(Circular) (Six-base) MAPSORT of: pFN21AE2074.seq Check: 7280 from: 1 to: 6916 pFN21AE2074 6916 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3027 4726 278 Size: 53 83 186 936 1491 1699 2468 Fragments arranged by size: 2468 1699 1491 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4259 1937 Size: 2322 4594 Acc65I G'GTAC_C Cuts at: 3903 4237 3903 Size: 334 6582 AclI AA'CG_TT Cuts at: 1563 4215 5043 5416 1563 Size: 2652 828 373 3063 Fragments arranged by size: 3063 2652 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 4979 6363 6631 1632 Size: 653 2694 1384 268 1917 Fragments arranged by size: 2694 1917 1384 653 268 AfeI AGC'GCT Cuts at: 2387 4025 2387 Size: 1638 5278 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6719 AflIII A'CryG_T Cuts at: 1203 2524 5815 6792 1203 Size: 1321 3291 977 1327 Fragments arranged by size: 3291 1327 1321 977 AgeI A'CCGG_T Cuts at: 5747 5747 Size: 6916 AhdI GACnn_n'nnGTC Cuts at: 5645 5645 Size: 6916 AleI CACnn'nnGTG Cuts at: 1166 2774 3972 4078 1166 Size: 1608 1198 106 4004 Fragments arranged by size: 4004 1608 1198 106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2770 1184 Size: 1586 5330 AlwNI CAG_nnn'CTG Cuts at: 3726 4659 6231 3726 Size: 933 1572 4411 Fragments arranged by size: 4411 1572 933 ApaI G_GGCC'C Cuts at: 2380 2380 Size: 6916 ApaLI G'TGCA_C Cuts at: 3334 3559 4071 4973 6129 3334 Size: 225 512 902 1156 4121 Fragments arranged by size: 4121 1156 902 512 225 ApoI r'AATT_y Cuts at: 1493 2066 4225 4451 4603 6712 1493 Size: 573 2159 226 152 2109 1697 Fragments arranged by size: 2159 2109 1697 573 226 152 AseI AT'TA_AT Cuts at: 160 1032 5470 160 Size: 872 4438 1606 Fragments arranged by size: 4438 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 AvaI C'yCGr_G Cuts at: 1870 1943 4168 4241 1870 Size: 73 2225 73 4545 Fragments arranged by size: 4545 2225 73 73 AvrII C'CTAG_G Cuts at: 3805 3805 Size: 6916 BamHI G'GATC_C Cuts at: 3988 4246 4613 3988 Size: 258 367 6291 Fragments arranged by size: 6291 367 258 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 2651 3903 3928 4237 Size: 325 217 627 576 288 1252 25 309 Cuts at: 4237 5692 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1252 627 576 325 309 288 217 25 BanII G_rGCy'C Cuts at: 1391 2380 2403 3879 4179 4235 1391 Size: 989 23 1476 300 56 4072 Fragments arranged by size: 4072 1476 989 300 56 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2758 3019 3279 3508 4020 928 Size: 971 859 261 260 229 512 3824 Fragments arranged by size: 3824 971 859 512 261 260 229 BbvCI CC'TCA_GC Cuts at: 2368 3640 4181 2368 Size: 1272 541 5103 Fragments arranged by size: 5103 1272 541 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3872 3906 4080 4114 5107 Size: 34 50 34 1851 34 174 34 993 Cuts at: 5107 5141 1903 Size: 34 3678 Fragments arranged by size: 3678 1851 993 174 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2401 4808 6018 2401 Size: 2407 1210 3299 Fragments arranged by size: 3299 2407 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6916 BfrBI ATG'CAT Cuts at: 2875 2875 Size: 6916 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2798 3393 5527 Size: 107 122 71 631 605 1126 595 2134 Cuts at: 5527 136 Size: 1525 Fragments arranged by size: 2134 1525 1126 631 605 595 122 107 71 BglII A'GATC_T Cuts at: 2715 6911 2715 Size: 4196 2720 BlpI GC'TnA_GC Cuts at: 4333 4333 Size: 6916 Bme1580I G_kGCm'C Cuts at: 1790 2380 2584 3338 3563 4075 4977 6133 1790 Size: 590 204 754 225 512 902 1156 2573 Fragments arranged by size: 2573 1156 902 754 590 512 225 204 BmgBI CAC'GTC Cuts at: 1318 2359 2764 1318 Size: 1041 405 5470 Fragments arranged by size: 5470 1041 405 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2065 3573 4647 Size: 416 53 83 399 24 648 1508 1074 Cuts at: 4647 5605 442 Size: 958 1753 Fragments arranged by size: 1753 1508 1074 958 648 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6916 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2925 3466 3796 2925 Size: 541 330 6045 Fragments arranged by size: 6045 541 330 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2096 2264 2402 2696 3053 3943 4028 Size: 830 27 168 138 294 357 890 85 Cuts at: 4028 4135 5576 1239 Size: 107 1441 2579 Fragments arranged by size: 2579 1441 890 830 357 294 168 138 107 85 27 Bpu10I CC'TnA_GC Cuts at: 2368 3640 4181 2368 Size: 1272 541 5103 Fragments arranged by size: 5103 1272 541 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2798 5041 5906 6204 2798 Size: 2243 865 298 3510 Fragments arranged by size: 3510 2243 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5579 882 Size: 617 4080 2219 Fragments arranged by size: 4080 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4707 493 Size: 711 3503 2702 Fragments arranged by size: 3503 2702 711 BsaBI GATnn'nnATC Cuts at: 6910 6910 Size: 6916 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3024 4723 5105 5740 Size: 53 83 186 936 1491 1699 382 635 Cuts at: 5740 275 Size: 1451 Fragments arranged by size: 1699 1491 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5349 5747 6021 6168 6579 5349 Size: 398 274 147 411 5686 Fragments arranged by size: 5686 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6691 Fragments arranged by size: 6691 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2528 3349 3442 3500 3998 4055 4096 4220 Size: 1342 821 93 58 498 57 41 124 Cuts at: 4220 1186 Size: 3882 Fragments arranged by size: 3882 1342 821 498 124 93 58 57 41 BseYI C'CCAG_C Cuts at: 2629 6119 6615 2629 Size: 3490 496 2930 Fragments arranged by size: 3490 2930 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4084 1163 Size: 573 2348 3995 Fragments arranged by size: 3995 2348 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2440 2995 4366 5127 6155 1540 Size: 275 186 439 555 1371 761 1028 2301 Fragments arranged by size: 2301 1371 1028 761 555 439 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 2352 2403 3338 3563 3864 4075 4977 5062 Size: 261 51 935 225 301 211 902 85 Cuts at: 5062 6133 2091 Size: 1071 2874 Fragments arranged by size: 2874 1071 935 902 301 261 225 211 85 51 BsmI GAATG_Cn' Cuts at: 4427 4520 4427 Size: 93 6823 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2352 2380 2403 2584 3338 3563 Size: 399 301 261 28 23 181 754 225 Cuts at: 3563 3864 3879 4075 4179 4235 4977 5062 6133 Size: 301 15 196 104 56 742 85 1071 Cuts at: 6133 1391 Size: 2174 Fragments arranged by size: 2174 1071 754 742 399 301 301 261 225 196 181 104 85 56 28 23 15 BspEI T'CCGG_A Cuts at: 6579 6579 Size: 6916 BspHI T'CATG_A Cuts at: 4805 6535 4805 Size: 1730 5186 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2420 4271 844 Size: 1576 1851 3489 Fragments arranged by size: 3489 1851 1576 BsrBI CCG'CTC Cuts at: 1394 1823 4803 1394 Size: 429 2980 3507 Fragments arranged by size: 3507 2980 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5411 5585 65 Size: 1183 4163 174 1396 Fragments arranged by size: 4163 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2648 2990 3550 3666 3738 3810 4056 Size: 110 838 342 560 116 72 72 246 Cuts at: 4056 5560 5747 1700 Size: 1504 187 2869 Fragments arranged by size: 2869 1504 838 560 342 246 187 116 110 72 72 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6916 BssHII G'CGCG_C Cuts at: 1925 3827 1925 Size: 1902 5014 BssSI C'ACGA_G Cuts at: 4976 5988 4976 Size: 1012 5904 BstAPI GCAn_nnn'nTGC Cuts at: 794 2298 794 Size: 1504 5412 BstBI TT'CG_AA Cuts at: 2094 5720 2094 Size: 3626 3290 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6916 BstXI CCAn_nnnn'nTGG Cuts at: 2629 2942 4198 2629 Size: 313 1256 5347 Fragments arranged by size: 5347 1256 313 BstYI r'GATC_y Cuts at: 1972 2715 3988 4246 4613 4998 5015 6467 6553 Size: 743 1273 258 367 385 17 1452 86 Cuts at: 6553 6565 6911 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1273 743 385 367 346 258 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2429 2655 3496 3914 513 Size: 552 1364 226 841 418 3515 Fragments arranged by size: 3515 1364 841 552 418 226 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3613 487 Size: 683 56 2387 3790 Fragments arranged by size: 3790 2387 683 56 BtsI GCAGTG_nn' Cuts at: 2036 2091 2752 3415 4432 5226 5246 2036 Size: 55 661 663 1017 794 20 3706 Fragments arranged by size: 3706 1017 794 663 661 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6916 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2107 2142 529 Size: 35 1543 35 5303 Fragments arranged by size: 5303 1543 35 35 DraI TTT'AAA Cuts at: 2013 4220 2013 Size: 2207 4709 DraIII CAC_nnn'GTG Cuts at: 2510 2510 Size: 6916 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5923 809 Size: 507 4607 1802 Fragments arranged by size: 4607 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2992 3180 3917 Size: 54 1425 50 275 18 1162 188 737 Cuts at: 3917 4082 4143 4363 5252 6881 8 Size: 165 61 220 889 1629 43 Fragments arranged by size: 1629 1425 1162 889 737 275 220 188 165 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2992 4363 1537 Size: 275 1180 1371 4090 Fragments arranged by size: 4090 1371 1180 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 2261 3284 3404 4846 1353 Size: 743 165 1023 120 1442 3423 Fragments arranged by size: 3423 1442 1023 743 165 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3374 3902 5503 5877 6023 387 Size: 1064 138 1785 528 1601 374 146 1280 Fragments arranged by size: 1785 1601 1280 1064 528 374 146 138 EcoICRI GAG'CTC Cuts at: 2401 2401 Size: 6916 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2096 2264 2285 2402 2696 3053 Size: 393 437 27 168 21 117 294 357 Cuts at: 3053 3943 4028 4135 4979 5576 6363 6631 1239 Size: 890 85 107 844 597 787 268 1524 Fragments arranged by size: 1524 890 844 787 597 437 393 357 294 268 168 117 107 85 27 21 EcoNI CCTnn'n_nnAGG Cuts at: 4202 4202 Size: 6916 EcoO109I rG'GnC_Cy Cuts at: 2226 2248 2336 2377 2226 Size: 22 88 41 6765 Fragments arranged by size: 6765 88 41 22 EcoRI G'AATT_C Cuts at: 2066 4225 4603 2066 Size: 2159 378 4379 Fragments arranged by size: 4379 2159 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3292 3292 Size: 6916 FspI TGC'GCA Cuts at: 3161 5422 3161 Size: 2261 4655 HaeII r_GCGC'y Cuts at: 1678 2320 2389 2638 3778 3842 4027 6063 1678 Size: 642 69 249 1140 64 185 2036 2531 Fragments arranged by size: 2531 2036 1140 642 249 185 69 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3344 3376 3680 3712 5557 Size: 32 135 32 1772 32 304 32 1845 Cuts at: 5557 5589 5631 5663 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1845 1772 304 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4260 4506 669 Size: 988 281 2322 246 3079 Fragments arranged by size: 3079 2322 988 281 246 HindIII A'AGCT_T Cuts at: 748 3212 748 Size: 2464 4452 HpaI GTT'AAC Cuts at: 4506 4506 Size: 6916 KpnI G_GTAC'C Cuts at: 3907 4241 3907 Size: 334 6582 MfeI C'AATT_G Cuts at: 4515 4515 Size: 6916 MluI A'CGCG_T Cuts at: 6792 6792 Size: 6916 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2542 3033 3807 6030 6214 1305 Size: 1237 491 774 2223 184 2007 Fragments arranged by size: 2223 2007 1237 774 491 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5094 Fragments arranged by size: 5094 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 2541 2774 2940 3284 3566 3972 Size: 648 1257 118 233 166 344 282 406 Cuts at: 3972 4078 4874 5233 5392 518 Size: 106 796 359 159 2042 Fragments arranged by size: 2042 1257 796 648 406 359 344 282 233 166 159 118 106 MspA1I CmG'CkG Cuts at: 1606 3313 3760 4006 4116 4332 5009 6157 6402 Size: 1707 447 246 110 216 677 1148 245 Cuts at: 6402 1606 Size: 2120 Fragments arranged by size: 2120 1707 1148 677 447 246 245 216 110 NaeI GCC'GGC Cuts at: 2992 3740 2992 Size: 748 6168 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6364 NdeI CA'TA_TG Cuts at: 387 387 Size: 6916 NgoMIV G'CCGG_C Cuts at: 2990 3738 2990 Size: 748 6168 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6916 NotI GC'GGCC_GC Cuts at: 4363 4363 Size: 6916 NsiI A_TGCA'T Cuts at: 2877 2877 Size: 6916 NspI r_CATG'y Cuts at: 1132 1229 2528 4274 5819 1132 Size: 97 1299 1746 1545 2229 Fragments arranged by size: 2229 1746 1545 1299 97 PasI CC'CwG_GG Cuts at: 1348 3898 4198 1348 Size: 2550 300 4066 Fragments arranged by size: 4066 2550 300 PciI A'CATG_T Cuts at: 2524 5815 2524 Size: 3291 3625 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6916 PfoI T'CCnGG_A Cuts at: 2352 2916 3030 2352 Size: 564 114 6238 Fragments arranged by size: 6238 564 114 PmeI GTTT'AAAC Cuts at: 4220 4220 Size: 6916 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4985 5017 6498 6530 4985 Size: 32 1481 32 5371 Fragments arranged by size: 5371 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2226 2226 Size: 6916 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6916 PsiI TTA'TAA Cuts at: 4486 4486 Size: 6916 PspOMI G'GGCC_C Cuts at: 2376 2376 Size: 6916 PstI C_TGCA'G Cuts at: 830 2148 4268 830 Size: 1318 2120 3478 Fragments arranged by size: 3478 2120 1318 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6916 SacI G_AGCT'C Cuts at: 2403 2403 Size: 6916 SalI G'TCGA_C Cuts at: 1936 4258 1936 Size: 2322 4594 SapI GCTCTTCn'nnn_ Cuts at: 2096 2096 Size: 6916 SbfI CC_TGCA'GG Cuts at: 4268 4268 Size: 6916 ScaI AGT'ACT Cuts at: 5164 6876 5164 Size: 1712 5204 SexAI A'CCwGG_T Cuts at: 2480 2480 Size: 6916 SfcI C'TryA_G Cuts at: 826 1045 2144 2595 3718 4264 5399 6080 6271 Size: 219 1099 451 1123 546 1135 681 191 Cuts at: 6271 826 Size: 1471 Fragments arranged by size: 1471 1135 1123 1099 681 546 451 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6916 SmaI CCC'GGG Cuts at: 1872 4243 1872 Size: 2371 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2813 5020 5921 6183 820 Size: 197 926 870 2207 901 262 1553 Fragments arranged by size: 2207 1553 926 901 870 262 197 SnaBI TAC'GTA Cuts at: 493 4707 493 Size: 4214 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6916 SphI G_CATG'C Cuts at: 1132 4274 1132 Size: 3142 3774 SspI AAT'ATT Cuts at: 5 52 4840 5 Size: 47 4788 2081 Fragments arranged by size: 4788 2081 47 StuI AGG'CCT Cuts at: 1517 3851 1517 Size: 2334 4582 StyI C'CwwG_G Cuts at: 513 1065 3508 3536 3805 3846 4050 4355 513 Size: 552 2443 28 269 41 204 305 3074 Fragments arranged by size: 3074 2443 552 305 269 204 41 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2114 2351 2480 2630 3353 4943 4960 Size: 1255 534 237 129 150 723 1590 17 Cuts at: 4960 5113 5298 5743 325 Size: 153 185 445 1498 Fragments arranged by size: 1590 1498 1255 723 534 445 237 185 153 150 129 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5162 6874 96 Size: 274 80 33 51 1443 3185 1712 138 Fragments arranged by size: 3185 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6876 6908 6876 Size: 32 6884 Tth111I GACn'n_nGTC Cuts at: 1316 2456 1316 Size: 1140 5776 XbaI T'CTAG_A Cuts at: 4252 4252 Size: 6916 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2485 4195 2485 Size: 1710 5206 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6916 XmaI C'CCGG_G Cuts at: 1870 4241 1870 Size: 2371 4545 XmnI GAAnn'nnTTC Cuts at: 5045 5045 Size: 6916 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3025 4724 276 Size: 53 83 186 936 1491 1699 2468 Fragments arranged by size: 2468 1699 1491 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI