################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SapI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 12 GGCGCC G[GA]^CG[CT]C AlwI 431 GGATC GGATC(4/5) AlwNI 682 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 239 AGCGCT AGC^GCT AvaI 63 CTCGGG C^[CT]CG[GA]G BbeI 12 GGCGCC GGCGC^C BbsI 356 GTCTTC GAAGAC(2/6) BlpI 57 GCTGAGC GC^T[ACGT]AGC Bpu1102I 57 GCTGAGC GC^T[ACGT]AGC BpuAI 356 GTCTTC GAAGAC(2/6) BsaHI 12 GGCGCC G[GA]^CG[CT]C BsaI 397 GAGACC GGTCTC(1/5) BsgI 613 CTGCAC GTGCAG(16/14) BsmAI 397 GAGAC GTCTC(1/5) BsoBI 63 CTCGGG C^[CT]CG[GA]G BsrBI 250 GAGCGG CCGCTC(-3/-3) BstAPI 282 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 245 GGATG GGATG(2/0) BstXI 496 CCACCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 57 GCTGAGC GC^T[ACGT]AGC DpnII 432 GATC ^GATC DrdI 118 GACTTCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 596 TGGCCG [CT]^GGCC[GA] Eco47III 239 AGCGCT AGC^GCT EcoNI 387 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 502 CCATGG C^C[AT][AT]GG FokI 245 GGATG GGATG(9/13) HgaI 627 GACGC GACGC(5/10) Hin1I 12 GGCGCC G[GA]^CG[CT]C KasI 12 GGCGCC G^GCGCC MboI 432 GATC ^GATC MboII 357 TCTTC GAAGA(8/7) MslI 497 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 12 GGCGCC GG^CGCC NcoI 502 CCATGG C^CATGG NdeII 432 GATC ^GATC NlaIII 503 CATG CATG^ Sau3AI 432 GATC ^GATC SfaNI 279 GATGC GCATC(5/9) SfoI 12 GGCGCC GGC^GCC SmlI 256 CTCAAG C^T[CT][GA]AG StyI 502 CCATGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 298 CGCG CG^CG 629 CGCG BcnI 452 CCCGG CC^[GC]GG 512 CCCGG BstUI 298 CGCG CG^CG 629 CGCG DdeI 58 CTGAG C^T[ACGT]AG 70 CTCAG HapII 453 CCGG C^CGG 513 CCGG HpaII 453 CCGG C^CGG 513 CCGG HphI 173 TCACC GGTGA(8/7) 323 TCACC MaeIII 172 GTCAC ^GT[ACGT]AC 322 GTCAC MspI 453 CCGG C^CGG 513 CCGG MvnI 298 CGCG CG^CG 629 CGCG NciI 452 CCCGG CC^[GC]GG 512 CCCGG PpuMI 76 GGGACCC [GA]G^G[AT]CC[CT] 683 AGGACCT PvuII 351 CAGCTG CAG^CTG 409 CAGCTG SacI 60 GAGCTC GAGCT^C 202 GAGCTC SbfI 189 CCTGCAGG CCTGCA^GG 390 CCTGCAGG Sse8387I 189 CCTGCAGG CCTGCA^GG 390 CCTGCAGG Tsp45I 172 GTCAC ^GT[GC]AC 322 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AspHI AvaII BanI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstNI Cac8I CfoI Cfr13I DraII DsaI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HhaI HinP1I HinfI ItaI MnlI MspA1I MvaI MwoI NlaIV PleI PstI Sau96I ScrFI SfcI TseI TspRI