################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII AclI AcsI AflII AgeI AhdI AocI ApoI AscI AseI AsnI AspEI AspI AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco81I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MluNI MscI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 838 GGTACC G^GTACC AccI 37 GTCTAC GT^[AC][GT]AC AccIII 856 TCCGGA T^CCGGA AflIII 662 ACGTGT A^C[GA][CT]GT Alw44I 598 GTGCAC G^TGCAC AlwNI 480 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 656 AGCGCT AGC^GCT ApaI 433 GGGCCC GGGCC^C ApaLI 598 GTGCAC G^TGCAC Asp700I 120 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 838 GGTACC G^GTACC AspHI 598 GTGCAC G[AT]GC[AT]^C AviII 575 TGCGCA TGC^GCA BamHI 488 GGATCC G^GATCC BfaI 613 CTAG C^TAG BsaAI 661 TACGTG [CT]AC^GT[GA] BsaWI 856 TCCGGA [AT]^CCGG[AT] BseAI 856 TCCGGA T^CCGGA BseRI 289 GAGGAG GAGGAG(10/8) BsiEI 886 CGGCCG CG[GA][CT]^CG BsiHKAI 598 GTGCAC G[AT]GC[AT]^C BsmI 745 GAATGC GAATGC(1/-1) BspEI 856 TCCGGA T^CCGGA BspMI 550 ACCTGC ACCTGC(4/8) BssSI 49 CACGAG CACGAG(-5/-1) BstAPI 14 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 518 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 488 GGATCC [GA]^GATC[CT] DsaI 623 CCGTGG C^C[GA][CT]GG EaeI 886 CGGCCG [CT]^GGCC[GA] EagI 886 CGGCCG C^GGCCG EclXI 886 CGGCCG C^GGCCG Eco47III 656 AGCGCT AGC^GCT Eco52I 886 CGGCCG C^GGCCG EcoNI 342 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 575 TGCGCA TGC^GCA HaeII 656 AGCGCT [GA]GCGC^[CT] KpnI 838 GGTACC GGTAC^C MaeI 613 CTAG C^TAG MaeII 662 ACGT A^CGT MflI 488 GGATCC [GA]^GATC[CT] MroI 856 TCCGGA T^CCGGA MslI 816 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 368 GCCGGC GCC^GGC NgoMIV 368 GCCGGC G^CCGGC NspI 578 GCATGT [GA]CATG^[CT] PflMI 479 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 352 GAGTC GAGTC(4/5) StuI 257 AGGCCT AGG^CCT TfiI 762 GATTC G^A[AT]TC TspRI 438 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 479 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 488 GGATCC [GA]^GATC[CT] XmnI 120 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 501 GACGCC G[GA]^CG[CT]C 591 GACGCC AfaI 216 GTAC GT^AC 839 GTAC AvaI 75 CTCGGG C^[CT]CG[GA]G 402 CTCGGG BsaHI 501 GACGCC G[GA]^CG[CT]C 591 GACGCC BsgI 21 CTGCAC GTGCAG(16/14) 223 CTGCAC BsmAI 794 GAGAC GTCTC(1/5) 140 GTCTC BsmFI 85 GGGAC GGGAC(10/14) 537 GTCCC BsoBI 75 CTCGGG C^[CT]CG[GA]G 402 CTCGGG BsrI 790 ACTGG ACTGG(1/-1) 437 CCAGT BstEII 546 GGTGACC G^GT[ACGT]ACC 735 GGTCACC BstF5I 207 CATCC GGATG(2/0) 111 GGATG BstPI 546 GGTGACC G^GT[ACGT]ACC 735 GGTCACC DdeI 103 CTGAG C^T[ACGT]AG 688 CTGAG DpnII 163 GATC ^GATC 489 GATC EcoO65I 546 GGTGACC G^GT[ACGT]ACC 735 GGTCACC EcoT14I 392 CCAAGG C^C[AT][AT]GG 512 CCAAGG FokI 207 CATCC GGATG(9/13) 111 GGATG Hin1I 501 GACGCC G[GA]^CG[CT]C 591 GACGCC HinfI 352 GAGTC G^A[ACGT]TC 762 GATTC MboI 163 GATC ^GATC 489 GATC NdeII 163 GATC ^GATC 489 GATC PpuMI 85 GGGACCC [GA]G^G[AT]CC[CT] 444 GGGTCCT PstI 148 CTGCAG CTGCA^G 826 CTGCAG RsaI 216 GTAC GT^AC 839 GTAC Sau3AI 163 GATC ^GATC 489 GATC SfaNI 112 GATGC GCATC(5/9) 894 GATGC SfcI 148 CTGCAG C^T[GA][CT]AG 826 CTGCAG StyI 392 CCAAGG C^C[AT][AT]GG 512 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AluI AlwI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI TaqI TseI Tsp45I TthHB8I