(Linear) (Six-base) MAPSORT of: KIEE1725.seq Check: 3603 from: 1 to: 705 KIEE1725.orf With 182 enzymes: SgfI * January 29, 2014 11:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 648 705 Size: 648 57 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 705 Size: 442 263 AfeI AGC'GCT Cuts at: 0 241 705 Size: 241 464 AlwNI CAG_nnn'CTG Cuts at: 0 687 705 Size: 687 18 AvaI C'yCGr_G Cuts at: 0 63 705 Size: 63 642 BanI G'GyrC_C Cuts at: 0 12 455 608 660 705 Size: 12 443 153 52 45 Fragments arranged by size: 443 153 52 45 12 BanII G_rGCy'C Cuts at: 0 28 64 206 705 Size: 28 36 142 499 Fragments arranged by size: 499 142 36 28 BbeI G_GCGC'C Cuts at: 0 16 705 Size: 16 689 BbsI GAAGACnn'nnnn_ Cuts at: 0 349 705 Size: 349 356 BlpI GC'TnA_GC Cuts at: 0 58 705 Size: 58 647 Bme1580I G_kGCm'C Cuts at: 0 665 705 Size: 665 40 BmrI ACTGGGnnnn_n' Cuts at: 0 163 668 705 Size: 163 505 37 Fragments arranged by size: 505 163 37 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 42 313 481 541 705 Size: 42 271 168 60 164 Fragments arranged by size: 271 168 164 60 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 705 Size: 241 464 BsaI GGTCTCn'nnnn_ Cuts at: 0 391 705 Size: 391 314 BsaHI Gr'CG_yC Cuts at: 0 13 705 Size: 13 692 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 454 484 705 Size: 454 30 221 Fragments arranged by size: 454 221 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 31 102 214 474 550 705 Size: 31 71 112 260 76 155 Fragments arranged by size: 260 155 112 76 71 31 BseYI C'CCAG_C Cuts at: 0 268 349 705 Size: 268 81 356 Fragments arranged by size: 356 268 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 598 705 Size: 598 107 BsiHKAI G_wGCw'C Cuts at: 0 64 206 704 705 Size: 64 142 498 1 Fragments arranged by size: 498 142 64 1 Bsp1286I G_dGCh'C Cuts at: 0 28 64 206 665 704 705 Size: 28 36 142 459 39 1 Fragments arranged by size: 459 142 39 36 28 1 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 161 197 648 695 705 Size: 161 36 451 47 10 Fragments arranged by size: 451 161 47 36 10 BsrBI CCG'CTC Cuts at: 0 252 705 Size: 252 453 BstAPI GCAn_nnn'nTGC Cuts at: 0 288 705 Size: 288 417 BstXI CCAn_nnnn'nTGG Cuts at: 0 503 705 Size: 503 202 BtgI C'CryG_G Cuts at: 0 176 502 578 705 Size: 176 326 76 127 Fragments arranged by size: 326 176 127 76 BtsI GCAGTG_nn' Cuts at: 0 475 705 Size: 475 230 DrdI GACnn_nn'nnGTC Cuts at: 0 124 705 Size: 124 581 EaeI y'GGCC_r Cuts at: 0 596 705 Size: 596 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 648 705 Size: 648 57 EcoICRI GAG'CTC Cuts at: 0 62 204 705 Size: 62 142 501 Fragments arranged by size: 501 142 62 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 42 313 442 481 541 705 Size: 42 271 129 39 60 164 Fragments arranged by size: 271 164 129 60 42 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 391 705 Size: 391 314 EcoO109I rG'GnC_Cy Cuts at: 0 77 213 684 705 Size: 77 136 471 21 Fragments arranged by size: 471 136 77 21 HaeII r_GCGC'y Cuts at: 0 16 243 421 705 Size: 16 227 178 284 Fragments arranged by size: 284 227 178 16 KasI G'GCGC_C Cuts at: 0 12 705 Size: 12 693 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 120 140 209 705 Size: 120 20 69 496 Fragments arranged by size: 496 120 69 20 MslI CAynn'nnrTG Cuts at: 0 501 705 Size: 501 204 MspA1I CmG'CkG Cuts at: 0 353 411 601 705 Size: 353 58 190 104 Fragments arranged by size: 353 190 104 58 NarI GG'CG_CC Cuts at: 0 13 705 Size: 13 692 NcoI C'CATG_G Cuts at: 0 502 705 Size: 502 203 PasI CC'CwG_GG Cuts at: 0 649 705 Size: 649 56 PpuMI rG'GwC_Cy Cuts at: 0 77 684 705 Size: 77 607 21 Fragments arranged by size: 607 77 21 PstI C_TGCA'G Cuts at: 0 194 395 470 705 Size: 194 201 75 235 Fragments arranged by size: 235 201 194 75 PvuII CAG'CTG Cuts at: 0 353 411 705 Size: 353 58 294 Fragments arranged by size: 353 294 58 SacI G_AGCT'C Cuts at: 0 64 206 705 Size: 64 142 499 Fragments arranged by size: 499 142 64 SanDI GG'GwC_CC Cuts at: 0 77 705 Size: 77 628 SbfI CC_TGCA'GG Cuts at: 0 194 395 705 Size: 194 201 310 Fragments arranged by size: 310 201 194 SfcI C'TryA_G Cuts at: 0 190 391 466 705 Size: 190 201 75 239 Fragments arranged by size: 239 201 190 75 SfoI GGC'GCC Cuts at: 0 14 705 Size: 14 691 SmlI C'TyrA_G Cuts at: 0 256 705 Size: 256 449 StyI C'CwwG_G Cuts at: 0 502 705 Size: 502 203 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 454 486 705 Size: 454 32 219 Fragments arranged by size: 454 219 32 Enzymes that do cut: AarI AcuI AfeI AlwNI AvaI BanI BanII BbeI BbsI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BstAPI BstXI BtgI BtsI DrdI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI MmeI MslI MspA1I NarI NcoI PasI PpuMI PstI PvuII SacI SanDI SbfI SfcI SfoI SmlI StyI TstI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EarI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI