################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BciVI BclI BfaI BfrI BglII BlnI BsaBI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI MaeI MamI MfeI MluI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI SacI SalI ScaI SexAI SfuI SmlI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccIII 112 TCCGGA T^CCGGA AcsI 472 AAATTC [GA]^AATT[CT] AflIII 863 ACACGT A^C[GA][CT]GT Alw44I 1608 GTGCAC G^TGCAC AocI 791 CCTCAGG CC^T[ACGT]AGG ApaLI 1608 GTGCAC G^TGCAC ApoI 472 AAATTC [GA]^AATT[CT] BbvCI 267 CCTCAGC CCTCAGC(-5/-2) BcgI 539 GCAGCGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BseAI 112 TCCGGA T^CCGGA BspEI 112 TCCGGA T^CCGGA BspMI 1664 ACCTGC ACCTGC(4/8) BsrBI 1164 GAGCGG CCGCTC(-3/-3) BssHII 160 GCGCGC G^CGCGC BssSI 1041 CACGAG CACGAG(-5/-1) Bsu36I 791 CCTCAGG CC^T[ACGT]AGG CpoI 114 CGGACCG CG^G[AT]CCG DraIII 1186 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 791 CCTCAGG CC^T[ACGT]AGG HindIII 208 AAGCTT A^AGCTT HinfI 962 GAGTC G^A[ACGT]TC MroI 112 TCCGGA T^CCGGA PleI 962 GAGTC GAGTC(4/5) PshAI 1075 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 114 CGGACCG CG^G[AT]CCG SapI 1170 GAAGAGC GCTCTTC(1/4) SbfI 123 CCTGCAGG CCTGCA^GG SfiI 364 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1589 CGCCGGTG C[GA]^CCGG[CT]G Sse8387I 123 CCTGCAGG CCTGCA^GG Tsp509I 473 AATT ^AATT XcmI 1218 CCAAGCCGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 798 GTAC GT^AC 1656 GTAC Aor51HI 381 AGCGCT AGC^GCT 731 AGCGCT BlpI 886 GCTGAGC GC^T[ACGT]AGC 1212 GCTCAGC Bpu1102I 886 GCTGAGC GC^T[ACGT]AGC 1212 GCTCAGC BsaWI 112 TCCGGA [AT]^CCGG[AT] 1518 TCCGGT BsgI 778 CTGCAC GTGCAG(16/14) 1570 CTGCAC BsmBI 636 CGTCTC CGTCTC(1/5) 922 CGTCTC BsrFI 293 GCCGGT [GA]^CCGG[CT] 1590 GCCGGT BstAPI 861 GCACACGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1185 GCACCATGTGC CelII 886 GCTGAGC GC^T[ACGT]AGC 1212 GCTCAGC Cfr10I 293 GCCGGT [GA]^CCGG[CT] 1590 GCCGGT Eco47III 381 AGCGCT AGC^GCT 731 AGCGCT NcoI 900 CCATGG C^CATGG 1528 CCATGG PflMI 834 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 895 CCACTCCATGG PpuMI 1502 GGGTCCT [GA]G^G[AT]CC[CT] 1550 GGGACCC RsaI 798 GTAC GT^AC 1656 GTAC StuI 788 AGGCCT AGG^CCT 1700 AGGCCT Van91I 834 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 895 CCACTCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI Ksp632I KspI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PmaCI PmlI PstI PvuII SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII XmaCI XmaI