(Linear) (Six-base) MAPSORT of: KIEE5022.seq Check: 4132 from: 1 to: 1308 KIEE5022.orf With 182 enzymes: SgfI * December 5, 2014 12:58 .. AatII G_ACGT'C Cuts at: 0 439 1308 Size: 439 869 AccI GT'mk_AC Cuts at: 0 1031 1308 Size: 1031 277 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 582 1308 Size: 582 726 AflIII A'CryG_T Cuts at: 0 387 1308 Size: 387 921 AgeI A'CCGG_T Cuts at: 0 117 1074 1308 Size: 117 957 234 Fragments arranged by size: 957 234 117 AleI CACnn'nnGTG Cuts at: 0 241 695 1308 Size: 241 454 613 Fragments arranged by size: 613 454 241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 527 1308 Size: 527 781 AlwNI CAG_nnn'CTG Cuts at: 0 708 1308 Size: 708 600 ApaI G_GGCC'C Cuts at: 0 18 86 1308 Size: 18 68 1222 Fragments arranged by size: 1222 68 18 AvaI C'yCGr_G Cuts at: 0 85 907 1268 1308 Size: 85 822 361 40 Fragments arranged by size: 822 361 85 40 BanI G'GyrC_C Cuts at: 0 34 108 114 140 784 1308 Size: 34 74 6 26 644 524 Fragments arranged by size: 644 524 74 34 26 6 BanII G_rGCy'C Cuts at: 0 18 86 1308 Size: 18 68 1222 Fragments arranged by size: 1222 68 18 BbeI G_GCGC'C Cuts at: 0 38 112 144 1308 Size: 38 74 32 1164 Fragments arranged by size: 1164 74 38 32 BbsI GAAGACnn'nnnn_ Cuts at: 0 588 1308 Size: 588 720 BbvCI CC'TCA_GC Cuts at: 0 28 1308 Size: 28 1280 BglI GCCn_nnn'nGGC Cuts at: 0 32 62 487 562 616 874 1308 Size: 32 30 425 75 54 258 434 Fragments arranged by size: 434 425 258 75 54 32 30 Bme1580I G_kGCm'C Cuts at: 0 18 86 173 478 702 1308 Size: 18 68 87 305 224 606 Fragments arranged by size: 606 305 224 87 68 18 BmgBI CAC'GTC Cuts at: 0 249 865 1308 Size: 249 616 443 Fragments arranged by size: 616 443 249 BmrI ACTGGGnnnn_n' Cuts at: 0 1014 1308 Size: 1014 294 BmtI G_CTAG'C Cuts at: 0 1265 1308 Size: 1265 43 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 852 1308 Size: 852 456 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 445 1308 Size: 445 863 Bpu10I CC'TnA_GC Cuts at: 0 28 220 1088 1308 Size: 28 192 868 220 Fragments arranged by size: 868 220 192 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1114 1308 Size: 1114 194 BsaAI yAC'GTr Cuts at: 0 390 693 1308 Size: 390 303 615 Fragments arranged by size: 615 390 303 BsaHI Gr'CG_yC Cuts at: 0 35 109 141 436 530 1308 Size: 35 74 32 295 94 778 Fragments arranged by size: 778 295 94 74 35 32 BsaWI w'CCGG_w Cuts at: 0 117 1074 1308 Size: 117 957 234 Fragments arranged by size: 957 234 117 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1287 1308 Size: 1287 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 88 484 1308 Size: 88 396 824 Fragments arranged by size: 824 396 88 BseYI C'CCAG_C Cuts at: 0 701 1308 Size: 701 607 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 325 1308 Size: 142 183 983 Fragments arranged by size: 983 183 142 BsiEI CG_ry'CG Cuts at: 0 150 482 792 1308 Size: 150 332 310 516 Fragments arranged by size: 516 332 310 150 BsiHKAI G_wGCw'C Cuts at: 0 527 1308 Size: 527 781 BsmBI CGTCTCn'nnnn_ Cuts at: 0 421 580 1308 Size: 421 159 728 Fragments arranged by size: 728 421 159 Bsp1286I G_dGCh'C Cuts at: 0 18 86 173 478 527 702 1308 Size: 18 68 87 305 49 175 606 Fragments arranged by size: 606 305 175 87 68 49 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 671 1308 Size: 671 637 BsrDI GCAATG_nn' Cuts at: 0 329 509 1308 Size: 329 180 799 Fragments arranged by size: 799 329 180 BsrFI r'CCGG_y Cuts at: 0 111 117 137 514 1074 1308 Size: 111 6 20 377 560 234 Fragments arranged by size: 560 377 234 111 20 6 BssHII G'CGCG_C Cuts at: 0 209 211 1308 Size: 209 2 1097 Fragments arranged by size: 1097 209 2 BssSI C'ACGA_G Cuts at: 0 802 1308 Size: 802 506 BstXI CCAn_nnnn'nTGG Cuts at: 0 243 402 1308 Size: 243 159 906 Fragments arranged by size: 906 243 159 BtgI C'CryG_G Cuts at: 0 131 144 494 1308 Size: 131 13 350 814 Fragments arranged by size: 814 350 131 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 80 1033 1308 Size: 80 953 275 Fragments arranged by size: 953 275 80 BtsI GCAGTG_nn' Cuts at: 0 171 511 1089 1308 Size: 171 340 578 219 Fragments arranged by size: 578 340 219 171 DraIII CAC_nnn'GTG Cuts at: 0 944 1308 Size: 944 364 DrdI GACnn_nn'nnGTC Cuts at: 0 435 535 1308 Size: 435 100 773 Fragments arranged by size: 773 435 100 EaeI y'GGCC_r Cuts at: 0 8 201 353 392 404 479 488 554 Size: 8 193 152 39 12 75 9 66 Cuts at: 554 563 875 1308 Size: 9 312 433 Fragments arranged by size: 433 312 193 152 75 66 39 12 9 9 8 EagI C'GGCC_G Cuts at: 0 479 1308 Size: 479 829 EarI CTCTTCn'nnn_ Cuts at: 0 65 280 313 421 1009 1308 Size: 65 215 33 108 588 299 Fragments arranged by size: 588 299 215 108 65 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 445 582 1308 Size: 445 137 726 Fragments arranged by size: 726 445 137 EcoNI CCTnn'n_nnAGG Cuts at: 0 682 1308 Size: 682 626 EcoO109I rG'GnC_Cy Cuts at: 0 14 15 1062 1308 Size: 14 1 1047 246 Fragments arranged by size: 1047 246 14 1 FspI TGC'GCA Cuts at: 0 457 1308 Size: 457 851 HaeII r_GCGC'y Cuts at: 0 38 112 144 261 622 1294 1308 Size: 38 74 32 117 361 672 14 Fragments arranged by size: 672 361 117 74 38 32 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1287 1308 Size: 1287 21 HincII GTy'rAC Cuts at: 0 1032 1308 Size: 1032 276 KasI G'GCGC_C Cuts at: 0 34 108 140 1308 Size: 34 74 32 1168 Fragments arranged by size: 1168 74 34 32 MscI TGG'CCA Cuts at: 0 10 203 394 556 565 877 1308 Size: 10 193 191 162 9 312 431 Fragments arranged by size: 431 312 193 191 162 10 9 MslI CAynn'nnrTG Cuts at: 0 241 695 758 931 1308 Size: 241 454 63 173 377 Fragments arranged by size: 454 377 241 173 63 MspA1I CmG'CkG Cuts at: 0 32 146 484 705 747 1308 Size: 32 114 338 221 42 561 Fragments arranged by size: 561 338 221 114 42 32 NaeI GCC'GGC Cuts at: 0 113 139 516 1308 Size: 113 26 377 792 Fragments arranged by size: 792 377 113 26 NarI GG'CG_CC Cuts at: 0 35 109 141 1308 Size: 35 74 32 1167 Fragments arranged by size: 1167 74 35 32 NgoMIV G'CCGG_C Cuts at: 0 111 137 514 1308 Size: 111 26 377 794 Fragments arranged by size: 794 377 111 26 NheI G'CTAG_C Cuts at: 0 1261 1308 Size: 1261 47 NspI r_CATG'y Cuts at: 0 242 1308 Size: 242 1066 PasI CC'CwG_GG Cuts at: 0 1066 1308 Size: 1066 242 PflMI CCAn_nnn'nTGG Cuts at: 0 762 1308 Size: 762 546 PmlI CAC'GTG Cuts at: 0 390 693 1308 Size: 390 303 615 Fragments arranged by size: 615 390 303 PpuMI rG'GwC_Cy Cuts at: 0 1062 1308 Size: 1062 246 PshAI GACnn'nnGTC Cuts at: 0 592 1075 1308 Size: 592 483 233 Fragments arranged by size: 592 483 233 PspOMI G'GGCC_C Cuts at: 0 14 82 1308 Size: 14 68 1226 Fragments arranged by size: 1226 68 14 PstI C_TGCA'G Cuts at: 0 506 713 1084 1308 Size: 506 207 371 224 Fragments arranged by size: 506 371 224 207 PvuI CG_AT'CG Cuts at: 0 792 1308 Size: 792 516 PvuII CAG'CTG Cuts at: 0 705 1308 Size: 705 603 SacII CC_GC'GG Cuts at: 0 147 1308 Size: 147 1161 SalI G'TCGA_C Cuts at: 0 1030 1308 Size: 1030 278 SapI GCTCTTCn'nnn_ Cuts at: 0 280 313 1308 Size: 280 33 995 Fragments arranged by size: 995 280 33 SexAI A'CCwGG_T Cuts at: 0 677 1308 Size: 677 631 SfcI C'TryA_G Cuts at: 0 502 709 1080 1308 Size: 502 207 371 228 Fragments arranged by size: 502 371 228 207 SfiI GGCCn_nnn'nGGCC Cuts at: 0 487 562 1308 Size: 487 75 746 Fragments arranged by size: 746 487 75 SfoI GGC'GCC Cuts at: 0 36 110 142 1308 Size: 36 74 32 1166 Fragments arranged by size: 1166 74 36 32 SgrAI Cr'CCGG_yG Cuts at: 0 117 1308 Size: 117 1191 SmaI CCC'GGG Cuts at: 0 87 1308 Size: 87 1221 SmlI C'TyrA_G Cuts at: 0 907 1129 1308 Size: 907 222 179 Fragments arranged by size: 907 222 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 187 532 1308 Size: 187 345 776 Fragments arranged by size: 776 345 187 TatI w'GTAC_w Cuts at: 0 737 1308 Size: 737 571 XhoI C'TCGA_G Cuts at: 0 907 1308 Size: 907 401 XmaI C'CCGG_G Cuts at: 0 85 1308 Size: 85 1223 ZraI GAC'GTC Cuts at: 0 437 1308 Size: 437 871 Enzymes that do cut: AatII AccI AcuI AflIII AgeI AleI AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssHII BssSI BstXI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI PflMI PmlI PpuMI PshAI PspOMI PstI PvuI PvuII SacII SalI SapI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI TaqII TatI XhoI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII BlpI BsaI BsaBI BsiWI BsmI BspEI BspHI BsrBI BsrGI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NotI NruI NsiI PacI PciI PfoI PmeI PpiI PsiI PspXI PsrI RsrII SacI SanDI SbfI ScaI SgfI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TstI Tth111I XbaI XcmI XmnI