(Linear) (Six-base) MAPSORT of: KIEE4350.seq Check: 7796 from: 1 to: 1095 KIEE4350.orf With 182 enzymes: SgfI * March 22, 2012 17:48 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 315 390 720 1095 Size: 271 44 75 330 375 Fragments arranged by size: 375 330 271 75 44 AleI CACnn'nnGTG Cuts at: 0 306 1095 Size: 306 789 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 916 1095 Size: 916 179 AlwNI CAG_nnn'CTG Cuts at: 0 525 961 1095 Size: 525 436 134 Fragments arranged by size: 525 436 134 ApaI G_GGCC'C Cuts at: 0 11 1095 Size: 11 1084 AvaI C'yCGr_G Cuts at: 0 27 136 446 504 1095 Size: 27 109 310 58 591 Fragments arranged by size: 591 310 109 58 27 BamHI G'GATC_C Cuts at: 0 261 272 1095 Size: 261 11 823 Fragments arranged by size: 823 261 11 BanI G'GyrC_C Cuts at: 0 300 325 1095 Size: 300 25 770 Fragments arranged by size: 770 300 25 BanII G_rGCy'C Cuts at: 0 11 20 370 658 715 968 986 1004 Size: 11 9 350 288 57 253 18 18 Cuts at: 1004 1028 1095 Size: 24 67 Fragments arranged by size: 350 288 253 67 57 24 18 18 11 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 938 1095 Size: 938 157 BglI GCCn_nnn'nGGC Cuts at: 0 748 1095 Size: 748 347 BglII A'GATC_T Cuts at: 0 386 1095 Size: 386 709 Bme1580I G_kGCm'C Cuts at: 0 11 287 303 511 1095 Size: 11 276 16 208 584 Fragments arranged by size: 584 276 208 16 11 BmrI ACTGGGnnnn_n' Cuts at: 0 718 1095 Size: 718 377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1023 1036 1095 Size: 1023 13 59 Fragments arranged by size: 1023 59 13 Bpu10I CC'TnA_GC Cuts at: 0 962 974 980 1095 Size: 962 12 6 115 Fragments arranged by size: 962 115 12 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1018 1095 Size: 1018 77 BsaI GGTCTCn'nnnn_ Cuts at: 0 307 465 1066 1095 Size: 307 158 601 29 Fragments arranged by size: 601 307 158 29 BsaBI GATnn'nnATC Cuts at: 0 232 1095 Size: 232 863 BsaHI Gr'CG_yC Cuts at: 0 107 1095 Size: 107 988 BsaWI w'CCGG_w Cuts at: 0 95 818 1095 Size: 95 723 277 Fragments arranged by size: 723 277 95 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 646 955 1095 Size: 646 309 140 Fragments arranged by size: 646 309 140 BseYI C'CCAG_C Cuts at: 0 693 784 967 991 1045 1051 1095 Size: 693 91 183 24 54 6 44 Fragments arranged by size: 693 183 91 54 44 24 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 1095 Size: 571 524 BsiEI CG_ry'CG Cuts at: 0 882 1095 Size: 882 213 BsiHKAI G_wGCw'C Cuts at: 0 370 1095 Size: 370 725 Bsp1286I G_dGCh'C Cuts at: 0 11 20 287 303 370 511 658 715 Size: 11 9 267 16 67 141 147 57 Cuts at: 715 968 986 1004 1028 1095 Size: 253 18 18 24 67 Fragments arranged by size: 267 253 147 141 67 67 57 24 18 18 16 11 9 BspEI T'CCGG_A Cuts at: 0 818 1095 Size: 818 277 BspHI T'CATG_A Cuts at: 0 362 1095 Size: 362 733 BsrBI CCG'CTC Cuts at: 0 278 297 1095 Size: 278 19 798 Fragments arranged by size: 798 278 19 BstXI CCAn_nnnn'nTGG Cuts at: 0 792 1095 Size: 792 303 BstYI r'GATC_y Cuts at: 0 23 261 272 386 957 1095 Size: 23 238 11 114 571 138 Fragments arranged by size: 571 238 138 114 23 11 Bsu36I CC'TnA_GG Cuts at: 0 526 1095 Size: 526 569 BtgI C'CryG_G Cuts at: 0 659 1095 Size: 659 436 DrdI GACnn_nn'nnGTC Cuts at: 0 457 1095 Size: 457 638 EaeI y'GGCC_r Cuts at: 0 1059 1095 Size: 1059 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 169 1095 Size: 169 926 EcoICRI GAG'CTC Cuts at: 0 368 1095 Size: 368 727 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 315 390 720 1023 1036 1095 Size: 271 44 75 330 303 13 59 Fragments arranged by size: 330 303 271 75 59 44 13 EcoO109I rG'GnC_Cy Cuts at: 0 501 809 1095 Size: 501 308 286 Fragments arranged by size: 501 308 286 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 420 867 1095 Size: 420 447 228 Fragments arranged by size: 447 420 228 HaeII r_GCGC'y Cuts at: 0 743 1095 Size: 743 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 43 75 357 389 1095 Size: 43 32 282 32 706 Fragments arranged by size: 706 282 43 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 788 1095 Size: 788 307 MslI CAynn'nnrTG Cuts at: 0 306 1095 Size: 306 789 MspA1I CmG'CkG Cuts at: 0 693 1095 Size: 693 402 PasI CC'CwG_GG Cuts at: 0 923 1095 Size: 923 172 PfoI T'CCnGG_A Cuts at: 0 268 338 1095 Size: 268 70 757 Fragments arranged by size: 757 268 70 PpuMI rG'GwC_Cy Cuts at: 0 501 1095 Size: 501 594 PshAI GACnn'nnGTC Cuts at: 0 800 1095 Size: 800 295 PspOMI G'GGCC_C Cuts at: 0 7 1095 Size: 7 1088 PstI C_TGCA'G Cuts at: 0 641 1095 Size: 641 454 PvuI CG_AT'CG Cuts at: 0 882 1095 Size: 882 213 SacI G_AGCT'C Cuts at: 0 370 1095 Size: 370 725 SfcI C'TryA_G Cuts at: 0 637 1095 Size: 637 458 SmaI CCC'GGG Cuts at: 0 29 448 506 1095 Size: 29 419 58 589 Fragments arranged by size: 589 419 58 29 SmlI C'TyrA_G Cuts at: 0 136 997 1095 Size: 136 861 98 Fragments arranged by size: 861 136 98 StuI AGG'CCT Cuts at: 0 400 1066 1095 Size: 400 666 29 Fragments arranged by size: 666 400 29 StyI C'CwwG_G Cuts at: 0 395 413 419 1095 Size: 395 18 6 676 Fragments arranged by size: 676 395 18 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 178 313 1095 Size: 178 135 782 Fragments arranged by size: 782 178 135 TatI w'GTAC_w Cuts at: 0 128 347 1095 Size: 128 219 748 Fragments arranged by size: 748 219 128 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1011 1095 Size: 1011 84 XhoI C'TCGA_G Cuts at: 0 136 1095 Size: 136 959 XmaI C'CCGG_G Cuts at: 0 27 446 504 1095 Size: 27 419 58 591 Fragments arranged by size: 591 419 58 27 XmnI GAAnn'nnTTC Cuts at: 0 850 1095 Size: 850 245 Enzymes that do cut: AcuI AleI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbsI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BsrBI BstXI BstYI Bsu36I BtgI DrdI EaeI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I MmeI MslI MspA1I PasI PfoI PpuMI PshAI PspOMI PstI PvuI SacI SfcI SmaI SmlI StuI StyI TaqII TatI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI BsaAI BsaXI BsiWI BsmI BsmBI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EagI EarI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI ZraI