(Linear) (Six-base) MAPSORT of: KIEE2074.seq Check: 8699 from: 1 to: 2211 KIEE2074.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AatII G_ACGT'C Cuts at: 0 1022 2211 Size: 1022 1189 Acc65I G'GTAC_C Cuts at: 0 1898 2211 Size: 1898 313 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 2211 Size: 280 1931 AfeI AGC'GCT Cuts at: 0 382 2020 2211 Size: 382 1638 191 Fragments arranged by size: 1638 382 191 AflIII A'CryG_T Cuts at: 0 519 2211 Size: 519 1692 AleI CACnn'nnGTG Cuts at: 0 769 1967 2073 2211 Size: 769 1198 106 138 Fragments arranged by size: 1198 769 138 106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 765 2211 Size: 765 1446 AlwNI CAG_nnn'CTG Cuts at: 0 1721 2211 Size: 1721 490 ApaI G_GGCC'C Cuts at: 0 375 2211 Size: 375 1836 ApaLI G'TGCA_C Cuts at: 0 1329 1554 2066 2211 Size: 1329 225 512 145 Fragments arranged by size: 1329 512 225 145 ApoI r'AATT_y Cuts at: 0 61 2211 Size: 61 2150 AvaI C'yCGr_G Cuts at: 0 2163 2211 Size: 2163 48 AvrII C'CTAG_G Cuts at: 0 1800 2211 Size: 1800 411 BamHI G'GATC_C Cuts at: 0 1983 2211 Size: 1983 228 BanI G'GyrC_C Cuts at: 0 358 646 1898 1923 2211 Size: 358 288 1252 25 288 Fragments arranged by size: 1252 358 288 288 25 BanII G_rGCy'C Cuts at: 0 375 398 1874 2174 2211 Size: 375 23 1476 300 37 Fragments arranged by size: 1476 375 300 37 23 BbsI GAAGACnn'nnnn_ Cuts at: 0 753 1014 1274 1503 2015 2211 Size: 753 261 260 229 512 196 Fragments arranged by size: 753 512 261 260 229 196 BbvCI CC'TCA_GC Cuts at: 0 363 1635 2176 2211 Size: 363 1272 541 35 Fragments arranged by size: 1272 541 363 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1867 1901 2075 2109 2211 Size: 1867 34 174 34 102 Fragments arranged by size: 1867 174 102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 396 2211 Size: 396 1815 BfrBI ATG'CAT Cuts at: 0 870 2211 Size: 870 1341 BglI GCCn_nnn'nGGC Cuts at: 0 793 1388 2211 Size: 793 595 823 Fragments arranged by size: 823 793 595 BglII A'GATC_T Cuts at: 0 710 2211 Size: 710 1501 Bme1580I G_kGCm'C Cuts at: 0 375 579 1333 1558 2070 2211 Size: 375 204 754 225 512 141 Fragments arranged by size: 754 512 375 225 204 141 BmgBI CAC'GTC Cuts at: 0 354 759 2211 Size: 354 405 1452 Fragments arranged by size: 1452 405 354 BmrI ACTGGGnnnn_n' Cuts at: 0 60 1568 2211 Size: 60 1508 643 Fragments arranged by size: 1508 643 60 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 920 1461 1791 2211 Size: 920 541 330 420 Fragments arranged by size: 920 541 420 330 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 91 259 397 691 1048 1938 2023 Size: 64 27 168 138 294 357 890 85 Cuts at: 2023 2130 2211 Size: 107 81 Fragments arranged by size: 890 357 294 168 138 107 85 81 64 27 Bpu10I CC'TnA_GC Cuts at: 0 363 1635 2176 2211 Size: 363 1272 541 35 Fragments arranged by size: 1272 541 363 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 793 2211 Size: 793 1418 BsaHI Gr'CG_yC Cuts at: 0 1019 2211 Size: 1019 1192 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 523 1344 1437 1495 1993 2050 2091 2211 Size: 523 821 93 58 498 57 41 120 Fragments arranged by size: 821 523 498 120 93 58 57 41 BseYI C'CCAG_C Cuts at: 0 624 2211 Size: 624 1587 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2079 2211 Size: 2079 132 BsiEI CG_ry'CG Cuts at: 0 435 990 2211 Size: 435 555 1221 Fragments arranged by size: 1221 555 435 BsiHKAI G_wGCw'C Cuts at: 0 86 347 398 1333 1558 1859 2070 2211 Size: 86 261 51 935 225 301 211 141 Fragments arranged by size: 935 301 261 225 211 141 86 51 Bsp1286I G_dGCh'C Cuts at: 0 86 347 375 398 579 1333 1558 1859 Size: 86 261 28 23 181 754 225 301 Cuts at: 1859 1874 2070 2174 2211 Size: 15 196 104 37 Fragments arranged by size: 754 301 261 225 196 181 104 86 37 28 23 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 415 2211 Size: 415 1796 BsrFI r'CCGG_y Cuts at: 0 643 985 1545 1661 1733 1805 2051 2211 Size: 643 342 560 116 72 72 246 160 Fragments arranged by size: 643 560 342 246 160 116 72 72 BssHII G'CGCG_C Cuts at: 0 1822 2211 Size: 1822 389 BstAPI GCAn_nnn'nTGC Cuts at: 0 293 2211 Size: 293 1918 BstBI TT'CG_AA Cuts at: 0 89 2211 Size: 89 2122 BstXI CCAn_nnnn'nTGG Cuts at: 0 624 937 2193 2211 Size: 624 313 1256 18 Fragments arranged by size: 1256 624 313 18 BstYI r'GATC_y Cuts at: 0 710 1983 2211 Size: 710 1273 228 Fragments arranged by size: 1273 710 228 BtgI C'CryG_G Cuts at: 0 424 650 1491 1909 2211 Size: 424 226 841 418 302 Fragments arranged by size: 841 424 418 302 226 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1608 2211 Size: 1608 603 BtsI GCAGTG_nn' Cuts at: 0 31 86 747 1410 2211 Size: 31 55 661 663 801 Fragments arranged by size: 801 663 661 55 31 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 102 137 2211 Size: 102 35 2074 Fragments arranged by size: 2074 102 35 DraI TTT'AAA Cuts at: 0 8 2211 Size: 8 2203 DraIII CAC_nnn'GTG Cuts at: 0 505 2211 Size: 505 1706 EaeI y'GGCC_r Cuts at: 0 987 1175 1912 2077 2138 2211 Size: 987 188 737 165 61 73 Fragments arranged by size: 987 737 188 165 73 61 EagI C'GGCC_G Cuts at: 0 987 2211 Size: 987 1224 EarI CTCTTCn'nnn_ Cuts at: 0 91 256 1279 1399 2211 Size: 91 165 1023 120 812 Fragments arranged by size: 1023 812 165 120 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1369 1897 2211 Size: 1369 528 314 Fragments arranged by size: 1369 528 314 EcoICRI GAG'CTC Cuts at: 0 396 2211 Size: 396 1815 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 91 259 280 397 691 1048 1938 Size: 64 27 168 21 117 294 357 890 Cuts at: 1938 2023 2130 2211 Size: 85 107 81 Fragments arranged by size: 890 357 294 168 117 107 85 81 64 27 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 2197 2211 Size: 2197 14 EcoO109I rG'GnC_Cy Cuts at: 0 221 243 331 372 2211 Size: 221 22 88 41 1839 Fragments arranged by size: 1839 221 88 41 22 EcoRI G'AATT_C Cuts at: 0 61 2211 Size: 61 2150 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1287 2211 Size: 1287 924 FspI TGC'GCA Cuts at: 0 1156 2211 Size: 1156 1055 HaeII r_GCGC'y Cuts at: 0 315 384 633 1773 1837 2022 2211 Size: 315 69 249 1140 64 185 189 Fragments arranged by size: 1140 315 249 189 185 69 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1339 1371 1675 1707 2211 Size: 1339 32 304 32 504 Fragments arranged by size: 1339 504 304 32 32 HindIII A'AGCT_T Cuts at: 0 1207 2211 Size: 1207 1004 KpnI G_GTAC'C Cuts at: 0 1902 2211 Size: 1902 309 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 537 1028 1802 2211 Size: 537 491 774 409 Fragments arranged by size: 774 537 491 409 MslI CAynn'nnrTG Cuts at: 0 418 536 769 935 1279 1561 1967 2073 Size: 418 118 233 166 344 282 406 106 Cuts at: 2073 2211 Size: 138 Fragments arranged by size: 418 406 344 282 233 166 138 118 106 MspA1I CmG'CkG Cuts at: 0 1308 1755 2001 2111 2211 Size: 1308 447 246 110 100 Fragments arranged by size: 1308 447 246 110 100 NaeI GCC'GGC Cuts at: 0 987 1735 2211 Size: 987 748 476 Fragments arranged by size: 987 748 476 NgoMIV G'CCGG_C Cuts at: 0 985 1733 2211 Size: 985 748 478 Fragments arranged by size: 985 748 478 NsiI A_TGCA'T Cuts at: 0 872 2211 Size: 872 1339 NspI r_CATG'y Cuts at: 0 523 2211 Size: 523 1688 PasI CC'CwG_GG Cuts at: 0 1893 2193 2211 Size: 1893 300 18 Fragments arranged by size: 1893 300 18 PciI A'CATG_T Cuts at: 0 519 2211 Size: 519 1692 PfoI T'CCnGG_A Cuts at: 0 347 911 1025 2211 Size: 347 564 114 1186 Fragments arranged by size: 1186 564 347 114 PpuMI rG'GwC_Cy Cuts at: 0 221 2211 Size: 221 1990 PspOMI G'GGCC_C Cuts at: 0 371 2211 Size: 371 1840 PstI C_TGCA'G Cuts at: 0 143 2211 Size: 143 2068 SacI G_AGCT'C Cuts at: 0 398 2211 Size: 398 1813 SapI GCTCTTCn'nnn_ Cuts at: 0 91 2211 Size: 91 2120 SexAI A'CCwGG_T Cuts at: 0 475 2211 Size: 475 1736 SfcI C'TryA_G Cuts at: 0 139 590 1713 2211 Size: 139 451 1123 498 Fragments arranged by size: 1123 498 451 139 SmlI C'TyrA_G Cuts at: 0 808 2211 Size: 808 1403 StuI AGG'CCT Cuts at: 0 1846 2211 Size: 1846 365 StyI C'CwwG_G Cuts at: 0 1503 1531 1800 1841 2045 2211 Size: 1503 28 269 41 204 166 Fragments arranged by size: 1503 269 204 166 41 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 109 346 475 625 1348 2211 Size: 109 237 129 150 723 863 Fragments arranged by size: 863 723 237 150 129 109 Tth111I GACn'n_nGTC Cuts at: 0 451 2211 Size: 451 1760 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 480 2190 2211 Size: 480 1710 21 Fragments arranged by size: 1710 480 21 ZraI GAC'GTC Cuts at: 0 1020 2211 Size: 1020 1191 Enzymes that do cut: AatII Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HindIII KpnI MmeI MslI MspA1I NaeI NgoMIV NsiI NspI PasI PciI PfoI PpuMI PspOMI PstI SacI SapI SexAI SfcI SmlI StuI StyI TaqII Tth111I XcmI ZraI Enzymes that do not cut: AarI AccI AclI AflII AgeI AhdI AloI AscI AseI AsiSI BaeI BbeI BclI BlpI BmtI BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstEII BstZ17I Bsu36I ClaI DrdI EcoRV FseI FspAI HincII HpaI KasI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XhoI XmaI XmnI