(Linear) (Six-base) MAPSORT of: KIBB7802.seq Check: 8206 from: 1 to: 1770 KIBB7802.orf With 182 enzymes: SgfI * March 22, 2012 15:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1673 1770 Size: 1673 97 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1528 1770 Size: 1528 242 AfeI AGC'GCT Cuts at: 0 383 733 1770 Size: 383 350 1037 Fragments arranged by size: 1037 383 350 AflIII A'CryG_T Cuts at: 0 863 1770 Size: 863 907 AleI CACnn'nnGTG Cuts at: 0 703 1770 Size: 703 1067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1768 1770 Size: 1768 2 AlwNI CAG_nnn'CTG Cuts at: 0 411 435 1159 1674 1770 Size: 411 24 724 515 96 Fragments arranged by size: 724 515 411 96 24 ApaI G_GGCC'C Cuts at: 0 834 989 1687 1739 1770 Size: 834 155 698 52 31 Fragments arranged by size: 834 698 155 52 31 ApaLI G'TGCA_C Cuts at: 0 1608 1770 Size: 1608 162 ApoI r'AATT_y Cuts at: 0 472 1770 Size: 472 1298 AvaI C'yCGr_G Cuts at: 0 88 679 856 1177 1293 1404 1474 1546 Size: 88 591 177 321 116 111 70 72 Cuts at: 1546 1731 1770 Size: 185 39 Fragments arranged by size: 591 321 185 177 116 111 88 72 70 39 BamHI G'GATC_C Cuts at: 0 1046 1443 1515 1770 Size: 1046 397 72 255 Fragments arranged by size: 1046 397 255 72 BanI G'GyrC_C Cuts at: 0 61 105 226 312 705 821 1539 1618 Size: 61 44 121 86 393 116 718 79 Cuts at: 1618 1744 1770 Size: 126 26 Fragments arranged by size: 718 393 126 121 116 86 79 61 44 26 BanII G_rGCy'C Cuts at: 0 834 989 1177 1307 1400 1687 1739 1770 Size: 834 155 188 130 93 287 52 31 Fragments arranged by size: 834 287 188 155 130 93 52 31 BbeI G_GCGC'C Cuts at: 0 65 109 230 316 825 1543 1770 Size: 65 44 121 86 509 718 227 Fragments arranged by size: 718 509 227 121 86 65 44 BbsI GAAGACnn'nnnn_ Cuts at: 0 390 537 909 1770 Size: 390 147 372 861 Fragments arranged by size: 861 390 372 147 BbvCI CC'TCA_GC Cuts at: 0 268 1770 Size: 268 1502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 528 562 1770 Size: 528 34 1208 Fragments arranged by size: 1208 528 34 BglI GCCn_nnn'nGGC Cuts at: 0 324 371 883 1156 1270 1770 Size: 324 47 512 273 114 500 Fragments arranged by size: 512 500 324 273 114 47 BlpI GC'TnA_GC Cuts at: 0 887 1213 1770 Size: 887 326 557 Fragments arranged by size: 887 557 326 Bme1580I G_kGCm'C Cuts at: 0 710 834 863 989 1612 1687 1739 1747 Size: 710 124 29 126 623 75 52 8 Cuts at: 1747 1770 Size: 23 Fragments arranged by size: 710 623 126 124 75 52 29 23 8 BmgBI CAC'GTC Cuts at: 0 783 1770 Size: 783 987 BmrI ACTGGGnnnn_n' Cuts at: 0 601 827 1770 Size: 601 226 943 Fragments arranged by size: 943 601 226 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 513 1464 1500 1770 Size: 513 951 36 270 Fragments arranged by size: 951 513 270 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 219 604 1434 1770 Size: 208 11 385 830 336 Fragments arranged by size: 830 385 336 208 11 Bpu10I CC'TnA_GC Cuts at: 0 268 1770 Size: 268 1502 BsaI GGTCTCn'nnnn_ Cuts at: 0 393 517 940 1770 Size: 393 124 423 830 Fragments arranged by size: 830 423 393 124 BsaAI yAC'GTr Cuts at: 0 813 1062 1209 1770 Size: 813 249 147 561 Fragments arranged by size: 813 561 249 147 BsaHI Gr'CG_yC Cuts at: 0 62 106 227 313 604 610 822 1037 Size: 62 44 121 86 291 6 212 215 Cuts at: 1037 1540 1770 Size: 503 230 Fragments arranged by size: 503 291 230 215 212 121 86 62 44 6 BsaWI w'CCGG_w Cuts at: 0 112 1518 1770 Size: 112 1406 252 Fragments arranged by size: 1406 252 112 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 630 639 642 705 1294 1770 Size: 630 9 3 63 589 476 Fragments arranged by size: 630 589 476 63 9 3 BseYI C'CCAG_C Cuts at: 0 306 1159 1347 1644 1674 1770 Size: 306 853 188 297 30 96 Fragments arranged by size: 853 306 297 188 96 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 763 1555 1770 Size: 763 792 215 Fragments arranged by size: 792 763 215 BsiHKAI G_wGCw'C Cuts at: 0 1187 1214 1612 1770 Size: 1187 27 398 158 Fragments arranged by size: 1187 398 158 27 BsmBI CGTCTCn'nnnn_ Cuts at: 0 642 928 1770 Size: 642 286 842 Fragments arranged by size: 842 642 286 Bsp1286I G_dGCh'C Cuts at: 0 710 834 863 989 1177 1187 1214 1307 Size: 710 124 29 126 188 10 27 93 Cuts at: 1307 1400 1612 1687 1739 1747 1770 Size: 93 212 75 52 8 23 Fragments arranged by size: 710 212 188 126 124 93 93 75 52 29 27 23 10 8 BspEI T'CCGG_A Cuts at: 0 112 1770 Size: 112 1658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1673 1770 Size: 1673 97 BsrBI CCG'CTC Cuts at: 0 1166 1770 Size: 1166 604 BsrFI r'CCGG_y Cuts at: 0 293 1590 1770 Size: 293 1297 180 Fragments arranged by size: 1297 293 180 BssHII G'CGCG_C Cuts at: 0 160 1770 Size: 160 1610 BssSI C'ACGA_G Cuts at: 0 1041 1770 Size: 1041 729 BstAPI GCAn_nnn'nTGC Cuts at: 0 867 1191 1770 Size: 867 324 579 Fragments arranged by size: 867 579 324 BstXI CCAn_nnnn'nTGG Cuts at: 0 243 507 817 1770 Size: 243 264 310 953 Fragments arranged by size: 953 310 264 243 BstYI r'GATC_y Cuts at: 0 1046 1443 1515 1770 Size: 1046 397 72 255 Fragments arranged by size: 1046 397 255 72 Bsu36I CC'TnA_GG Cuts at: 0 792 1770 Size: 792 978 BtgI C'CryG_G Cuts at: 0 57 335 369 900 1026 1528 1637 1739 Size: 57 278 34 531 126 502 109 102 Cuts at: 1739 1756 1770 Size: 17 14 Fragments arranged by size: 531 502 278 126 109 102 57 34 17 14 BtsI GCAGTG_nn' Cuts at: 0 27 1770 Size: 27 1743 DraIII CAC_nnn'GTG Cuts at: 0 1191 1770 Size: 1191 579 EaeI y'GGCC_r Cuts at: 0 203 1148 1230 1460 1525 1770 Size: 203 945 82 230 65 245 Fragments arranged by size: 945 245 230 203 82 65 EarI CTCTTCn'nnn_ Cuts at: 0 648 660 666 1165 1770 Size: 648 12 6 499 605 Fragments arranged by size: 648 605 499 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 44 274 1039 1770 Size: 44 230 765 731 Fragments arranged by size: 765 731 230 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 219 604 1434 1528 1770 Size: 208 11 385 830 94 242 Fragments arranged by size: 830 385 242 208 94 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 700 724 1510 1770 Size: 700 24 786 260 Fragments arranged by size: 786 700 260 24 EcoO109I rG'GnC_Cy Cuts at: 0 985 986 1362 1503 1533 1551 1602 1684 Size: 985 1 376 141 30 18 51 82 Cuts at: 1684 1735 1770 Size: 51 35 Fragments arranged by size: 985 376 141 82 51 51 35 30 18 1 HaeII r_GCGC'y Cuts at: 0 65 109 230 316 385 735 825 1543 Size: 65 44 121 86 69 350 90 718 Cuts at: 1543 1770 Size: 227 Fragments arranged by size: 718 350 227 121 90 86 69 65 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 407 439 896 928 1270 1302 1662 1694 Size: 407 32 457 32 342 32 360 32 Cuts at: 1694 1770 Size: 76 Fragments arranged by size: 457 407 360 342 76 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 208 1770 Size: 208 1562 KasI G'GCGC_C Cuts at: 0 61 105 226 312 821 1539 1770 Size: 61 44 121 86 509 718 231 Fragments arranged by size: 718 509 231 121 86 61 44 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1348 1770 Size: 1348 422 MscI TGG'CCA Cuts at: 0 205 1150 1232 1770 Size: 205 945 82 538 Fragments arranged by size: 945 538 205 82 MslI CAynn'nnrTG Cuts at: 0 703 811 1390 1770 Size: 703 108 579 380 Fragments arranged by size: 703 579 380 108 MspA1I CmG'CkG Cuts at: 0 59 219 243 337 349 371 1010 1351 Size: 59 160 24 94 12 22 639 341 Cuts at: 1351 1639 1741 1758 1770 Size: 288 102 17 12 Fragments arranged by size: 639 341 288 160 102 94 59 24 22 17 12 12 NarI GG'CG_CC Cuts at: 0 62 106 227 313 822 1540 1770 Size: 62 44 121 86 509 718 230 Fragments arranged by size: 718 509 230 121 86 62 44 NcoI C'CATG_G Cuts at: 0 900 1528 1770 Size: 900 628 242 Fragments arranged by size: 900 628 242 PasI CC'CwG_GG Cuts at: 0 74 320 562 840 1374 1716 1770 Size: 74 246 242 278 534 342 54 Fragments arranged by size: 534 342 278 246 242 74 54 PflMI CCAn_nnn'nTGG Cuts at: 0 840 901 1770 Size: 840 61 869 Fragments arranged by size: 869 840 61 PfoI T'CCnGG_A Cuts at: 0 148 725 1694 1770 Size: 148 577 969 76 Fragments arranged by size: 969 577 148 76 PmlI CAC'GTG Cuts at: 0 813 1062 1209 1770 Size: 813 249 147 561 Fragments arranged by size: 813 561 249 147 PpuMI rG'GwC_Cy Cuts at: 0 1503 1551 1770 Size: 1503 48 219 Fragments arranged by size: 1503 219 48 PshAI GACnn'nnGTC Cuts at: 0 1079 1770 Size: 1079 691 PspOMI G'GGCC_C Cuts at: 0 830 985 1683 1735 1770 Size: 830 155 698 52 35 Fragments arranged by size: 830 698 155 52 35 PstI C_TGCA'G Cuts at: 0 128 1115 1142 1670 1770 Size: 128 987 27 528 100 Fragments arranged by size: 987 528 128 100 27 PvuII CAG'CTG Cuts at: 0 243 349 1010 1770 Size: 243 106 661 760 Fragments arranged by size: 760 661 243 106 RsrII CG'GwC_CG Cuts at: 0 115 1770 Size: 115 1655 SacII CC_GC'GG Cuts at: 0 60 338 372 1640 1742 1759 1770 Size: 60 278 34 1268 102 17 11 Fragments arranged by size: 1268 278 102 60 34 17 11 SanDI GG'GwC_CC Cuts at: 0 1551 1770 Size: 1551 219 SapI GCTCTTCn'nnn_ Cuts at: 0 1165 1770 Size: 1165 605 SbfI CC_TGCA'GG Cuts at: 0 128 1770 Size: 128 1642 SfcI C'TryA_G Cuts at: 0 124 1111 1138 1666 1770 Size: 124 987 27 528 104 Fragments arranged by size: 987 528 124 104 27 SfiI GGCCn_nnn'nGGCC Cuts at: 0 371 1770 Size: 371 1399 SfoI GGC'GCC Cuts at: 0 63 107 228 314 823 1541 1770 Size: 63 44 121 86 509 718 229 Fragments arranged by size: 718 509 229 121 86 63 44 SgrAI Cr'CCGG_yG Cuts at: 0 1590 1770 Size: 1590 180 SmaI CCC'GGG Cuts at: 0 90 1179 1295 1548 1733 1770 Size: 90 1089 116 253 185 37 Fragments arranged by size: 1089 253 185 116 90 37 StuI AGG'CCT Cuts at: 0 790 1702 1770 Size: 790 912 68 Fragments arranged by size: 912 790 68 StyI C'CwwG_G Cuts at: 0 701 900 1528 1770 Size: 701 199 628 242 Fragments arranged by size: 701 628 242 199 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1225 1770 Size: 1225 545 XmaI C'CCGG_G Cuts at: 0 88 1177 1293 1546 1731 1770 Size: 88 1089 116 253 185 39 Fragments arranged by size: 1089 253 185 116 88 39 Enzymes that do cut: AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NarI NcoI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII RsrII SacII SanDI SapI SbfI SfcI SfiI SfoI SgrAI SmaI StuI StyI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmtI BpuEI BsaBI BsaXI BsiEI BsiWI BsmI BspHI BsrDI BsrGI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI SacI SalI ScaI SexAI SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI