 2954 174 1396 Fragments arranged by size: 2954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 2865 4351 4538 1700 Size: 110 319 736 1486 187 2869 Fragments arranged by size: 2869 1486 736 319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 2407 4511 2407 Size: 2104 3603 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstYI r'GATC_y Cuts at: 1972 2937 3037 3404 3789 3806 5258 5344 5356 Size: 965 100 367 385 17 1452 86 12 Cuts at: 5356 5702 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 965 385 367 346 100 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2318 2318 Size: 5707 BtgI C'CryG_G Cuts at: 513 1065 2004 2256 2853 2947 2982 513 Size: 552 939 252 597 94 35 3238 Fragments arranged by size: 3238 939 597 552 252 94 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 3223 4017 4037 3223 Size: 794 20 4893 Fragments arranged by size: 4893 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2198 2233 529 Size: 35 1634 35 4003 Fragments arranged by size: 4003 1634 35 35 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2057 2874 2895 Size: 54 1425 50 275 18 227 817 21 Cuts at: 2895 3154 4043 5672 8 Size: 259 889 1629 43 Fragments arranged by size: 1629 1425 889 817 275 259 227 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2265 2373 3637 1353 Size: 912 108 1264 3423 Fragments arranged by size: 3423 1264 912 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2100 2471 2571 2711 4294 4668 Size: 1064 138 511 371 100 140 1583 374 Cuts at: 4668 4814 387 Size: 146 1280 Fragments arranged by size: 1583 1280 1064 511 374 371 146 140 138 100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2289 2502 2675 3770 4367 5154 5422 Size: 393 657 213 173 1095 597 787 268 Cuts at: 5422 1239 Size: 1524 Fragments arranged by size: 1524 1095 787 657 597 393 268 213 173 EcoNI CCTnn'n_nnAGG Cuts at: 2317 2654 2317 Size: 337 5370 EcoO109I rG'GnC_Cy Cuts at: 2236 2237 2302 2603 2808 2236 Size: 1 65 301 205 5135 Fragments arranged by size: 5135 301 205 65 1 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FspI TGC'GCA Cuts at: 2153 2708 4213 2153 Size: 555 1505 3647 Fragments arranged by size: 3647 1505 555 HaeII r_GCGC'y Cuts at: 1678 4854 1678 Size: 3176 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2765 2797 4348 4380 4422 Size: 32 135 32 1193 32 1551 32 42 Cuts at: 4422 4454 1373 Size: 32 2626 Fragments arranged by size: 2626 1551 1193 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2375 2757 4821 5005 1305 Size: 1070 382 2064 184 2007 Fragments arranged by size: 2064 2007 1070 382 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3885 Fragments arranged by size: 3885 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3665 4024 4183 518 Size: 648 2499 359 159 2042 Fragments arranged by size: 2499 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2545 2557 2572 3123 3800 4948 5193 Size: 412 527 12 15 551 677 1148 245 Cuts at: 5193 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 551 527 412 245 15 12 NaeI GCC'GGC Cuts at: 2867 2867 Size: 5707 NcoI C'CATG_G Cuts at: 513 1065 2004 2256 2853 513 Size: 552 939 252 597 3367 Fragments arranged by size: 3367 939 597 552 252 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NgoMIV G'CCGG_C Cuts at: 2865 2865 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 2070 2086 2802 3065 4610 1132 Size: 97 841 16 716 263 1545 2229 Fragments arranged by size: 2229 1545 841 716 263 97 16 PasI CC'CwG_GG Cuts at: 1348 2428 1348 Size: 1080 4627 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 2548 2686 1108 Size: 1440 138 4129 Fragments arranged by size: 4129 1440 138 PfoI T'CCnGG_A Cuts at: 2283 2283 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PmlI CAC'GTG Cuts at: 2917 2917 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2302 2603 2302 Size: 301 5406 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PspOMI G'GGCC_C Cuts at: 2236 2807 2236 Size: 571 5136 PstI C_TGCA'G Cuts at: 830 3059 830 Size: 2229 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2557 2572 2557 Size: 15 5692 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SanDI GG'GwC_CC Cuts at: 2603 2603 Size: 5707 SapI GCTCTTCn'nnn_ Cuts at: 2265 2373 2265 Size: 108 5599 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SexAI A'CCwGG_T Cuts at: 2245 2245 Size: 5707 SfcI C'TryA_G Cuts at: 826 1045 2764 3055 4190 4871 5062 826 Size: 219 1719 291 1135 681 191 1471 Fragments arranged by size: 1719 1471 1135 681 291 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2843 2843 Size: 5707 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 2806 3034 1872 Size: 934 228 4545 Fragments arranged by size: 4545 934 228 SmlI C'TyrA_G Cuts at: 820 1017 1943 3811 4712 4974 820 Size: 197 926 1868 901 262 1553 Fragments arranged by size: 1868 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 2070 2802 3065 1132 Size: 938 732 263 3774 Fragments arranged by size: 3774 938 732 263 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2004 2256 2853 3146 513 Size: 552 939 252 597 293 3074 Fragments arranged by size: 3074 939 597 552 293 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2116 3734 3751 3904 4089 4534 325 Size: 1255 536 1618 17 153 185 445 1498 Fragments arranged by size: 1618 1498 1255 536 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3953 5665 96 Size: 274 80 33 51 1443 1976 1712 138 Fragments arranged by size: 1976 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2892 2924 5667 5699 2892 Size: 32 2743 32 2900 Fragments arranged by size: 2900 2743 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2067 2067 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 2804 3032 1870 Size: 934 228 4545 Fragments arranged by size: 4545 934 228 XmnI GAAnn'nnTTC Cuts at: 3836 3836 Size: 5707 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SfoI SrfI SwaI