(Circular) (Six-base) MAPSORT of: pFN21AB6388.seq Check: 9057 from: 1 to: 5608 pFN21AB6388 5608 bp With 182 enzymes: SgfI * October 18, 2013 12:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2043 2951 1937 Size: 106 908 4594 Fragments arranged by size: 4594 908 106 Acc65I G'GTAC_C Cuts at: 2843 2929 2843 Size: 86 5522 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 3671 5055 5323 1632 Size: 662 1377 1384 268 1917 Fragments arranged by size: 1917 1384 1377 662 268 AfeI AGC'GCT Cuts at: 2663 2663 Size: 5608 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 2667 4507 5484 1203 Size: 1464 1840 977 1327 Fragments arranged by size: 1840 1464 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AlwNI CAG_nnn'CTG Cuts at: 2490 3351 4923 2490 Size: 861 1572 3175 Fragments arranged by size: 3175 1572 861 ApaI G_GGCC'C Cuts at: 2442 2442 Size: 5608 ApaLI G'TGCA_C Cuts at: 2603 3665 4821 2603 Size: 1062 1156 3390 Fragments arranged by size: 3390 1156 1062 ApoI r'AATT_y Cuts at: 1493 2917 3143 3295 5404 1493 Size: 1424 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 226 152 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2080 2407 2933 1870 Size: 73 137 327 526 4545 Fragments arranged by size: 4545 526 327 137 73 BamHI G'GATC_C Cuts at: 2493 2938 3305 2493 Size: 445 367 4796 Fragments arranged by size: 4796 445 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 2411 2843 2929 4384 Size: 325 217 627 495 129 432 86 1455 Cuts at: 4384 618 Size: 1842 Fragments arranged by size: 1842 1455 627 495 432 325 217 129 86 BanII G_rGCy'C Cuts at: 1391 2442 2646 2861 2927 1391 Size: 1051 204 215 66 4072 Fragments arranged by size: 4072 1051 215 204 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3799 3833 1903 Size: 34 1862 34 3678 Fragments arranged by size: 3678 1862 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4219 136 Size: 107 122 71 631 605 2547 1525 Fragments arranged by size: 2547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5603 5603 Size: 5608 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 2285 2414 2442 2607 3669 4825 1790 Size: 495 129 28 165 1062 1156 2573 Fragments arranged by size: 2573 1156 1062 495 165 129 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2436 3339 4297 Size: 416 53 83 399 24 1019 903 958 Cuts at: 4297 442 Size: 1753 Fragments arranged by size: 1753 1019 958 903 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2822 2822 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 2369 2552 2817 4268 1239 Size: 998 132 183 265 1451 2579 Fragments arranged by size: 2579 1451 998 265 183 132 Bpu10I CC'TnA_GC Cuts at: 2693 2693 Size: 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 4598 4896 3733 Size: 865 298 4445 Fragments arranged by size: 4445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2668 3399 493 Size: 711 1464 731 2702 Fragments arranged by size: 2702 1464 731 711 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2507 2597 3415 3797 Size: 53 83 186 936 974 90 818 382 Cuts at: 3797 4432 275 Size: 635 1451 Fragments arranged by size: 1451 974 936 818 635 382 186 90 83 53 BsaWI w'CCGG_w Cuts at: 2861 4041 4439 4713 4860 5271 2861 Size: 1180 398 274 147 411 3198 Fragments arranged by size: 3198 1180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2342 2372 2525 2555 1178 Size: 30 165 30 939 30 153 30 4231 Fragments arranged by size: 4231 939 165 153 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2309 1186 Size: 1123 4485 BseYI C'CCAG_C Cuts at: 2483 4811 5307 2483 Size: 2328 496 2784 Fragments arranged by size: 2784 2328 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2011 2213 1163 Size: 573 275 202 4558 Fragments arranged by size: 4558 573 275 202 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2894 3058 3819 4847 1540 Size: 275 186 893 164 761 1028 2301 Fragments arranged by size: 2301 1028 893 761 275 186 164 BsiHKAI G_wGCw'C Cuts at: 2607 3669 3754 4825 2607 Size: 1062 85 1071 3390 Fragments arranged by size: 3390 1071 1062 85 BsmI GAATG_Cn' Cuts at: 2756 3119 3212 2756 Size: 363 93 5152 Fragments arranged by size: 5152 363 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2285 2414 2442 2607 2646 2861 2927 Size: 399 495 129 28 165 39 215 66 Cuts at: 2927 3669 3754 4825 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 495 399 215 165 129 85 66 39 28 BspEI T'CCGG_A Cuts at: 2861 5271 2861 Size: 2410 3198 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2564 2963 844 Size: 1720 399 3489 Fragments arranged by size: 3489 1720 399 BsrBI CCG'CTC Cuts at: 1394 1823 3495 1394 Size: 429 1672 3507 Fragments arranged by size: 3507 1672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4103 4277 65 Size: 1183 2855 174 1396 Fragments arranged by size: 2855 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2373 2846 2893 4252 4439 1700 Size: 110 563 473 47 1359 187 2869 Fragments arranged by size: 2869 1359 563 473 187 110 47 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 2054 3668 4680 2054 Size: 1614 1012 2982 Fragments arranged by size: 2982 1614 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2025 794 Size: 1231 4377 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 2551 2740 1184 Size: 1367 189 4052 Fragments arranged by size: 4052 1367 189 BstXI CCAn_nnnn'nTGG Cuts at: 2530 2530 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2493 2938 3305 3690 3707 5159 5245 5257 Size: 521 445 367 385 17 1452 86 12 Cuts at: 5257 5603 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 521 445 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2628 513 Size: 552 1563 3493 Fragments arranged by size: 3493 1563 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 3124 3918 3938 3124 Size: 794 20 4794 Fragments arranged by size: 4794 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2891 3055 3944 Size: 54 1425 50 275 18 1061 164 889 Cuts at: 3944 5573 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1061 889 275 164 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2891 3055 1537 Size: 275 1079 164 4090 Fragments arranged by size: 4090 1079 275 164 EarI CTCTTCn'nnn_ Cuts at: 1353 3538 1353 Size: 2185 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2294 2369 2552 2817 3671 4268 Size: 393 605 57 75 183 265 854 597 Cuts at: 4268 5055 5323 1239 Size: 787 268 1524 Fragments arranged by size: 1524 854 787 605 597 393 268 265 183 75 57 EcoNI CCTnn'n_nnAGG Cuts at: 2351 2351 Size: 5608 EcoO109I rG'GnC_Cy Cuts at: 2091 2401 2450 2091 Size: 310 49 5249 Fragments arranged by size: 5249 310 49 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FspI TGC'GCA Cuts at: 2582 4114 2582 Size: 1532 4076 HaeII r_GCGC'y Cuts at: 1678 2665 4755 1678 Size: 987 2090 2531 Fragments arranged by size: 2531 2090 987 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 2525 2557 4249 Size: 32 135 32 770 32 151 32 1692 Cuts at: 4249 4281 4323 4355 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1692 770 151 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2847 2933 2847 Size: 86 5522 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2455 2568 4722 4906 1305 Size: 1150 113 2154 184 2007 Fragments arranged by size: 2154 2007 1150 184 113 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3786 Fragments arranged by size: 3786 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2825 3566 3925 4084 518 Size: 648 1659 741 359 159 2042 Fragments arranged by size: 2042 1659 741 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2075 2278 2314 2836 3024 3701 4849 5094 Size: 469 203 36 522 188 677 1148 245 Cuts at: 5094 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 522 469 245 203 188 36 NaeI GCC'GGC Cuts at: 2375 2375 Size: 5608 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5056 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NgoMIV G'CCGG_C Cuts at: 2373 2373 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2587 2966 4511 1132 Size: 97 1358 379 1545 2229 Fragments arranged by size: 2229 1545 1358 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5608 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 2490 1108 Size: 1382 4226 PfoI T'CCnGG_A Cuts at: 2214 2346 2214 Size: 132 5476 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2091 2450 2091 Size: 359 5249 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PspOMI G'GGCC_C Cuts at: 2438 2438 Size: 5608 PstI C_TGCA'G Cuts at: 830 2157 2835 2960 830 Size: 1327 678 125 3478 Fragments arranged by size: 3478 1327 678 125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SanDI GG'GwC_CC Cuts at: 2091 2091 Size: 5608 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2153 2831 2956 4091 4772 4963 826 Size: 219 1108 678 125 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 681 678 219 191 125 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3712 4613 4875 820 Size: 197 926 1769 901 262 1553 Fragments arranged by size: 1769 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 2264 1517 Size: 747 4861 StyI C'CwwG_G Cuts at: 513 1065 2397 2517 3047 513 Size: 552 1332 120 530 3074 Fragments arranged by size: 3074 1332 552 530 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3635 3652 3805 3990 4435 325 Size: 1255 2055 17 153 185 445 1498 Fragments arranged by size: 2055 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2220 3854 5566 Size: 274 80 33 51 1443 243 1634 1712 Cuts at: 5566 96 Size: 138 Fragments arranged by size: 1712 1634 1443 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 2129 3737 2129 Size: 1608 4000 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI