(Circular) (Six-base) MAPSORT of: pFN21AE5022.seq Check: 6417 from: 1 to: 6013 pFN21AE5022 6013 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2444 3823 278 Size: 53 83 186 936 908 1379 2468 Fragments arranged by size: 2468 1379 936 908 186 83 53 AccI GT'mk_AC Cuts at: 1937 3036 3356 1937 Size: 1099 320 4594 Fragments arranged by size: 4594 1099 320 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2587 4076 5460 5728 1632 Size: 955 1489 1384 268 1917 Fragments arranged by size: 1917 1489 1384 955 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 2392 4912 5889 1203 Size: 1189 2520 977 1327 Fragments arranged by size: 2520 1327 1189 977 AgeI A'CCGG_T Cuts at: 2122 3079 4844 2122 Size: 957 1765 3291 Fragments arranged by size: 3291 1765 957 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 2246 2700 1166 Size: 1080 454 4479 Fragments arranged by size: 4479 1080 454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2532 1184 Size: 1348 4665 AlwNI CAG_nnn'CTG Cuts at: 2713 3756 5328 2713 Size: 1043 1572 3398 Fragments arranged by size: 3398 1572 1043 ApaI G_GGCC'C Cuts at: 2023 2091 2023 Size: 68 5945 ApaLI G'TGCA_C Cuts at: 4070 5226 4070 Size: 1156 4857 ApoI r'AATT_y Cuts at: 1493 3322 3548 3700 5809 1493 Size: 1829 226 152 2109 1697 Fragments arranged by size: 2109 1829 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4567 160 Size: 872 3535 1606 Fragments arranged by size: 3535 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2912 3273 3338 1870 Size: 73 147 822 361 65 4545 Fragments arranged by size: 4545 822 361 147 73 65 BamHI G'GATC_C Cuts at: 3343 3710 3343 Size: 367 5646 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2113 2119 2145 2789 Size: 325 217 627 252 74 6 26 644 Cuts at: 2789 3334 4789 618 Size: 545 1455 1842 Fragments arranged by size: 1842 1455 644 627 545 325 252 217 74 26 6 BanII G_rGCy'C Cuts at: 1391 2023 2091 3332 1391 Size: 632 68 1241 4072 Fragments arranged by size: 4072 1241 632 68 BbeI G_GCGC'C Cuts at: 2043 2117 2149 2043 Size: 74 32 5907 Fragments arranged by size: 5907 74 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2593 928 Size: 971 694 4348 Fragments arranged by size: 4348 971 694 BbvCI CC'TCA_GC Cuts at: 2033 2033 Size: 6013 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4204 4238 1903 Size: 34 2267 34 3678 Fragments arranged by size: 3678 2267 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 1548 Size: 6013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 2067 2492 Size: 107 122 71 631 605 365 30 425 Cuts at: 2492 2567 2621 2879 4624 136 Size: 75 54 258 1745 1525 Fragments arranged by size: 1745 1525 631 605 425 365 258 122 107 75 71 54 30 BglII A'GATC_T Cuts at: 6008 6008 Size: 6013 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 2023 2091 2178 2483 2707 4074 5230 1790 Size: 233 68 87 305 224 1367 1156 2573 Fragments arranged by size: 2573 1367 1156 305 233 224 87 68 BmgBI CAC'GTC Cuts at: 1318 2254 2870 1318 Size: 936 616 4461 Fragments arranged by size: 4461 936 616 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3019 3744 4702 Size: 416 53 83 399 24 1602 725 958 Cuts at: 4702 442 Size: 1753 Fragments arranged by size: 1753 1602 958 725 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3270 1056 Size: 2214 3799 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2857 2857 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 3294 4673 1239 Size: 1211 844 1379 2579 Fragments arranged by size: 2579 1379 1211 844 Bpu10I CC'TnA_GC Cuts at: 2033 2225 3093 2033 Size: 192 868 4953 Fragments arranged by size: 4953 868 192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3119 4138 5003 5301 3119 Size: 1019 865 298 3831 Fragments arranged by size: 3831 1019 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4676 882 Size: 617 3177 2219 Fragments arranged by size: 3177 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2395 2698 3804 493 Size: 711 1191 303 1106 2702 Fragments arranged by size: 2702 1191 1106 711 303 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 2114 2146 2441 Size: 53 83 186 936 507 74 32 295 Cuts at: 2441 2535 3820 4202 4837 275 Size: 94 1285 382 635 1451 Fragments arranged by size: 1451 1285 936 635 507 382 295 186 94 83 74 53 32 BsaWI w'CCGG_w Cuts at: 2122 3079 4446 4844 5118 5265 5676 2122 Size: 957 1367 398 274 147 411 2459 Fragments arranged by size: 2459 1367 957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3292 3322 1178 Size: 30 165 30 1889 30 3869 Fragments arranged by size: 3869 1889 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2489 1186 Size: 907 396 4710 Fragments arranged by size: 4710 907 396 BseYI C'CCAG_C Cuts at: 2706 5216 5712 2706 Size: 2510 496 3007 Fragments arranged by size: 3007 2510 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 2330 1163 Size: 573 411 183 4846 Fragments arranged by size: 4846 573 411 183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2155 2487 2797 3463 4224 5252 Size: 275 186 154 332 310 666 761 1028 Cuts at: 5252 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 666 332 310 275 186 154 BsiHKAI G_wGCw'C Cuts at: 2532 4074 4159 5230 2532 Size: 1542 85 1071 3315 Fragments arranged by size: 3315 1542 1071 85 BsmI GAATG_Cn' Cuts at: 3524 3617 3524 Size: 93 5920 BsmBI CGTCTCn'nnnn_ Cuts at: 2426 2585 2426 Size: 159 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2091 2178 2483 2532 2707 3332 Size: 399 233 68 87 305 49 175 625 Cuts at: 3332 4074 4159 5230 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 625 399 305 233 175 87 85 68 49 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 3902 5632 3902 Size: 1730 4283 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2676 3368 844 Size: 1832 692 3489 Fragments arranged by size: 3489 1832 692 BsrBI CCG'CTC Cuts at: 1394 1823 3900 1394 Size: 429 2077 3507 Fragments arranged by size: 3507 2077 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2334 2514 4508 4682 65 Size: 1183 1086 180 1994 174 1396 Fragments arranged by size: 1994 1396 1183 1086 180 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2116 2122 2142 2519 3079 4657 4844 Size: 110 306 6 20 377 560 1578 187 Cuts at: 4844 1700 Size: 2869 Fragments arranged by size: 2869 1578 560 377 306 187 110 20 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 2214 2216 1925 Size: 289 2 5722 Fragments arranged by size: 5722 289 2 BssSI C'ACGA_G Cuts at: 2807 4073 5085 2807 Size: 1266 1012 3735 Fragments arranged by size: 3735 1266 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6013 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2248 2407 2011 Size: 237 159 5617 Fragments arranged by size: 5617 237 159 BstYI r'GATC_y Cuts at: 1972 3343 3710 4095 4112 5564 5650 5662 6008 Size: 1371 367 385 17 1452 86 12 346 Cuts at: 6008 1972 Size: 1977 Fragments arranged by size: 1977 1452 1371 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 2149 2499 513 Size: 552 939 132 13 350 4027 Fragments arranged by size: 4027 939 552 350 132 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2085 3038 487 Size: 683 56 859 953 3462 Fragments arranged by size: 3462 953 859 683 56 BtsI GCAGTG_nn' Cuts at: 2176 2516 3094 3529 4323 4343 2176 Size: 340 578 435 794 20 3846 Fragments arranged by size: 3846 794 578 435 340 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DraIII CAC_nnn'GTG Cuts at: 2949 2949 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2440 2540 5020 809 Size: 507 1124 100 2480 1802 Fragments arranged by size: 2480 1802 1124 507 100 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2206 2358 Size: 54 1425 50 275 18 183 193 152 Cuts at: 2358 2397 2409 2484 2493 2559 2568 2880 3460 Size: 39 12 75 9 66 9 312 580 Cuts at: 3460 4349 5978 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 580 312 275 193 183 152 75 66 54 50 43 39 18 12 9 9 EagI C'GGCC_G Cuts at: 1537 1812 2484 3460 1537 Size: 275 672 976 4090 Fragments arranged by size: 4090 976 672 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 2285 2318 2426 3014 3943 1353 Size: 717 215 33 108 588 929 3423 Fragments arranged by size: 3423 929 717 588 215 108 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 4974 5120 387 Size: 1064 138 3011 374 146 1280 Fragments arranged by size: 3011 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2587 3294 4076 4673 5460 5728 Size: 393 818 137 707 782 597 787 268 Cuts at: 5728 1239 Size: 1524 Fragments arranged by size: 1524 818 787 782 707 597 393 268 137 EcoNI CCTnn'n_nnAGG Cuts at: 2687 2687 Size: 6013 EcoO109I rG'GnC_Cy Cuts at: 2019 2020 3067 2019 Size: 1 1047 4965 Fragments arranged by size: 4965 1047 1 EcoRI G'AATT_C Cuts at: 3322 3700 3322 Size: 378 5635 FspI TGC'GCA Cuts at: 2462 4519 2462 Size: 2057 3956 HaeII r_GCGC'y Cuts at: 1678 2043 2117 2149 2266 2627 3299 5160 1678 Size: 365 74 32 117 361 672 1861 2531 Fragments arranged by size: 2531 1861 672 365 361 117 74 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3292 3324 4654 4686 4728 Size: 32 135 32 1720 32 1330 32 42 Cuts at: 4728 4760 1373 Size: 32 2626 Fragments arranged by size: 2626 1720 1330 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3037 3357 3603 669 Size: 988 281 1099 320 246 3079 Fragments arranged by size: 3079 1099 988 320 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6013 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KasI G'GCGC_C Cuts at: 2039 2113 2145 2039 Size: 74 32 5907 Fragments arranged by size: 5907 74 32 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5127 5311 1305 Size: 3822 184 2007 Fragments arranged by size: 3822 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2015 2208 2399 2561 2570 Size: 54 1425 343 183 193 191 162 9 Cuts at: 2570 2882 10 Size: 312 3141 Fragments arranged by size: 3141 1425 343 312 193 191 183 162 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2700 2763 2936 3971 4330 4489 Size: 648 1080 454 63 173 1035 359 159 Cuts at: 4489 518 Size: 2042 Fragments arranged by size: 2042 1080 1035 648 454 359 173 159 63 MspA1I CmG'CkG Cuts at: 1606 2037 2151 2489 2710 2752 3429 4106 5254 Size: 431 114 338 221 42 677 677 1148 Cuts at: 5254 5499 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 677 431 338 245 221 114 42 NaeI GCC'GGC Cuts at: 2118 2144 2521 2118 Size: 26 377 5610 Fragments arranged by size: 5610 377 26 NarI GG'CG_CC Cuts at: 2040 2114 2146 2040 Size: 74 32 5907 Fragments arranged by size: 5907 74 32 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4522 Fragments arranged by size: 4522 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NgoMIV G'CCGG_C Cuts at: 2116 2142 2519 2116 Size: 26 377 5610 Fragments arranged by size: 5610 377 26 NheI G'CTAG_C Cuts at: 1052 3266 1052 Size: 2214 3799 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 2247 3371 4916 1132 Size: 97 1018 1124 1545 2229 Fragments arranged by size: 2229 1545 1124 1018 97 PasI CC'CwG_GG Cuts at: 1348 3071 1348 Size: 1723 4290 PciI A'CATG_T Cuts at: 4912 4912 Size: 6013 PflMI CCAn_nnn'nTGG Cuts at: 1108 2767 1108 Size: 1659 4354 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PmlI CAC'GTG Cuts at: 2395 2698 2395 Size: 303 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5595 5627 4082 Size: 32 1481 32 4468 Fragments arranged by size: 4468 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3067 3067 Size: 6013 PshAI GACnn'nnGTC Cuts at: 1613 2597 3080 1613 Size: 984 483 4546 Fragments arranged by size: 4546 984 483 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PspOMI G'GGCC_C Cuts at: 2019 2087 2019 Size: 68 5945 PstI C_TGCA'G Cuts at: 830 2511 2718 3089 3365 830 Size: 1681 207 371 276 3478 Fragments arranged by size: 3478 1681 371 276 207 PvuI CG_AT'CG Cuts at: 2001 2797 2001 Size: 796 5217 PvuII CAG'CTG Cuts at: 2710 2710 Size: 6013 SacII CC_GC'GG Cuts at: 2152 2152 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3035 3355 1936 Size: 1099 320 4594 Fragments arranged by size: 4594 1099 320 SapI GCTCTTCn'nnn_ Cuts at: 2285 2318 2285 Size: 33 5980 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 4261 5973 4261 Size: 1712 4301 SexAI A'CCwGG_T Cuts at: 2682 2682 Size: 6013 SfcI C'TryA_G Cuts at: 826 1045 2507 2714 3085 3361 4496 5177 5368 Size: 219 1462 207 371 276 1135 681 191 Cuts at: 5368 826 Size: 1471 Fragments arranged by size: 1471 1462 1135 681 371 276 219 207 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2492 2567 2492 Size: 75 5938 SfoI GGC'GCC Cuts at: 2041 2115 2147 2041 Size: 74 32 5907 Fragments arranged by size: 5907 74 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 2122 1700 Size: 422 5591 SmaI CCC'GGG Cuts at: 1872 2092 3340 1872 Size: 220 1248 4545 Fragments arranged by size: 4545 1248 220 SmlI C'TyrA_G Cuts at: 820 1017 1943 2912 3134 4117 5018 5280 820 Size: 197 926 969 222 983 901 262 1553 Fragments arranged by size: 1553 983 969 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 3937 5 Size: 47 3885 2081 Fragments arranged by size: 3885 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6013 StyI C'CwwG_G Cuts at: 513 1065 2004 3452 513 Size: 552 939 1448 3074 Fragments arranged by size: 3074 1448 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2192 2537 4040 4057 4210 4395 4840 Size: 1255 612 345 1503 17 153 185 445 Cuts at: 4840 325 Size: 1498 Fragments arranged by size: 1503 1498 1255 612 445 345 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2742 4259 5971 Size: 274 80 33 51 1443 765 1517 1712 Cuts at: 5971 96 Size: 138 Fragments arranged by size: 1712 1517 1443 765 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5973 6005 5973 Size: 32 5981 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6013 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XhoI C'TCGA_G Cuts at: 1943 2912 1943 Size: 969 5044 XmaI C'CCGG_G Cuts at: 1870 2090 3338 1870 Size: 220 1248 4545 Fragments arranged by size: 4545 1248 220 XmnI GAAnn'nnTTC Cuts at: 4142 4142 Size: 6013 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2442 3821 276 Size: 53 83 186 936 908 1379 2468 Fragments arranged by size: 2468 1379 936 908 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI SrfI SwaI XcmI