(Circular) (Six-base) MAPSORT of: pFN21AE4350.seq Check: 9411 from: 1 to: 5800 pFN21AE4350 5800 bp With 182 enzymes: SgfI * March 28, 2012 13:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2276 2320 2395 2725 3863 5247 5515 1632 Size: 644 44 75 330 1138 1384 268 1917 Fragments arranged by size: 1917 1384 1138 644 330 268 75 44 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 2311 1166 Size: 1145 4655 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2921 1184 Size: 1737 4063 AlwNI CAG_nnn'CTG Cuts at: 2530 2966 3543 5115 2530 Size: 436 577 1572 3215 Fragments arranged by size: 3215 1572 577 436 ApaI G_GGCC'C Cuts at: 2016 2016 Size: 5800 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 3109 3335 3487 5596 1493 Size: 1616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2141 2451 2509 3125 1870 Size: 73 89 109 310 58 616 4545 Fragments arranged by size: 4545 616 310 109 89 73 58 BamHI G'GATC_C Cuts at: 2266 2277 3130 3497 2266 Size: 11 853 367 4569 Fragments arranged by size: 4569 853 367 11 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2305 2330 3121 4576 618 Size: 325 217 627 518 25 791 1455 1842 Fragments arranged by size: 1842 1455 791 627 518 325 217 25 BanII G_rGCy'C Cuts at: 1391 2016 2025 2375 2663 2720 2973 2991 3009 Size: 625 9 350 288 57 253 18 18 Cuts at: 3009 3033 3119 1391 Size: 24 86 4072 Fragments arranged by size: 4072 625 350 288 253 86 57 24 18 18 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2943 928 Size: 971 1044 3785 Fragments arranged by size: 3785 1044 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2753 4411 136 Size: 107 122 71 631 605 1081 1658 1525 Fragments arranged by size: 1658 1525 1081 631 605 122 107 71 BglII A'GATC_T Cuts at: 2391 5795 2391 Size: 3404 2396 BlpI GC'TnA_GC Cuts at: 3217 3217 Size: 5800 Bme1580I G_kGCm'C Cuts at: 1790 2016 2292 2308 2516 3861 5017 1790 Size: 226 276 16 208 1345 1156 2573 Fragments arranged by size: 2573 1345 1156 276 226 208 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2723 3531 4489 Size: 416 53 83 399 24 1306 808 958 Cuts at: 4489 442 Size: 1753 Fragments arranged by size: 1753 1306 958 808 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3028 3041 4460 1239 Size: 1789 13 1419 2579 Fragments arranged by size: 2579 1789 1419 13 Bpu10I CC'TnA_GC Cuts at: 2967 2979 2985 2967 Size: 12 6 5782 Fragments arranged by size: 5782 12 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3023 3925 4790 5088 3023 Size: 902 865 298 3735 Fragments arranged by size: 3735 902 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2312 2470 3071 4463 882 Size: 617 813 158 601 1392 2219 Fragments arranged by size: 2219 1392 813 617 601 158 BsaAI yAC'GTr Cuts at: 493 1204 3591 493 Size: 711 2387 2702 Fragments arranged by size: 2702 2387 711 BsaBI GATnn'nnATC Cuts at: 2237 5794 2237 Size: 3557 2243 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 3607 3989 4624 Size: 53 83 186 936 579 1495 382 635 Cuts at: 4624 275 Size: 1451 Fragments arranged by size: 1495 1451 936 635 579 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2100 2823 4233 4631 4905 5052 5463 2100 Size: 723 1410 398 274 147 411 2437 Fragments arranged by size: 2437 1410 723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5575 Fragments arranged by size: 5575 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2651 2960 1186 Size: 1465 309 4026 Fragments arranged by size: 4026 1465 309 BseYI C'CCAG_C Cuts at: 2698 2789 2972 2996 3050 3056 5003 5499 2698 Size: 91 183 24 54 6 1947 496 2999 Fragments arranged by size: 2999 1947 496 183 91 54 24 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2576 1163 Size: 573 840 4387 Fragments arranged by size: 4387 840 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2887 3250 4011 5039 1540 Size: 275 186 886 363 761 1028 2301 Fragments arranged by size: 2301 1028 886 761 363 275 186 BsiHKAI G_wGCw'C Cuts at: 2375 3861 3946 5017 2375 Size: 1486 85 1071 3158 Fragments arranged by size: 3158 1486 1071 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2025 2292 2308 2375 2516 2663 Size: 399 226 9 267 16 67 141 147 Cuts at: 2663 2720 2973 2991 3009 3033 3119 3861 3946 Size: 57 253 18 18 24 86 742 85 Cuts at: 3946 5017 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 267 253 226 147 141 86 85 67 57 24 18 18 16 9 BspEI T'CCGG_A Cuts at: 2823 5463 2823 Size: 2640 3160 BspHI T'CATG_A Cuts at: 2367 3689 5419 2367 Size: 1322 1730 2748 Fragments arranged by size: 2748 1730 1322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3155 844 Size: 2311 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2283 2302 3687 1394 Size: 429 460 19 1385 3507 Fragments arranged by size: 3507 1385 460 429 19 BsrDI GCAATG_nn' Cuts at: 65 1248 4295 4469 65 Size: 1183 3047 174 1396 Fragments arranged by size: 3047 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4444 4631 1700 Size: 110 2634 187 2869 Fragments arranged by size: 2869 2634 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 3860 4872 3860 Size: 1012 4788 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5800 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 2797 2797 Size: 5800 BstYI r'GATC_y Cuts at: 1972 2028 2266 2277 2391 2962 3130 3497 3882 Size: 56 238 11 114 571 168 367 385 Cuts at: 3882 3899 5351 5437 5449 5795 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 571 385 367 346 238 168 114 86 56 17 12 11 Bsu36I CC'TnA_GG Cuts at: 2531 2531 Size: 5800 BtgI C'CryG_G Cuts at: 513 1065 2004 2664 513 Size: 552 939 660 3649 Fragments arranged by size: 3649 939 660 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 3316 4110 4130 3316 Size: 794 20 4986 Fragments arranged by size: 4986 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2462 4807 809 Size: 507 1146 2345 1802 Fragments arranged by size: 2345 1802 1146 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3064 3247 4136 Size: 54 1425 50 275 18 1234 183 889 Cuts at: 4136 5765 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1234 889 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3247 1537 Size: 275 1435 4090 Fragments arranged by size: 4090 1435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3730 1353 Size: 2377 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 4387 4761 4907 387 Size: 1064 138 585 2213 374 146 1280 Fragments arranged by size: 2213 1280 1064 585 374 146 138 EcoICRI GAG'CTC Cuts at: 2373 2373 Size: 5800 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2320 2395 2725 3028 3041 3863 Size: 393 644 44 75 330 303 13 822 Cuts at: 3863 4460 5247 5515 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 822 787 644 597 393 330 303 268 75 44 13 EcoO109I rG'GnC_Cy Cuts at: 2506 2814 2506 Size: 308 5492 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2425 2872 2425 Size: 447 5353 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 2748 4947 1678 Size: 1070 2199 2531 Fragments arranged by size: 2531 2199 1070 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2362 2394 4441 Size: 32 135 32 476 32 282 32 2047 Cuts at: 4441 4473 4515 4547 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2047 476 282 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3144 3390 669 Size: 988 281 1206 246 3079 Fragments arranged by size: 3079 1206 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2793 4914 5098 1305 Size: 1488 2121 184 2007 Fragments arranged by size: 2121 2007 1488 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3978 Fragments arranged by size: 3978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2311 3758 4117 4276 518 Size: 648 1145 1447 359 159 2042 Fragments arranged by size: 2042 1447 1145 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2698 3216 3893 5041 5286 1606 Size: 1092 518 677 1148 245 2120 Fragments arranged by size: 2120 1148 1092 677 518 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 3158 4703 1132 Size: 97 1929 1545 2229 Fragments arranged by size: 2229 1929 1545 97 PasI CC'CwG_GG Cuts at: 1348 2928 1348 Size: 1580 4220 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5800 PfoI T'CCnGG_A Cuts at: 2273 2343 2273 Size: 70 5730 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2506 2506 Size: 5800 PshAI GACnn'nnGTC Cuts at: 1613 2805 1613 Size: 1192 4608 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PspOMI G'GGCC_C Cuts at: 2012 2012 Size: 5800 PstI C_TGCA'G Cuts at: 830 2646 3152 830 Size: 1816 506 3478 Fragments arranged by size: 3478 1816 506 PvuI CG_AT'CG Cuts at: 2001 2887 2001 Size: 886 4914 SacI G_AGCT'C Cuts at: 2375 2375 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SfcI C'TryA_G Cuts at: 826 1045 2642 3148 4283 4964 5155 826 Size: 219 1597 506 1135 681 191 1471 Fragments arranged by size: 1597 1471 1135 681 506 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 2034 2453 2511 3127 1872 Size: 162 419 58 616 4545 Fragments arranged by size: 4545 616 419 162 58 SmlI C'TyrA_G Cuts at: 820 1017 1943 2141 3002 3904 4805 5067 820 Size: 197 926 198 861 902 901 262 1553 Fragments arranged by size: 1553 926 902 901 861 262 198 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2405 3071 1517 Size: 888 666 4246 Fragments arranged by size: 4246 888 666 StyI C'CwwG_G Cuts at: 513 1065 2004 2400 2418 2424 3239 513 Size: 552 939 396 18 6 815 3074 Fragments arranged by size: 3074 939 815 552 396 18 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2183 2318 3102 3827 3844 3997 4182 Size: 1255 603 135 784 725 17 153 185 Cuts at: 4182 4627 325 Size: 445 1498 Fragments arranged by size: 1498 1255 784 725 603 445 185 153 135 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 2352 4046 Size: 274 80 33 51 1443 156 219 1694 Cuts at: 4046 5758 96 Size: 1712 138 Fragments arranged by size: 1712 1694 1443 274 219 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5760 5792 5760 Size: 32 5768 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3016 3016 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 2141 1943 Size: 198 5602 XmaI C'CCGG_G Cuts at: 1870 2032 2451 2509 3125 1870 Size: 162 419 58 616 4545 Fragments arranged by size: 4545 616 419 162 58 XmnI GAAnn'nnTTC Cuts at: 2855 3929 2855 Size: 1074 4726 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI