################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MfeI MluI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp45I Tsp509I Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 805 CGCG CG^CG AccIII 818 TCCGGA T^CCGGA AcyI 106 GGCGTC G[GA]^CG[CT]C AocI 525 CCTGAGG CC^T[ACGT]AGG ApaI 7 GGGCCC GGGCC^C Asp700I 846 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 366 GAGCTC G[AT]GC[AT]^C BbsI 931 GAAGAC GAAGAC(2/6) BglI 742 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 386 AGATCT A^GATCT BpuAI 931 GAAGAC GAAGAC(2/6) BsaBI 228 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 106 GGCGTC G[GA]^CG[CT]C BseAI 818 TCCGGA T^CCGGA BsgI 550 GTGCAG GTGCAG(16/14) BsiEI 879 CGATCG CG[GA][CT]^CG BsiHKAI 366 GAGCTC G[AT]GC[AT]^C BspEI 818 TCCGGA T^CCGGA BspHI 362 TCATGA T^CATGA BstUI 805 CGCG CG^CG BstXI 785 CCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 525 CCTGAGG CC^T[ACGT]AGG DrdI 451 GACGTTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 659 CCGTGG C^C[GA][CT]GG EaeI 1059 CGGCCA [CT]^GGCC[GA] Eco81I 525 CCTGAGG CC^T[ACGT]AGG HaeII 739 AGCGCC [GA]GCGC^[CT] HgaI 107 GCGTC GACGC(5/10) Hin1I 106 GGCGTC G[GA]^CG[CT]C MaeII 452 ACGT A^CGT MamI 228 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 818 TCCGGA T^CCGGA MslI 302 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 691 CCGCTG C[AC]G^C[GT]G MvnI 805 CGCG CG^CG PaeR7I 136 CTCGAG C^TCGAG PpuMI 500 AGGACCC [GA]G^G[AT]CC[CT] PshAI 796 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 637 CTGCAG CTGCA^G PvuI 879 CGATCG CGAT^CG RcaI 362 TCATGA T^CATGA SacI 366 GAGCTC GAGCT^C SfcI 637 CTGCAG C^T[GA][CT]AG TspRI 707 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 1004 CCAACACCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 136 CTCGAG C^TCGAG XmnI 846 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwNI 520 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 956 CAGATCCTG BamHI 261 GGATCC G^GATCC 272 GGATCC BanI 300 GGCACC G^G[CT][GA]CC 325 GGTGCC BpmI 1038 CTCCAG CTGGAG(16/14) 1015 CTGGAG BsaWI 95 TCCGGT [AT]^CCGG[AT] 818 TCCGGA BseRI 631 GAGGAG GAGGAG(10/8) 940 GAGGAG BsrBI 276 CCGCTC CCGCTC(-3/-3) 295 GAGCGG CfoI 666 GCGC GCG^C 740 GCGC DraII 500 AGGACCC [GA]G^G[ACGT]CC[CT] 808 GGGGCCT EcoO109I 500 AGGACCC [GA]G^G[ACGT]CC[CT] 808 GGGGCCT HhaI 666 GCGC GCG^C 740 GCGC HinP1I 666 GCGC G^CGC 740 GCGC SmlI 136 CTCGAG C^T[CT][GA]AG 997 CTTGAG StuI 398 AGGCCT AGG^CCT 1064 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI AvaII BanII BbvI BcnI BmyI BsaI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmaI StyI TaqI Tru9I TseI TthHB8I XhoII XmaCI XmaI