################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BseAI BseRI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI Ksp632I MamI MfeI MluI MroI MslI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tsp509I TspRI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AlwI 114 GATCC GGATC(4/5) AocI 337 CCTGAGG CC^T[ACGT]AGG ApaI 759 GGGCCC GGGCC^C BbeI 448 GGCGCC GGCGC^C BbsI 604 GAAGAC GAAGAC(2/6) BclI 431 TGATCA T^GATCA BfaI 250 CTAG C^TAG BglII 1322 AGATCT A^GATCT BpuAI 604 GAAGAC GAAGAC(2/6) BsaI 181 GAGACC GGTCTC(1/5) BsaWI 184 ACCGGA [AT]^CCGG[AT] BsiWI 689 CGTACG C^GTACG BsmAI 181 GAGAC GTCTC(1/5) BsmI 276 GAATGC GAATGC(1/-1) BstAPI 617 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1322 AGATCT [GA]^GATC[CT] Bsu36I 337 CCTGAGG CC^T[ACGT]AGG Eco81I 337 CCTGAGG CC^T[ACGT]AGG EcoT14I 1214 CCAAGG C^C[AT][AT]GG FbaI 431 TGATCA T^GATCA HincII 346 GTCAAC GT[CT]^[GA]AC HindII 346 GTCAAC GT[CT]^[GA]AC HinfI 19 GATTC G^A[ACGT]TC KasI 448 GGCGCC G^GCGCC MaeI 250 CTAG C^TAG MboII 604 GAAGA GAAGA(8/7) MflI 1322 AGATCT [GA]^GATC[CT] MseI 848 TTAA T^TAA NarI 448 GGCGCC GG^CGCC NlaIII 485 CATG CATG^ NotI 721 GCGGCCGC GC^GGCCGC PflMI 1464 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1060 GGGTCCT [GA]G^G[AT]CC[CT] SbfI 522 CCTGCAGG CCTGCA^GG SexAI 1247 ACCTGGT A^CC[AT]GGT SfoI 448 GGCGCC GGC^GCC Sse8387I 522 CCTGCAGG CCTGCA^GG StuI 269 AGGCCT AGG^CCT StyI 1214 CCAAGG C^C[AT][AT]GG TaqI 38 TCGA T^CGA TfiI 19 GATTC G^A[AT]TC Tru9I 848 TTAA T^TAA TthHB8I 38 TCGA T^CGA Van91I 1464 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1322 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 448 GGCGCC G[GA]^CG[CT]C 919 GGCGTC Alw44I 283 GTGCAC G^TGCAC 565 GTGCAC AlwNI 618 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1297 CAGGAGCTG ApaLI 283 GTGCAC G^TGCAC 565 GTGCAC AspHI 283 GTGCAC G[AT]GC[AT]^C 565 GTGCAC AvaII 306 GGTCC G^G[AT]CC 1061 GGTCC BalI 662 TGGCCA TGG^CCA 830 TGGCCA BsaHI 448 GGCGCC G[GA]^CG[CT]C 919 GGCGTC BsiHKAI 283 GTGCAC G[AT]GC[AT]^C 565 GTGCAC BsmFI 409 GTCCC GGGAC(10/14) 498 GTCCC BsrBI 1403 CCGCTC CCGCTC(-3/-3) 238 GAGCGG BsrFI 679 ACCGGC [GA]^CCGG[CT] 1361 ACCGGC BstEII 1368 GGTCACC G^GT[ACGT]ACC 1428 GGTCACC BstPI 1368 GGTCACC G^GT[ACGT]ACC 1428 GGTCACC Cfr10I 679 ACCGGC [GA]^CCGG[CT] 1361 ACCGGC EcoNI 249 CCTAGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1263 CCTGGGGAAGG EcoO65I 1368 GGTCACC G^GT[ACGT]ACC 1428 GGTCACC HgaI 730 GCGTC GACGC(5/10) 920 GCGTC Hin1I 448 GGCGCC G[GA]^CG[CT]C 919 GGCGTC MaeII 647 ACGT A^CGT 1282 ACGT MluNI 662 TGGCCA TGG^CCA 830 TGGCCA MscI 662 TGGCCA TGG^CCA 830 TGGCCA XcmI 332 CCAAGCCTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1214 CCAAGGAGCTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AfaI AluI AvaI BanI BanII BbvI BcnI BglI BmyI BsaJI BsgI BsiEI BsiYI BslI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI KspI MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfiI TseI Tsp45I