(Circular) (Six-base) MAPSORT of: pFN21AB7802.seq Check: 2430 from: 1 to: 6475 pFN21AB7802 6475 bp With 182 enzymes: SgfI * March 28, 2012 12:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3678 3678 Size: 6475 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4285 278 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3818 1937 Size: 1881 4594 Acc65I G'GTAC_C Cuts at: 3796 3796 Size: 6475 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3533 4538 5922 6190 1632 Size: 1901 1005 1384 268 1917 Fragments arranged by size: 1917 1901 1384 1005 268 AfeI AGC'GCT Cuts at: 2388 2738 2388 Size: 350 6125 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6278 AflIII A'CryG_T Cuts at: 1203 2868 5374 6351 1203 Size: 1665 2506 977 1327 Fragments arranged by size: 2506 1665 1327 977 AgeI A'CCGG_T Cuts at: 5306 5306 Size: 6475 AhdI GACnn_n'nnGTC Cuts at: 5204 5204 Size: 6475 AleI CACnn'nnGTG Cuts at: 1166 2708 1166 Size: 1542 4933 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3773 1184 Size: 2589 3886 AlwNI CAG_nnn'CTG Cuts at: 2416 2440 3164 3679 4218 5790 2416 Size: 24 724 515 539 1572 3101 Fragments arranged by size: 3101 1572 724 539 515 24 ApaI G_GGCC'C Cuts at: 2839 2994 3692 3744 2839 Size: 155 698 52 5570 Fragments arranged by size: 5570 698 155 52 ApaLI G'TGCA_C Cuts at: 3613 4532 5688 3613 Size: 919 1156 4400 Fragments arranged by size: 4400 1156 919 ApoI r'AATT_y Cuts at: 1493 2477 3784 4010 4162 6271 1493 Size: 984 1307 226 152 2109 1697 Fragments arranged by size: 2109 1697 1307 984 226 152 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 2093 2684 2861 3182 3298 3409 3479 Size: 73 150 591 177 321 116 111 70 Cuts at: 3479 3551 3736 3800 1870 Size: 72 185 64 4545 Fragments arranged by size: 4545 591 321 185 177 150 116 111 73 72 70 64 BamHI G'GATC_C Cuts at: 3051 3448 3520 3805 4172 3051 Size: 397 72 285 367 5354 Fragments arranged by size: 5354 397 367 285 72 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2110 2231 2317 2710 Size: 325 217 627 279 44 121 86 393 Cuts at: 2710 2826 3544 3623 3749 3796 5251 618 Size: 116 718 79 126 47 1455 1842 Fragments arranged by size: 1842 1455 718 627 393 325 279 217 126 121 116 86 79 47 44 BanII G_rGCy'C Cuts at: 1391 2839 2994 3182 3312 3405 3692 3744 3794 Size: 1448 155 188 130 93 287 52 50 Cuts at: 3794 1391 Size: 4072 Fragments arranged by size: 4072 1448 287 188 155 130 93 52 50 BbeI G_GCGC'C Cuts at: 2070 2114 2235 2321 2830 3548 2070 Size: 44 121 86 509 718 4997 Fragments arranged by size: 4997 718 509 121 86 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2395 2542 2914 928 Size: 971 496 147 372 4489 Fragments arranged by size: 4489 971 496 372 147 BbvCI CC'TCA_GC Cuts at: 2273 2273 Size: 6475 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2533 2567 4666 4700 1903 Size: 34 596 34 2099 34 3678 Fragments arranged by size: 3678 2099 596 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4367 5577 4367 Size: 1210 5265 BclI T'GATC_A Cuts at: 1548 1548 Size: 6475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2329 2376 Size: 107 122 71 631 605 337 320 47 Cuts at: 2376 2888 3161 3275 5086 136 Size: 512 273 114 1811 1525 Fragments arranged by size: 1811 1525 631 605 512 337 320 273 122 114 107 71 47 BglII A'GATC_T Cuts at: 6470 6470 Size: 6475 BlpI GC'TnA_GC Cuts at: 2892 3218 3892 2892 Size: 326 674 5475 Fragments arranged by size: 5475 674 326 Bme1580I G_kGCm'C Cuts at: 1790 2715 2839 2868 2994 3617 3692 3744 3752 Size: 925 124 29 126 623 75 52 8 Cuts at: 3752 4536 5692 1790 Size: 784 1156 2573 Fragments arranged by size: 2573 1156 925 784 623 126 124 75 52 29 8 BmgBI CAC'GTC Cuts at: 1318 2788 1318 Size: 1470 5005 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2606 2832 4206 Size: 416 53 83 399 24 1189 226 1374 Cuts at: 4206 5164 442 Size: 958 1753 Fragments arranged by size: 1753 1374 1189 958 416 399 226 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2518 3469 3505 2518 Size: 951 36 5488 Fragments arranged by size: 5488 951 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2224 2609 3439 5135 1239 Size: 974 11 385 830 1696 2579 Fragments arranged by size: 2579 1696 974 830 385 11 Bpu10I CC'TnA_GC Cuts at: 2273 2273 Size: 6475 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4600 5465 5763 4600 Size: 865 298 5312 Fragments arranged by size: 5312 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2398 2522 2945 5138 882 Size: 617 899 124 423 2193 2219 Fragments arranged by size: 2219 2193 899 617 423 124 BsaAI yAC'GTr Cuts at: 493 1204 2818 3067 3214 4266 493 Size: 711 1614 249 147 1052 2702 Fragments arranged by size: 2702 1614 1052 711 249 147 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2111 2232 2318 Size: 53 83 186 936 534 44 121 86 Cuts at: 2318 2609 2615 2827 3042 3545 4282 4664 5299 Size: 291 6 212 215 503 737 382 635 Cuts at: 5299 275 Size: 1451 Fragments arranged by size: 1451 936 737 635 534 503 382 291 215 212 186 121 86 83 53 44 6 BsaWI w'CCGG_w Cuts at: 2117 3523 4908 5306 5580 5727 6138 2117 Size: 1406 1385 398 274 147 411 2454 Fragments arranged by size: 2454 1406 1385 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6250 Fragments arranged by size: 6250 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2635 2644 2647 2710 3299 1186 Size: 1449 9 3 63 589 4362 Fragments arranged by size: 4362 1449 589 63 9 3 BseYI C'CCAG_C Cuts at: 2311 3164 3352 3649 3679 5678 6174 2311 Size: 853 188 297 30 1999 496 2612 Fragments arranged by size: 2612 1999 853 496 297 188 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2768 3560 1163 Size: 573 1032 792 4078 Fragments arranged by size: 4078 1032 792 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3925 4686 5714 1540 Size: 275 186 1924 761 1028 2301 Fragments arranged by size: 2301 1924 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3192 3219 3617 4536 4621 5692 3192 Size: 27 398 919 85 1071 3975 Fragments arranged by size: 3975 1071 919 398 85 27 BsmI GAATG_Cn' Cuts at: 3986 4079 3986 Size: 93 6382 BsmBI CGTCTCn'nnnn_ Cuts at: 2647 2933 2647 Size: 286 6189 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2715 2839 2868 2994 3182 3192 3219 Size: 399 925 124 29 126 188 10 27 Cuts at: 3219 3312 3405 3617 3692 3744 3752 3794 4536 Size: 93 93 212 75 52 8 42 742 Cuts at: 4536 4621 5692 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 925 742 399 212 188 126 124 93 93 85 75 52 42 29 27 10 8 BspEI T'CCGG_A Cuts at: 2117 6138 2117 Size: 4021 2454 BspHI T'CATG_A Cuts at: 4364 6094 4364 Size: 1730 4745 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3678 3830 844 Size: 2834 152 3489 Fragments arranged by size: 3489 2834 152 BsrBI CCG'CTC Cuts at: 1394 1823 3171 4362 1394 Size: 429 1348 1191 3507 Fragments arranged by size: 3507 1348 1191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4970 5144 65 Size: 1183 3722 174 1396 Fragments arranged by size: 3722 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2298 3595 5119 5306 1700 Size: 110 488 1297 1524 187 2869 Fragments arranged by size: 2869 1524 1297 488 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 2165 1925 Size: 240 6235 BssSI C'ACGA_G Cuts at: 3046 4535 5547 3046 Size: 1489 1012 3974 Fragments arranged by size: 3974 1489 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2872 3196 794 Size: 2078 324 4073 Fragments arranged by size: 4073 2078 324 BstBI TT'CG_AA Cuts at: 5279 5279 Size: 6475 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6475 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2512 2822 2248 Size: 264 310 5901 Fragments arranged by size: 5901 310 264 BstYI r'GATC_y Cuts at: 1972 3051 3448 3520 3805 4172 4557 4574 6026 Size: 1079 397 72 285 367 385 17 1452 Cuts at: 6026 6112 6124 6470 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1079 397 385 367 346 285 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 2797 2797 Size: 6475 BtgI C'CryG_G Cuts at: 513 1065 2004 2062 2340 2374 2905 3031 3533 Size: 552 939 58 278 34 531 126 502 Cuts at: 3533 3642 3744 3761 513 Size: 109 102 17 3227 Fragments arranged by size: 3227 939 552 531 502 278 126 109 102 58 34 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5736 Fragments arranged by size: 5736 683 56 BtsI GCAGTG_nn' Cuts at: 2032 3991 4785 4805 2032 Size: 1959 794 20 3702 Fragments arranged by size: 3702 1959 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DraIII CAC_nnn'GTG Cuts at: 3196 3196 Size: 6475 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5482 809 Size: 507 4166 1802 Fragments arranged by size: 4166 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 3153 3235 Size: 54 1425 50 275 18 378 945 82 Cuts at: 3235 3465 3530 3922 4811 6440 8 Size: 230 65 392 889 1629 43 Fragments arranged by size: 1629 1425 945 889 392 378 275 230 82 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3922 1537 Size: 275 2110 4090 Fragments arranged by size: 4090 2110 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2653 2665 2671 3170 4405 1353 Size: 1300 12 6 499 1235 3423 Fragments arranged by size: 3423 1300 1235 499 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2049 2279 3044 5062 5436 5582 Size: 1064 138 460 230 765 2018 374 146 Cuts at: 5582 387 Size: 1280 Fragments arranged by size: 2018 1280 1064 765 460 374 230 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2224 2609 3439 3533 4538 5135 Size: 393 581 11 385 830 94 1005 597 Cuts at: 5135 5922 6190 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1005 830 787 597 581 393 385 268 94 11 EcoNI CCTnn'n_nnAGG Cuts at: 2705 2729 3515 2705 Size: 24 786 5665 Fragments arranged by size: 5665 786 24 EcoO109I rG'GnC_Cy Cuts at: 2990 2991 3367 3508 3538 3556 3607 3689 3740 Size: 1 376 141 30 18 51 82 51 Cuts at: 3740 2990 Size: 5725 Fragments arranged by size: 5725 376 141 82 51 51 30 18 1 EcoRI G'AATT_C Cuts at: 3784 4162 3784 Size: 378 6097 FspI TGC'GCA Cuts at: 4981 4981 Size: 6475 HaeII r_GCGC'y Cuts at: 1678 2070 2114 2235 2321 2390 2740 2830 3548 Size: 392 44 121 86 69 350 90 718 Cuts at: 3548 5622 1678 Size: 2074 2531 Fragments arranged by size: 2531 2074 718 392 350 121 90 86 69 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2412 2444 2901 2933 3275 Size: 32 135 32 840 32 457 32 342 Cuts at: 3275 3307 3667 3699 5116 5148 5190 5222 1373 Size: 32 360 32 1417 32 42 32 2626 Fragments arranged by size: 2626 1417 840 457 360 342 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3819 4065 669 Size: 988 281 1881 246 3079 Fragments arranged by size: 3079 1881 988 281 246 HindIII A'AGCT_T Cuts at: 748 2213 748 Size: 1465 5010 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KasI G'GCGC_C Cuts at: 2066 2110 2231 2317 2826 3544 2066 Size: 44 121 86 509 718 4997 Fragments arranged by size: 4997 718 509 121 86 44 KpnI G_GTAC'C Cuts at: 3800 3800 Size: 6475 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3353 5589 5773 1305 Size: 2048 2236 184 2007 Fragments arranged by size: 2236 2048 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 3155 3237 10 Size: 54 1425 343 378 945 82 3248 Fragments arranged by size: 3248 1425 945 378 343 82 54 MslI CAynn'nnrTG Cuts at: 518 1166 2708 2816 3395 4433 4792 4951 518 Size: 648 1542 108 579 1038 359 159 2042 Fragments arranged by size: 2042 1542 1038 648 579 359 159 108 MspA1I CmG'CkG Cuts at: 1606 2064 2224 2248 2342 2354 2376 3015 3356 Size: 458 160 24 94 12 22 639 341 Cuts at: 3356 3644 3746 3763 3891 4568 5716 5961 1606 Size: 288 102 17 128 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 639 458 341 288 245 160 128 102 94 24 22 17 12 NarI GG'CG_CC Cuts at: 2067 2111 2232 2318 2827 3545 2067 Size: 44 121 86 509 718 4997 Fragments arranged by size: 4997 718 509 121 86 44 NcoI C'CATG_G Cuts at: 513 1065 2004 2905 3533 513 Size: 552 939 901 628 3455 Fragments arranged by size: 3455 939 901 628 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3922 3922 Size: 6475 NspI r_CATG'y Cuts at: 1132 1229 3833 5378 1132 Size: 97 2604 1545 2229 Fragments arranged by size: 2604 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2079 2325 2567 2845 3379 3721 1348 Size: 731 246 242 278 534 342 4102 Fragments arranged by size: 4102 731 534 342 278 246 242 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 2845 2906 1108 Size: 1737 61 4677 Fragments arranged by size: 4677 1737 61 PfoI T'CCnGG_A Cuts at: 2153 2730 3699 2153 Size: 577 969 4929 Fragments arranged by size: 4929 969 577 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PmlI CAC'GTG Cuts at: 2818 3067 3214 2818 Size: 249 147 6079 Fragments arranged by size: 6079 249 147 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4544 4576 6057 6089 4544 Size: 32 1481 32 4930 Fragments arranged by size: 4930 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3508 3556 3508 Size: 48 6427 PshAI GACnn'nnGTC Cuts at: 1613 3084 1613 Size: 1471 5004 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PspOMI G'GGCC_C Cuts at: 2835 2990 3688 3740 2835 Size: 155 698 52 5570 Fragments arranged by size: 5570 698 155 52 PstI C_TGCA'G Cuts at: 830 2133 3120 3147 3675 3827 830 Size: 1303 987 27 528 152 3478 Fragments arranged by size: 3478 1303 987 528 152 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 PvuII CAG'CTG Cuts at: 2248 2354 3015 2248 Size: 106 661 5708 Fragments arranged by size: 5708 661 106 RsrII CG'GwC_CG Cuts at: 2120 2120 Size: 6475 SacII CC_GC'GG Cuts at: 2065 2343 2377 3645 3747 3764 2065 Size: 278 34 1268 102 17 4776 Fragments arranged by size: 4776 1268 278 102 34 17 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SanDI GG'GwC_CC Cuts at: 3556 3556 Size: 6475 SapI GCTCTTCn'nnn_ Cuts at: 3170 3170 Size: 6475 SbfI CC_TGCA'GG Cuts at: 2133 3827 2133 Size: 1694 4781 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SfcI C'TryA_G Cuts at: 826 1045 2129 3116 3143 3671 3823 4958 5639 Size: 219 1084 987 27 528 152 1135 681 Cuts at: 5639 5830 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1084 987 681 528 219 191 152 27 SfiI GGCCn_nnn'nGGCC Cuts at: 2376 2376 Size: 6475 SfoI GGC'GCC Cuts at: 2068 2112 2233 2319 2828 3546 2068 Size: 44 121 86 509 718 4997 Fragments arranged by size: 4997 718 509 121 86 44 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 3595 1700 Size: 1895 4580 SmaI CCC'GGG Cuts at: 1872 2095 3184 3300 3553 3738 3802 1872 Size: 223 1089 116 253 185 64 4545 Fragments arranged by size: 4545 1089 253 223 185 116 64 SmlI C'TyrA_G Cuts at: 820 1017 1943 4579 5480 5742 820 Size: 197 926 2636 901 262 1553 Fragments arranged by size: 2636 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4266 493 Size: 3773 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6475 SphI G_CATG'C Cuts at: 1132 3833 1132 Size: 2701 3774 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 2795 3707 1517 Size: 1278 912 4285 Fragments arranged by size: 4285 1278 912 StyI C'CwwG_G Cuts at: 513 1065 2004 2706 2905 3533 3914 513 Size: 552 939 702 199 628 381 3074 Fragments arranged by size: 3074 939 702 628 552 381 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4502 4519 4672 4857 5302 325 Size: 1255 2922 17 153 185 445 1498 Fragments arranged by size: 2922 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4721 6433 96 Size: 274 80 33 51 1443 2744 1712 138 Fragments arranged by size: 2744 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6435 6467 6435 Size: 32 6443 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6475 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3230 3230 Size: 6475 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6475 XmaI C'CCGG_G Cuts at: 1870 2093 3182 3298 3551 3736 3800 1870 Size: 223 1089 116 253 185 64 4545 Fragments arranged by size: 4545 1089 253 223 185 116 64 XmnI GAAnn'nnTTC Cuts at: 4604 4604 Size: 6475 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4283 276 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI SacI SexAI SrfI SwaI