################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AccI AccIII AclI AflII AgeI AhdI AocI AscI AseI AsnI Asp700I AspEI BalI BbeI BbrPI BclI BfrI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DrdI Eam1105I Eco81I EcoO65I EcoRV FbaI FseI HincII HindII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 1018 GACGTC GACGT^C Acc65I 1898 GGTACC G^GTACC AcsI 61 GAATTC [GA]^AATT[CT] AcyI 1018 GACGTC G[GA]^CG[CT]C AflIII 519 ACATGT A^C[GA][CT]GT AlwNI 1716 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 371 GGGCCC GGGCC^C ApoI 61 GAATTC [GA]^AATT[CT] Asp718I 1898 GGTACC G^GTACC AspI 448 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2163 CCCGAG C^[CT]CG[GA]G AviII 1154 TGCGCA TGC^GCA AvrII 1800 CCTAGG C^CTAGG BamHI 1983 GGATCC G^GATCC BciVI 402 GGATAC GTATCC(6/5) BfaI 1801 CTAG C^TAG BglII 710 AGATCT A^GATCT BlnI 1800 CCTAGG C^CTAGG BsaHI 1018 GACGTC G[GA]^CG[CT]C BsgI 2094 CTGCAC GTGCAG(16/14) BsoBI 2163 CCCGAG C^[CT]CG[GA]G BspLU11I 519 ACATGT A^CATGT BspMI 406 ACCTGC ACCTGC(4/8) BssHII 1822 GCGCGC G^CGCGC BstAPI 287 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 88 TTCGAA TT^CGAA DraI 6 TTTAAA TTT^AAA DraIII 500 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 987 CGGCCG C^GGCCG EclXI 987 CGGCCG C^GGCCG Eco52I 987 CGGCCG C^GGCCG EcoNI 2193 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 61 GAATTC G^AATTC EcoT22I 868 ATGCAT ATGCA^T FspI 1154 TGCGCA TGC^GCA Hin1I 1018 GACGTC G[GA]^CG[CT]C HindIII 1207 AAGCTT A^AGCTT KpnI 1898 GGTACC GGTAC^C MaeI 1801 CTAG C^TAG MseI 7 TTAA T^TAA NsiI 868 ATGCAT ATGCA^T NspI 519 ACATGT [GA]CATG^[CT] NspV 88 TTCGAA TT^CGAA PflFI 448 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 220 AGGACCC [GA]G^G[AT]CC[CT] PstI 139 CTGCAG CTGCA^G SacI 394 GAGCTC GAGCT^C SapI 84 GCTCTTC GCTCTTC(1/4) SexAI 475 ACCTGGT A^CC[AT]GGT SfuI 88 TTCGAA TT^CGAA SmlI 808 CTCAAG C^T[CT][GA]AG StuI 1844 AGGCCT AGG^CCT Tru9I 7 TTAA T^TAA Tth111I 448 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwI 1984 GATCC GGATC(4/5) 1983 GGATC Aor51HI 380 AGCGCT AGC^GCT 2018 AGCGCT BcgI 1878 CGAGGCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2086 CGAGCGTCCTGC BglI 787 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1382 GCCGAATCGGC BsiEI 432 CGACCG CG[GA][CT]^CG 987 CGGCCG BstYI 710 AGATCT [GA]^GATC[CT] 1983 GGATCC Eco47III 380 AGCGCT AGC^GCT 2018 AGCGCT FauI 1959 CCCGC CCCGC(4/6) 924 GCGGG HgaI 448 GACGC GACGC(5/10) 2089 GCGTC MflI 710 AGATCT [GA]^GATC[CT] 1983 GGATCC NaeI 985 GCCGGC GCC^GGC 1733 GCCGGC NgoMIV 985 GCCGGC G^CCGGC 1733 GCCGGC Tsp509I 62 AATT ^AATT 1120 AATT XcmI 473 CCACCTGGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2183 CCACCAGCTCCCTGG XhoII 710 AGATCT [GA]^GATC[CT] 1983 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AfaI AluI Alw44I ApaLI AspHI AvaII BanI BanII BbsI BbvCI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I