################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstPI BstUI BstXI BstYI CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AfaI 524 GTAC GT^AC AlwI 408 GGATC GGATC(4/5) AocI 480 CCTGAGG CC^T[ACGT]AGG AspHI 496 GTGCTC G[AT]GC[AT]^C AvaII 485 GGTCC G^G[AT]CC BalI 568 TGGCCA TGG^CCA BglI 430 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 533 CTCCAG CTGGAG(16/14) BsiHKAI 496 GTGCTC G[AT]GC[AT]^C BsiYI 219 CCAGCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 219 CCAGCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 67 GAGAC GTCTC(1/5) BsmFI 3 GTCCC GGGAC(10/14) BssSI 213 CTCGTG CACGAG(-5/-1) Bst1107I 313 GTATAC GTA^TAC BstAPI 191 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 313 GTATAC GTA^TAC Bsu36I 480 CCTGAGG CC^T[ACGT]AGG CfoI 137 GCGC GCG^C DpnII 409 GATC ^GATC DraII 119 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 324 CCGTGG C^C[GA][CT]GG EaeI 568 TGGCCA [CT]^GGCC[GA] Eco81I 480 CCTGAGG CC^T[ACGT]AGG EcoO109I 119 AGGCCCT [GA]G^G[ACGT]CC[CT] HhaI 137 GCGC GCG^C HinP1I 137 GCGC G^CGC HinfI 50 GACTC G^A[ACGT]TC MaeII 275 ACGT A^CGT MaeIII 356 GTCAC ^GT[ACGT]AC MboI 409 GATC ^GATC MluNI 568 TGGCCA TGG^CCA MscI 568 TGGCCA TGG^CCA MspA1I 195 CAGCTG C[AC]G^C[GT]G NdeII 409 GATC ^GATC PleI 50 GACTC GAGTC(4/5) PstI 87 CTGCAG CTGCA^G PvuII 195 CAGCTG CAG^CTG RsaI 524 GTAC GT^AC Sau3AI 409 GATC ^GATC SfcI 87 CTGCAG C^T[GA][CT]AG TaqI 48 TCGA T^CGA Tsp45I 356 GTCAC ^GT[GC]AC Tsp509I 565 AATT ^AATT TspRI 260 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 48 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AccI 313 GTATAC GT^[AC][GT]AC 449 GTCTAC AlwNI 142 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 192 CAGCAGCTG BanI 343 GGTGCC G^G[CT][GA]CC 414 GGTGCC BsgI 238 GTGCAG GTGCAG(16/14) 517 GTGCAG BsrI 261 ACTGG ACTGG(1/-1) 453 ACTGG MslI 128 CATCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 508 CACCGGGATG SfaNI 127 GCATC GCATC(5/9) 383 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI BanII BbsI BbvI BcnI BmyI BpuAI BsaJI Bsp1286I BsrBI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI