################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MflI MluI MroI MseI MunI NcoI NdeI NotI NruI NsiI NspV PacI PflFI PmeI PshBI Psp1406I RcaI RsrII SacI SbfI ScaI SfaNI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp509I Tth111I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 435 GACGTC GACGT^C AccI 1030 GTCGAC GT^[AC][GT]AC AfaI 738 GTAC GT^AC AflIII 387 ACACGT A^C[GA][CT]GT AlwNI 703 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 523 GTGCTC G[AT]GC[AT]^C AviII 455 TGCGCA TGC^GCA BbsI 595 GTCTTC GAAGAC(2/6) BbvCI 27 CCTCAGC CCTCAGC(-5/-2) BpmI 424 CTGGAG CTGGAG(16/14) BpuAI 595 GTCTTC GAAGAC(2/6) BsiHKAI 523 GTGCTC G[AT]GC[AT]^C BspMI 662 ACCTGC ACCTGC(4/8) BssSI 802 CTCGTG CACGAG(-5/-1) DraIII 939 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 479 CGGCCG C^GGCCG EclXI 479 CGGCCG C^GGCCG Eco52I 479 CGGCCG C^GGCCG EcoNI 678 CCTGGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 455 TGCGCA TGC^GCA HincII 1030 GTCGAC GT[CT]^[GA]AC HindII 1030 GTCGAC GT[CT]^[GA]AC KspI 144 CCGCGG CCGC^GG NheI 1261 GCTAGC G^CTAGC NspI 238 ACATGC [GA]CATG^[CT] PaeR7I 907 CTCGAG C^TCGAG PflMI 756 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 835 GACTC GAGTC(4/5) PpuMI 1061 AGGTCCC [GA]G^G[AT]CC[CT] PvuI 789 CGATCG CGAT^CG PvuII 703 CAGCTG CAG^CTG RsaI 738 GTAC GT^AC SacII 144 CCGCGG CCGC^GG SalI 1030 GTCGAC G^TCGAC SexAI 677 ACCTGGT A^CC[AT]GGT SgrAI 116 CACCGGTG C[GA]^CCGG[CT]G SmaI 85 CCCGGG CCC^GGG TfiI 43 GATTC G^A[AT]TC Van91I 756 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 907 CTCGAG C^TCGAG XmaCI 85 CCCGGG C^CCGGG XmaI 85 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AgeI 117 ACCGGT A^CCGGT 1074 ACCGGT ApaI 14 GGGCCC GGGCC^C 82 GGGCCC AvaII 750 GGACC G^G[AT]CC 1062 GGTCC BanII 14 GGGCCC G[GA]GC[CT]^C 82 GGGCCC BbrPI 388 CACGTG CAC^GTG 691 CACGTG BsaAI 388 CACGTG [CT]AC^GT[GA] 691 CACGTG BsaWI 117 ACCGGT [AT]^CCGG[AT] 1074 ACCGGT BseRI 73 GAGGAG GAGGAG(10/8) 469 GAGGAG BsgI 340 CTGCAC GTGCAG(16/14) 121 GTGCAG BsmBI 427 GAGACG CGTCTC(1/5) 586 GAGACG BsrDI 330 CATTGC GCAATG(2/0) 510 CATTGC BssHII 209 GCGCGC G^CGCGC 211 GCGCGC BstXI 236 CCACATGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 395 CCAGCTTCCTGG DpnII 790 GATC ^GATC 831 GATC DrdI 429 GACGCAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 529 GACGCCTTCGTC HinfI 43 GATTC G^A[ACGT]TC 835 GACTC MboI 790 GATC ^GATC 831 GATC NdeII 790 GATC ^GATC 831 GATC PinAI 117 ACCGGT A^CCGGT 1074 ACCGGT PmaCI 388 CACGTG CAC^GTG 691 CACGTG PmlI 388 CACGTG CAC^GTG 691 CACGTG PshAI 588 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1071 GACACCGGTC SapI 273 GCTCTTC GCTCTTC(1/4) 306 GCTCTTC Sau3AI 790 GATC ^GATC 831 GATC SfiI 480 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 555 GGCCAAGATGGCC SmlI 907 CTCGAG C^T[CT][GA]AG 1129 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AcyI AluI AvaI BalI BanI BbeI BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV PstI Sau96I ScrFI SfcI SfoI TaqI TseI Tsp45I TspRI TthHB8I