(Linear) (Six-base) MAPSORT of: KIEE1820.seq Check: 6001 from: 1 to: 1002 KIEE1820.orf With 182 enzymes: SgfI * July 15, 2010 18:48 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 284 1002 Size: 284 718 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 539 571 1002 Size: 539 32 431 Fragments arranged by size: 539 431 32 AlwNI CAG_nnn'CTG Cuts at: 0 262 543 681 1002 Size: 262 281 138 321 Fragments arranged by size: 321 281 262 138 ApaI G_GGCC'C Cuts at: 0 235 806 1002 Size: 235 571 196 Fragments arranged by size: 571 235 196 ApaLI G'TGCA_C Cuts at: 0 92 913 1002 Size: 92 821 89 Fragments arranged by size: 821 92 89 AvaI C'yCGr_G Cuts at: 0 799 1002 Size: 799 203 BamHI G'GATC_C Cuts at: 0 932 1002 Size: 932 70 BanI G'GyrC_C Cuts at: 0 244 883 1002 Size: 244 639 119 Fragments arranged by size: 639 244 119 BanII G_rGCy'C Cuts at: 0 235 278 806 827 1002 Size: 235 43 528 21 175 Fragments arranged by size: 528 235 175 43 21 BbsI GAAGACnn'nnnn_ Cuts at: 0 290 403 1002 Size: 290 113 599 Fragments arranged by size: 599 290 113 BbvCI CC'TCA_GC Cuts at: 0 292 1002 Size: 292 710 BglI GCCn_nnn'nGGC Cuts at: 0 15 253 838 1002 Size: 15 238 585 164 Fragments arranged by size: 585 238 164 15 BlpI GC'TnA_GC Cuts at: 0 856 1002 Size: 856 146 Bme1580I G_kGCm'C Cuts at: 0 96 235 806 886 917 1002 Size: 96 139 571 80 31 85 Fragments arranged by size: 571 139 96 85 80 31 BmrI ACTGGGnnnn_n' Cuts at: 0 136 1002 Size: 136 866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 497 670 1002 Size: 497 173 332 Fragments arranged by size: 497 332 173 Bpu10I CC'TnA_GC Cuts at: 0 292 391 1002 Size: 292 99 611 Fragments arranged by size: 611 292 99 BsaI GGTCTCn'nnnn_ Cuts at: 0 192 341 1002 Size: 192 149 661 Fragments arranged by size: 661 192 149 BsaAI yAC'GTr Cuts at: 0 912 1002 Size: 912 90 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 210 231 446 667 670 1002 Size: 210 21 215 221 3 332 Fragments arranged by size: 332 221 215 210 21 3 BseYI C'CCAG_C Cuts at: 0 13 65 97 119 270 504 536 580 Size: 13 52 32 22 151 234 32 44 Cuts at: 580 876 950 1002 Size: 296 74 52 Fragments arranged by size: 296 234 151 74 52 52 44 32 32 22 13 BsiHKAI G_wGCw'C Cuts at: 0 96 911 917 1002 Size: 96 815 6 85 Fragments arranged by size: 815 96 85 6 Bsp1286I G_dGCh'C Cuts at: 0 96 235 278 806 827 886 911 917 Size: 96 139 43 528 21 59 25 6 Cuts at: 917 1002 Size: 85 Fragments arranged by size: 528 139 96 85 59 43 25 21 6 BsrFI r'CCGG_y Cuts at: 0 124 860 1002 Size: 124 736 142 Fragments arranged by size: 736 142 124 BstBI TT'CG_AA Cuts at: 0 402 1002 Size: 402 600 BstYI r'GATC_y Cuts at: 0 932 1002 Size: 932 70 Bsu36I CC'TnA_GG Cuts at: 0 313 1002 Size: 313 689 BtgI C'CryG_G Cuts at: 0 251 848 942 977 1002 Size: 251 597 94 35 25 Fragments arranged by size: 597 251 94 35 25 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 193 228 1002 Size: 193 35 774 Fragments arranged by size: 774 193 35 EaeI y'GGCC_r Cuts at: 0 52 869 890 1002 Size: 52 817 21 112 Fragments arranged by size: 817 112 52 21 EarI CTCTTCn'nnn_ Cuts at: 0 260 368 1002 Size: 260 108 634 Fragments arranged by size: 634 260 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 95 466 566 706 1002 Size: 95 371 100 140 296 Fragments arranged by size: 371 296 140 100 95 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 284 497 670 1002 Size: 284 213 173 332 Fragments arranged by size: 332 284 213 173 EcoNI CCTnn'n_nnAGG Cuts at: 0 312 649 1002 Size: 312 337 353 Fragments arranged by size: 353 337 312 EcoO109I rG'GnC_Cy Cuts at: 0 231 232 297 598 803 1002 Size: 231 1 65 301 205 199 Fragments arranged by size: 301 231 205 199 65 1 FspI TGC'GCA Cuts at: 0 148 703 1002 Size: 148 555 299 Fragments arranged by size: 555 299 148 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 760 792 1002 Size: 760 32 210 Fragments arranged by size: 760 210 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 370 752 1002 Size: 370 382 250 Fragments arranged by size: 382 370 250 MspA1I CmG'CkG Cuts at: 0 13 540 552 567 1002 Size: 13 527 12 15 435 Fragments arranged by size: 527 435 15 13 12 NaeI GCC'GGC Cuts at: 0 862 1002 Size: 862 140 NcoI C'CATG_G Cuts at: 0 251 848 1002 Size: 251 597 154 Fragments arranged by size: 597 251 154 NgoMIV G'CCGG_C Cuts at: 0 860 1002 Size: 860 142 NspI r_CATG'y Cuts at: 0 65 81 797 1002 Size: 65 16 716 205 Fragments arranged by size: 716 205 65 16 PasI CC'CwG_GG Cuts at: 0 423 1002 Size: 423 579 PflMI CCAn_nnn'nTGG Cuts at: 0 543 681 1002 Size: 543 138 321 Fragments arranged by size: 543 321 138 PfoI T'CCnGG_A Cuts at: 0 278 1002 Size: 278 724 PmlI CAC'GTG Cuts at: 0 912 1002 Size: 912 90 PpuMI rG'GwC_Cy Cuts at: 0 297 598 1002 Size: 297 301 404 Fragments arranged by size: 404 301 297 PspOMI G'GGCC_C Cuts at: 0 231 802 1002 Size: 231 571 200 Fragments arranged by size: 571 231 200 PvuII CAG'CTG Cuts at: 0 552 567 1002 Size: 552 15 435 Fragments arranged by size: 552 435 15 SanDI GG'GwC_CC Cuts at: 0 598 1002 Size: 598 404 SapI GCTCTTCn'nnn_ Cuts at: 0 260 368 1002 Size: 260 108 634 Fragments arranged by size: 634 260 108 SexAI A'CCwGG_T Cuts at: 0 240 1002 Size: 240 762 SfcI C'TryA_G Cuts at: 0 759 1002 Size: 759 243 SfiI GGCCn_nnn'nGGCC Cuts at: 0 838 1002 Size: 838 164 SmaI CCC'GGG Cuts at: 0 801 1002 Size: 801 201 SphI G_CATG'C Cuts at: 0 65 797 1002 Size: 65 732 205 Fragments arranged by size: 732 205 65 StyI C'CwwG_G Cuts at: 0 251 848 1002 Size: 251 597 154 Fragments arranged by size: 597 251 154 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 111 1002 Size: 111 891 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 887 919 1002 Size: 887 32 83 Fragments arranged by size: 887 83 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 62 1002 Size: 62 940 XmaI C'CCGG_G Cuts at: 0 799 1002 Size: 799 203 Enzymes that do cut: AcuI AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BseRI BseYI BsiHKAI Bsp1286I BsrFI BstBI BstYI Bsu36I BtgI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI Hin4I MmeI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI PvuII SanDI SapI SexAI SfcI SfiI SmaI SphI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI BpuEI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I BtgZI BtsI ClaI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI