(Circular) (Six-base) MAPSORT of: pFN21AE1725.seq Check: 4089 from: 1 to: 5410 pFN21AE1725 5410 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2653 2653 Size: 5410 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3220 278 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2753 1937 Size: 816 4594 Acc65I G'GTAC_C Cuts at: 2731 2731 Size: 5410 AclI AA'CG_TT Cuts at: 1563 3537 3910 1563 Size: 1974 373 3063 Fragments arranged by size: 3063 1974 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2447 3473 4857 5125 1632 Size: 815 1026 1384 268 1917 Fragments arranged by size: 1917 1384 1026 815 268 AfeI AGC'GCT Cuts at: 2246 2246 Size: 5410 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5213 AflIII A'CryG_T Cuts at: 1203 4309 5286 1203 Size: 3106 977 1327 Fragments arranged by size: 3106 1327 977 AgeI A'CCGG_T Cuts at: 4241 4241 Size: 5410 AhdI GACnn_n'nnGTC Cuts at: 4139 4139 Size: 5410 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5410 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5410 AlwNI CAG_nnn'CTG Cuts at: 2692 3153 4725 2692 Size: 461 1572 3377 Fragments arranged by size: 3377 1572 461 ApaLI G'TGCA_C Cuts at: 3467 4623 3467 Size: 1156 4254 ApoI r'AATT_y Cuts at: 1493 2719 2945 3097 5206 1493 Size: 1226 226 152 2109 1697 Fragments arranged by size: 2109 1697 1226 226 152 AseI AT'TA_AT Cuts at: 160 1032 3964 160 Size: 872 2932 1606 Fragments arranged by size: 2932 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2735 1870 Size: 73 125 667 4545 Fragments arranged by size: 4545 667 125 73 BamHI G'GATC_C Cuts at: 2740 3107 2740 Size: 367 5043 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2460 2613 2665 2731 Size: 325 217 627 230 443 153 52 66 Cuts at: 2731 4186 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 443 325 230 217 153 66 52 BanII G_rGCy'C Cuts at: 1391 2033 2069 2211 2729 1391 Size: 642 36 142 518 4072 Fragments arranged by size: 4072 642 518 142 36 BbeI G_GCGC'C Cuts at: 2021 2021 Size: 5410 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2354 928 Size: 971 455 3984 Fragments arranged by size: 3984 971 455 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3601 3635 1903 Size: 34 1664 34 3678 Fragments arranged by size: 3678 1664 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3302 4512 3302 Size: 1210 4200 BclI T'GATC_A Cuts at: 1548 1548 Size: 5410 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4021 136 Size: 107 122 71 631 605 2349 1525 Fragments arranged by size: 2349 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5405 5405 Size: 5410 BlpI GC'TnA_GC Cuts at: 2063 2827 2063 Size: 764 4646 Bme1580I G_kGCm'C Cuts at: 1790 2670 3471 4627 1790 Size: 880 801 1156 2573 Fragments arranged by size: 2573 1156 880 801 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5410 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2168 2673 3141 Size: 416 53 83 399 24 751 505 468 Cuts at: 3141 4099 442 Size: 958 1753 Fragments arranged by size: 1753 958 751 505 468 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2047 2318 2486 2546 4070 1239 Size: 808 271 168 60 1524 2579 Fragments arranged by size: 2579 1524 808 271 168 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2246 3535 4400 4698 2246 Size: 1289 865 298 2958 Fragments arranged by size: 2958 1289 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2396 4073 882 Size: 617 897 1677 2219 Fragments arranged by size: 2219 1677 897 617 BsaAI yAC'GTr Cuts at: 493 1204 3201 493 Size: 711 1997 2702 Fragments arranged by size: 2702 1997 711 BsaBI GATnn'nnATC Cuts at: 5404 5404 Size: 5410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 3217 3599 4234 Size: 53 83 186 936 485 1199 382 635 Cuts at: 4234 275 Size: 1451 Fragments arranged by size: 1451 1199 936 635 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3843 4241 4515 4662 5073 3843 Size: 398 274 147 411 4180 Fragments arranged by size: 4180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2459 2489 1178 Size: 30 165 30 1056 30 4099 Fragments arranged by size: 4099 1056 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2107 2219 2479 2555 1186 Size: 850 71 112 260 76 4041 Fragments arranged by size: 4041 850 260 112 76 71 BseYI C'CCAG_C Cuts at: 2273 2354 4613 5109 2273 Size: 81 2259 496 2574 Fragments arranged by size: 2574 2259 496 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2603 1163 Size: 573 867 3970 Fragments arranged by size: 3970 867 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2860 3621 4649 1540 Size: 275 186 859 761 1028 2301 Fragments arranged by size: 2301 1028 859 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2069 2211 2709 3471 3556 4627 2069 Size: 142 498 762 85 1071 2852 Fragments arranged by size: 2852 1071 762 498 142 85 BsmI GAATG_Cn' Cuts at: 2921 3014 2921 Size: 93 5317 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2069 2211 2670 2709 2729 3471 Size: 399 243 36 142 459 39 20 742 Cuts at: 3471 3556 4627 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 459 399 243 142 85 39 36 20 BspEI T'CCGG_A Cuts at: 5073 5073 Size: 5410 BspHI T'CATG_A Cuts at: 3299 5029 3299 Size: 1730 3680 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2166 2202 2653 2700 2765 844 Size: 1322 36 451 47 65 3489 Fragments arranged by size: 3489 1322 451 65 47 36 BsrBI CCG'CTC Cuts at: 1394 1823 2257 3297 1394 Size: 429 434 1040 3507 Fragments arranged by size: 3507 1040 434 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3905 4079 65 Size: 1183 2657 174 1396 Fragments arranged by size: 2657 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4054 4241 1700 Size: 110 2244 187 2869 Fragments arranged by size: 2869 2244 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5410 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5410 BssSI C'ACGA_G Cuts at: 3470 4482 3470 Size: 1012 4398 BstAPI GCAn_nnn'nTGC Cuts at: 794 2293 794 Size: 1499 3911 BstBI TT'CG_AA Cuts at: 4214 4214 Size: 5410 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5410 BstXI CCAn_nnnn'nTGG Cuts at: 2508 2508 Size: 5410 BstYI r'GATC_y Cuts at: 1972 2740 3107 3492 3509 4961 5047 5059 5405 Size: 768 367 385 17 1452 86 12 346 Cuts at: 5405 1972 Size: 1977 Fragments arranged by size: 1977 1452 768 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2181 2507 2583 513 Size: 552 1116 326 76 3340 Fragments arranged by size: 3340 1116 552 326 76 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4671 Fragments arranged by size: 4671 683 56 BtsI GCAGTG_nn' Cuts at: 2480 2926 3720 3740 2480 Size: 446 794 20 4150 Fragments arranged by size: 4150 794 446 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5410 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5375 DraI TTT'AAA Cuts at: 2714 2714 Size: 5410 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2129 4417 809 Size: 507 813 2288 1802 Fragments arranged by size: 2288 1802 813 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2601 2857 3746 Size: 54 1425 50 275 18 771 256 889 Cuts at: 3746 5375 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 771 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2857 1537 Size: 275 1045 4090 Fragments arranged by size: 4090 1045 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3340 1353 Size: 1987 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2653 3997 4371 4517 387 Size: 1064 138 1064 1344 374 146 1280 Fragments arranged by size: 1344 1280 1064 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2067 2209 2067 Size: 142 5268 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2318 2447 2486 2546 3473 4070 Size: 393 415 271 129 39 60 927 597 Cuts at: 4070 4857 5125 1239 Size: 787 268 1524 Fragments arranged by size: 1524 927 787 597 415 393 271 268 129 60 39 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2396 Size: 5410 EcoO109I rG'GnC_Cy Cuts at: 2082 2218 2689 2082 Size: 136 471 4803 Fragments arranged by size: 4803 471 136 EcoRI G'AATT_C Cuts at: 2719 3097 2719 Size: 378 5032 FspI TGC'GCA Cuts at: 3916 3916 Size: 5410 HaeII r_GCGC'y Cuts at: 1678 2021 2248 2426 4557 1678 Size: 343 227 178 2131 2531 Fragments arranged by size: 2531 2131 343 227 178 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4051 4083 4125 4157 1373 Size: 32 135 32 2479 32 42 32 2626 Fragments arranged by size: 2626 2479 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2754 3000 669 Size: 988 281 816 246 3079 Fragments arranged by size: 3079 988 816 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5410 HpaI GTT'AAC Cuts at: 3000 3000 Size: 5410 KasI G'GCGC_C Cuts at: 2017 2017 Size: 5410 KpnI G_GTAC'C Cuts at: 2735 2735 Size: 5410 MfeI C'AATT_G Cuts at: 3009 3009 Size: 5410 MluI A'CGCG_T Cuts at: 5286 5286 Size: 5410 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2125 2145 2214 4524 4708 1305 Size: 820 20 69 2310 184 2007 Fragments arranged by size: 2310 2007 820 184 69 20 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3588 Fragments arranged by size: 3588 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2506 3368 3727 3886 518 Size: 648 843 497 862 359 159 2042 Fragments arranged by size: 2042 862 843 648 497 359 159 MspA1I CmG'CkG Cuts at: 1606 2358 2416 2606 2826 3503 4651 4896 1606 Size: 752 58 190 220 677 1148 245 2120 Fragments arranged by size: 2120 1148 752 677 245 220 190 58 NarI GG'CG_CC Cuts at: 2018 2018 Size: 5410 NcoI C'CATG_G Cuts at: 513 1065 2507 513 Size: 552 1442 3416 Fragments arranged by size: 3416 1442 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5410 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5410 NotI GC'GGCC_GC Cuts at: 2857 2857 Size: 5410 NspI r_CATG'y Cuts at: 1132 1229 2768 4313 1132 Size: 97 1539 1545 2229 Fragments arranged by size: 2229 1545 1539 97 PasI CC'CwG_GG Cuts at: 1348 2654 1348 Size: 1306 4104 PciI A'CATG_T Cuts at: 4309 4309 Size: 5410 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5410 PmeI GTTT'AAAC Cuts at: 2714 2714 Size: 5410 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3479 3511 4992 5024 3479 Size: 32 1481 32 3865 Fragments arranged by size: 3865 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2082 2689 2082 Size: 607 4803 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5410 PsiI TTA'TAA Cuts at: 2980 2980 Size: 5410 PstI C_TGCA'G Cuts at: 830 2199 2400 2475 2762 830 Size: 1369 201 75 287 3478 Fragments arranged by size: 3478 1369 287 201 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5410 PvuII CAG'CTG Cuts at: 2358 2416 2358 Size: 58 5352 SacI G_AGCT'C Cuts at: 2069 2211 2069 Size: 142 5268 SalI G'TCGA_C Cuts at: 1936 2752 1936 Size: 816 4594 SanDI GG'GwC_CC Cuts at: 2082 2082 Size: 5410 SbfI CC_TGCA'GG Cuts at: 2199 2400 2762 2199 Size: 201 362 4847 Fragments arranged by size: 4847 362 201 ScaI AGT'ACT Cuts at: 3658 5370 3658 Size: 1712 3698 SfcI C'TryA_G Cuts at: 826 1045 2195 2396 2471 2758 3893 4574 4765 Size: 219 1150 201 75 287 1135 681 191 Cuts at: 4765 826 Size: 1471 Fragments arranged by size: 1471 1150 1135 681 287 219 201 191 75 SfoI GGC'GCC Cuts at: 2019 2019 Size: 5410 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5410 SmaI CCC'GGG Cuts at: 1872 2737 1872 Size: 865 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 3514 4415 4677 820 Size: 197 926 318 1253 901 262 1553 Fragments arranged by size: 1553 1253 926 901 318 262 197 SnaBI TAC'GTA Cuts at: 493 3201 493 Size: 2708 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5410 SphI G_CATG'C Cuts at: 1132 2768 1132 Size: 1636 3774 SspI AAT'ATT Cuts at: 5 52 3334 5 Size: 47 3282 2081 Fragments arranged by size: 3282 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5410 StyI C'CwwG_G Cuts at: 513 1065 2507 2849 513 Size: 552 1442 342 3074 Fragments arranged by size: 3074 1442 552 342 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3437 3454 3607 3792 4237 325 Size: 1255 1857 17 153 185 445 1498 Fragments arranged by size: 1857 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3656 5368 96 Size: 274 80 33 51 1443 1679 1712 138 Fragments arranged by size: 1712 1679 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2459 2491 5370 5402 2459 Size: 32 2879 32 2467 Fragments arranged by size: 2879 2467 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5410 XbaI T'CTAG_A Cuts at: 2746 2746 Size: 5410 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5410 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5410 XmaI C'CCGG_G Cuts at: 1870 2735 1870 Size: 865 4545 XmnI GAAnn'nnTTC Cuts at: 3539 3539 Size: 5410 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3218 276 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI