(Circular) (Six-base) MAPSORT of: pFN21AE3316.seq Check: 800 from: 1 to: 7339 pFN21AE3316 7339 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4360 4360 Size: 7339 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5149 278 Size: 53 83 186 936 3613 2468 Fragments arranged by size: 3613 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2741 3648 4682 1937 Size: 804 907 1034 4594 Fragments arranged by size: 4594 1034 907 804 Acc65I G'GTAC_C Cuts at: 4660 4660 Size: 7339 AclI AA'CG_TT Cuts at: 1563 5466 5839 1563 Size: 3903 373 3063 Fragments arranged by size: 3903 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2533 2682 3352 3527 5402 6786 7054 1632 Size: 901 149 670 175 1875 1384 268 1917 Fragments arranged by size: 1917 1875 1384 901 670 268 175 149 AfeI AGC'GCT Cuts at: 2540 2540 Size: 7339 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7142 AflIII A'CryG_T Cuts at: 1203 3903 4041 4203 6238 7215 1203 Size: 2700 138 162 2035 977 1327 Fragments arranged by size: 2700 2035 1327 977 162 138 AgeI A'CCGG_T Cuts at: 6170 6170 Size: 7339 AhdI GACnn_n'nnGTC Cuts at: 2305 4207 6068 2305 Size: 1902 1861 3576 Fragments arranged by size: 3576 1902 1861 AleI CACnn'nnGTG Cuts at: 1166 3087 1166 Size: 1921 5418 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3215 1184 Size: 2031 5308 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2228 2260 3836 3868 2228 Size: 32 1576 32 5699 Fragments arranged by size: 5699 1576 32 32 AlwNI CAG_nnn'CTG Cuts at: 2881 3526 3817 3979 4320 4615 5082 6654 2881 Size: 645 291 162 341 295 467 1572 3566 Fragments arranged by size: 3566 1572 645 467 341 295 291 162 ApaI G_GGCC'C Cuts at: 2578 2578 Size: 7339 ApaLI G'TGCA_C Cuts at: 2432 5396 6552 2432 Size: 2964 1156 3219 Fragments arranged by size: 3219 2964 1156 ApoI r'AATT_y Cuts at: 1493 3324 4648 4874 5026 7135 1493 Size: 1831 1324 226 152 2109 1697 Fragments arranged by size: 2109 1831 1697 1324 226 152 AseI AT'TA_AT Cuts at: 160 1032 5893 160 Size: 872 4861 1606 Fragments arranged by size: 4861 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7339 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2125 3495 3827 4163 4664 1870 Size: 73 105 77 1370 332 336 501 4545 Fragments arranged by size: 4545 1370 501 336 332 105 77 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4415 4448 4415 Size: 33 7306 BamHI G'GATC_C Cuts at: 4669 5036 4669 Size: 367 6972 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2446 2586 2633 2791 3261 Size: 325 217 627 659 140 47 158 470 Cuts at: 3261 3386 4159 4244 4660 6115 618 Size: 125 773 85 416 1455 1842 Fragments arranged by size: 1842 1455 773 659 627 470 416 325 217 158 140 125 85 47 BanII G_rGCy'C Cuts at: 1391 2037 2578 3128 3680 4658 1391 Size: 646 541 550 552 978 4072 Fragments arranged by size: 4072 978 646 552 550 541 BbeI G_GCGC'C Cuts at: 2450 2590 3265 2450 Size: 140 675 6524 Fragments arranged by size: 6524 675 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3725 928 Size: 971 1826 4542 Fragments arranged by size: 4542 1826 971 BbvCI CC'TCA_GC Cuts at: 3486 4238 3486 Size: 752 6587 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2423 2457 5530 5564 1903 Size: 34 486 34 3073 34 3678 Fragments arranged by size: 3678 3073 486 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5231 6441 5231 Size: 1210 6129 BclI T'GATC_A Cuts at: 1548 2461 4221 1548 Size: 913 1760 4666 Fragments arranged by size: 4666 1760 913 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3475 4157 4242 Size: 107 122 71 631 605 1803 682 85 Cuts at: 4242 5950 136 Size: 1708 1525 Fragments arranged by size: 1803 1708 1525 682 631 605 122 107 85 71 BglII A'GATC_T Cuts at: 3654 7334 3654 Size: 3680 3659 BlpI GC'TnA_GC Cuts at: 2669 4756 2669 Size: 2087 5252 Bme1580I G_kGCm'C Cuts at: 1790 2436 2578 3389 4145 5400 6556 1790 Size: 646 142 811 756 1255 1156 2573 Fragments arranged by size: 2573 1255 1156 811 756 646 142 BmgBI CAC'GTC Cuts at: 1318 2307 2601 3733 4440 1318 Size: 989 294 1132 707 4217 Fragments arranged by size: 4217 1132 989 707 294 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5070 6028 442 Size: 416 53 83 399 24 3653 958 1753 Fragments arranged by size: 3653 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7339 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3863 4342 4359 4481 5999 1239 Size: 2624 479 17 122 1518 2579 Fragments arranged by size: 2624 2579 1518 479 122 17 Bpu10I CC'TnA_GC Cuts at: 3486 3679 3945 4238 3486 Size: 193 266 293 6587 Fragments arranged by size: 6587 293 266 193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2489 2843 5464 6329 6627 2489 Size: 354 2621 865 298 3201 Fragments arranged by size: 3201 2621 865 354 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2064 2143 6002 882 Size: 617 565 79 3859 2219 Fragments arranged by size: 3859 2219 617 565 79 BsaAI yAC'GTr Cuts at: 493 1204 5130 493 Size: 711 3926 2702 Fragments arranged by size: 3926 2702 711 BsaBI GATnn'nnATC Cuts at: 7333 7333 Size: 7339 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2447 2587 3262 3860 Size: 53 83 186 936 914 140 675 598 Cuts at: 3860 5146 5528 6163 275 Size: 1286 382 635 1451 Fragments arranged by size: 1451 1286 936 914 675 635 598 382 186 140 83 53 BsaWI w'CCGG_w Cuts at: 2339 3044 3714 3789 5772 6170 6444 6591 7002 Size: 705 670 75 1983 398 274 147 411 Cuts at: 7002 2339 Size: 2676 Fragments arranged by size: 2676 1983 705 670 411 398 274 147 75 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3836 3866 1178 Size: 30 165 30 2433 30 4651 Fragments arranged by size: 4651 2433 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2052 2713 2846 2999 3107 3110 1186 Size: 866 661 133 153 108 3 5415 Fragments arranged by size: 5415 866 661 153 133 108 3 BseYI C'CCAG_C Cuts at: 3501 3554 3673 4512 6542 7038 3501 Size: 53 119 839 2030 496 3802 Fragments arranged by size: 3802 2030 839 496 119 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2638 1163 Size: 573 902 5864 Fragments arranged by size: 5864 902 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4789 5550 6578 1540 Size: 275 186 2788 761 1028 2301 Fragments arranged by size: 2788 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2436 3128 5400 5485 6556 2436 Size: 692 2272 85 1071 3219 Fragments arranged by size: 3219 2272 1071 692 85 BsmI GAATG_Cn' Cuts at: 3079 4850 4943 3079 Size: 1771 93 5475 Fragments arranged by size: 5475 1771 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2607 3851 2607 Size: 1244 6095 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2436 2578 3128 3389 3680 4145 Size: 399 247 399 142 550 261 291 465 Cuts at: 4145 4658 5400 5485 6556 1391 Size: 513 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 550 513 465 399 399 291 261 247 142 85 BspEI T'CCGG_A Cuts at: 2339 3789 7002 2339 Size: 1450 3213 2676 Fragments arranged by size: 3213 2676 1450 BspHI T'CATG_A Cuts at: 5228 6958 5228 Size: 1730 5609 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4360 4371 4694 844 Size: 3516 11 323 3489 Fragments arranged by size: 3516 3489 323 11 BsrBI CCG'CTC Cuts at: 1394 1823 4131 4194 5226 1394 Size: 429 2308 63 1032 3507 Fragments arranged by size: 3507 2308 1032 429 63 BsrDI GCAATG_nn' Cuts at: 65 1248 2891 3636 5834 6008 65 Size: 1183 1643 745 2198 174 1396 Fragments arranged by size: 2198 1643 1396 1183 745 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3936 4147 5983 6170 1700 Size: 110 2126 211 1836 187 2869 Fragments arranged by size: 2869 2126 1836 211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7339 BssHII G'CGCG_C Cuts at: 1925 4508 1925 Size: 2583 4756 BssSI C'ACGA_G Cuts at: 5399 6411 5399 Size: 1012 6327 BstAPI GCAn_nnn'nTGC Cuts at: 794 2329 2881 2895 3166 3817 4540 794 Size: 1535 552 14 271 651 723 3593 Fragments arranged by size: 3593 1535 723 651 552 271 14 BstBI TT'CG_AA Cuts at: 6143 6143 Size: 7339 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7339 BstXI CCAn_nnnn'nTGG Cuts at: 2605 2761 2605 Size: 156 7183 BstYI r'GATC_y Cuts at: 1972 2115 2280 2409 3618 3654 3975 4284 4570 Size: 143 165 129 1209 36 321 309 286 Cuts at: 4570 4669 5036 5421 5438 6890 6976 6988 7334 Size: 99 367 385 17 1452 86 12 346 Cuts at: 7334 1972 Size: 1977 Fragments arranged by size: 1977 1452 1209 385 367 346 321 309 286 165 143 129 99 86 36 17 12 BstZ17I GTA'TAC Cuts at: 2742 2742 Size: 7339 Bsu36I CC'TnA_GG Cuts at: 4071 4071 Size: 7339 BtgI C'CryG_G Cuts at: 513 1065 2004 4560 513 Size: 552 939 2556 3292 Fragments arranged by size: 3292 2556 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2526 487 Size: 683 56 1300 5300 Fragments arranged by size: 5300 1300 683 56 BtsI GCAGTG_nn' Cuts at: 2464 3397 3414 4855 5649 5669 2464 Size: 933 17 1441 794 20 4134 Fragments arranged by size: 4134 1441 933 794 20 17 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7339 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2551 2586 529 Size: 35 1987 35 5282 Fragments arranged by size: 5282 1987 35 35 DraI TTT'AAA Cuts at: 4643 4643 Size: 7339 DraIII CAC_nnn'GTG Cuts at: 2623 2623 Size: 7339 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3047 6346 809 Size: 507 1731 3299 1802 Fragments arranged by size: 3299 1802 1731 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2310 2388 Size: 54 1425 50 275 18 177 303 78 Cuts at: 2388 2607 2614 3801 4311 4786 5675 7304 8 Size: 219 7 1187 510 475 889 1629 43 Fragments arranged by size: 1629 1425 1187 889 510 475 303 275 219 177 78 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 4786 1537 Size: 275 2974 4090 Fragments arranged by size: 4090 2974 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2486 2658 2722 2731 2873 3293 5269 1353 Size: 1133 172 64 9 142 420 1976 3423 Fragments arranged by size: 3423 1976 1133 420 172 142 64 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2055 2402 3346 3506 4115 4634 Size: 1064 138 466 347 944 160 609 519 Cuts at: 4634 5926 6300 6446 387 Size: 1292 374 146 1280 Fragments arranged by size: 1292 1280 1064 944 609 519 466 374 347 160 146 138 EcoICRI GAG'CTC Cuts at: 3126 3126 Size: 7339 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2533 2682 3352 3527 3863 4342 4359 Size: 393 901 149 670 175 336 479 17 Cuts at: 4359 4481 5402 5999 6786 7054 1239 Size: 122 921 597 787 268 1524 Fragments arranged by size: 1524 921 901 787 670 597 479 393 336 268 175 149 122 17 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2951 4367 2366 Size: 585 1416 5338 Fragments arranged by size: 5338 1416 585 EcoO109I rG'GnC_Cy Cuts at: 2500 2574 2863 2878 2956 3523 4581 2500 Size: 74 289 15 78 567 1058 5258 Fragments arranged by size: 5258 1058 567 289 78 74 15 EcoRI G'AATT_C Cuts at: 4648 5026 4648 Size: 378 6961 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2884 2884 Size: 7339 FspI TGC'GCA Cuts at: 4382 5845 4382 Size: 1463 5876 HaeII r_GCGC'y Cuts at: 1678 2450 2542 2590 3021 3265 3561 3952 6486 Size: 772 92 48 431 244 296 391 2534 Cuts at: 6486 1678 Size: 2531 Fragments arranged by size: 2534 2531 772 431 391 296 244 92 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2291 2323 4193 Size: 32 135 32 503 32 184 32 1870 Cuts at: 4193 4225 5980 6012 6054 6086 1373 Size: 32 1755 32 42 32 2626 Fragments arranged by size: 2626 1870 1755 503 184 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4683 4929 669 Size: 988 281 2745 246 3079 Fragments arranged by size: 3079 2745 988 281 246 HindIII A'AGCT_T Cuts at: 748 3585 748 Size: 2837 4502 HpaI GTT'AAC Cuts at: 4929 4929 Size: 7339 KasI G'GCGC_C Cuts at: 2446 2586 3261 2446 Size: 140 675 6524 Fragments arranged by size: 6524 675 140 KpnI G_GTAC'C Cuts at: 4664 4664 Size: 7339 MfeI C'AATT_G Cuts at: 4938 4938 Size: 7339 MluI A'CGCG_T Cuts at: 7215 7215 Size: 7339 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3633 3888 6453 6637 1305 Size: 2328 255 2565 184 2007 Fragments arranged by size: 2565 2328 2007 255 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2609 2616 3803 10 Size: 54 1425 343 177 600 7 1187 3546 Fragments arranged by size: 3546 1425 1187 600 343 177 54 7 MslI CAynn'nnrTG Cuts at: 518 1166 3087 3602 4497 5297 5656 5815 518 Size: 648 1921 515 895 800 359 159 2042 Fragments arranged by size: 2042 1921 895 800 648 515 359 159 MspA1I CmG'CkG Cuts at: 1606 2218 2326 3331 4242 4337 4540 4755 5432 Size: 612 108 1005 911 95 203 215 677 Cuts at: 5432 6580 6825 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1005 911 677 612 245 215 203 108 95 NaeI GCC'GGC Cuts at: 3938 4149 3938 Size: 211 7128 NarI GG'CG_CC Cuts at: 2447 2587 3262 2447 Size: 140 675 6524 Fragments arranged by size: 6524 675 140 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5848 Fragments arranged by size: 5848 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7339 NgoMIV G'CCGG_C Cuts at: 3936 4147 3936 Size: 211 7128 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7339 NotI GC'GGCC_GC Cuts at: 4786 4786 Size: 7339 NspI r_CATG'y Cuts at: 1132 1229 3853 3907 4207 4609 4697 6242 1132 Size: 97 2624 54 300 402 88 1545 2229 Fragments arranged by size: 2624 2229 1545 402 300 97 88 54 PasI CC'CwG_GG Cuts at: 1348 3130 1348 Size: 1782 5557 PciI A'CATG_T Cuts at: 3903 4203 6238 3903 Size: 300 2035 5004 Fragments arranged by size: 5004 2035 300 PflMI CCAn_nnn'nTGG Cuts at: 1108 3979 4320 1108 Size: 2871 341 4127 Fragments arranged by size: 4127 2871 341 PfoI T'CCnGG_A Cuts at: 2349 2349 Size: 7339 PmeI GTTT'AAAC Cuts at: 4643 4643 Size: 7339 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3836 3868 4106 4138 5408 5440 6921 6953 3836 Size: 32 238 32 1270 32 1481 32 4222 Fragments arranged by size: 4222 1481 1270 238 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7339 PsiI TTA'TAA Cuts at: 4909 4909 Size: 7339 PspOMI G'GGCC_C Cuts at: 2574 2574 Size: 7339 PstI C_TGCA'G Cuts at: 830 2091 2325 3813 4371 4691 830 Size: 1261 234 1488 558 320 3478 Fragments arranged by size: 3478 1488 1261 558 320 234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7339 PvuII CAG'CTG Cuts at: 2218 2326 4337 2218 Size: 108 2011 5220 Fragments arranged by size: 5220 2011 108 SacI G_AGCT'C Cuts at: 3128 3128 Size: 7339 SalI G'TCGA_C Cuts at: 1936 4681 1936 Size: 2745 4594 SapI GCTCTTCn'nnn_ Cuts at: 2486 2658 3293 2486 Size: 172 635 6532 Fragments arranged by size: 6532 635 172 SbfI CC_TGCA'GG Cuts at: 4691 4691 Size: 7339 ScaI AGT'ACT Cuts at: 5587 7299 5587 Size: 1712 5627 SexAI A'CCwGG_T Cuts at: 2361 2361 Size: 7339 SfcI C'TryA_G Cuts at: 826 1045 2087 2321 2566 3809 4367 4390 4687 Size: 219 1042 234 245 1243 558 23 297 Cuts at: 4687 5822 6503 6694 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1243 1135 1042 681 558 297 245 234 219 191 23 SfoI GGC'GCC Cuts at: 2448 2588 3263 2448 Size: 140 675 6524 Fragments arranged by size: 6524 675 140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7339 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7339 SmaI CCC'GGG Cuts at: 1872 2050 2127 3497 3829 4666 1872 Size: 178 77 1370 332 837 4545 Fragments arranged by size: 4545 1370 837 332 178 77 SmlI C'TyrA_G Cuts at: 820 1017 1943 2504 2858 5443 6344 6606 820 Size: 197 926 561 354 2585 901 262 1553 Fragments arranged by size: 2585 1553 926 901 561 354 262 197 SnaBI TAC'GTA Cuts at: 493 5130 493 Size: 4637 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7339 SphI G_CATG'C Cuts at: 1132 4697 1132 Size: 3565 3774 SspI AAT'ATT Cuts at: 5 52 5263 5 Size: 47 5211 2081 Fragments arranged by size: 5211 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7339 StyI C'CwwG_G Cuts at: 513 1065 2004 2781 4778 513 Size: 552 939 777 1997 3074 Fragments arranged by size: 3074 1997 939 777 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3202 3568 3986 5366 5383 5536 5721 Size: 1255 1622 366 418 1380 17 153 185 Cuts at: 5721 6166 325 Size: 445 1498 Fragments arranged by size: 1622 1498 1380 1255 445 418 366 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5585 7297 96 Size: 274 80 33 51 1443 3608 1712 138 Fragments arranged by size: 3608 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3260 3292 3448 3480 7299 7331 3260 Size: 32 156 32 3819 32 3268 Fragments arranged by size: 3819 3268 156 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7339 XbaI T'CTAG_A Cuts at: 4675 4675 Size: 7339 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3433 4334 3433 Size: 901 6438 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7339 XmaI C'CCGG_G Cuts at: 1870 2048 2125 3495 3827 4664 1870 Size: 178 77 1370 332 837 4545 Fragments arranged by size: 4545 1370 837 332 178 77 XmnI GAAnn'nnTTC Cuts at: 4121 5468 4121 Size: 1347 5992 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5147 276 Size: 53 83 186 936 3613 2468 Fragments arranged by size: 3613 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BplI BsiWI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI