(Circular) (Six-base) MAPSORT of: pFN21AE2647.seq Check: 2550 from: 1 to: 6181 pFN21AE2647 6181 bp With 182 enzymes: SgfI * November 11, 2016 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2557 2557 Size: 6181 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3524 1937 Size: 1587 4594 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 4244 5628 5896 1632 Size: 497 2115 1384 268 1917 Fragments arranged by size: 2115 1917 1384 497 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 5080 6057 1203 Size: 3877 977 1327 Fragments arranged by size: 3877 1327 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 4910 4910 Size: 6181 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2543 2846 1184 Size: 1359 303 4519 Fragments arranged by size: 4519 1359 303 AlwNI CAG_nnn'CTG Cuts at: 2628 3307 3924 5496 2628 Size: 679 617 1572 3313 Fragments arranged by size: 3313 1572 679 617 ApaI G_GGCC'C Cuts at: 2768 2768 Size: 6181 ApaLI G'TGCA_C Cuts at: 2288 2570 4238 5394 2288 Size: 282 1668 1156 3075 Fragments arranged by size: 3075 1668 1156 282 ApoI r'AATT_y Cuts at: 1493 3490 3716 3868 5977 1493 Size: 1997 226 152 2109 1697 Fragments arranged by size: 2109 1997 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4735 160 Size: 872 3703 1606 Fragments arranged by size: 3703 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2054 2561 3101 3506 1870 Size: 73 69 42 507 540 405 4545 Fragments arranged by size: 4545 540 507 405 73 69 42 BamHI G'GATC_C Cuts at: 3511 3878 3511 Size: 367 5814 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2453 2822 3241 3502 4957 Size: 325 217 627 666 369 419 261 1455 Cuts at: 4957 618 Size: 1842 Fragments arranged by size: 1842 1455 666 627 419 369 325 261 217 BanII G_rGCy'C Cuts at: 1391 2013 2141 2562 2768 3061 3500 1391 Size: 622 128 421 206 293 439 4072 Fragments arranged by size: 4072 622 439 421 293 206 128 BbeI G_GCGC'C Cuts at: 2457 2457 Size: 6181 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2616 928 Size: 971 717 4493 Fragments arranged by size: 4493 971 717 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4372 4406 1903 Size: 34 2435 34 3678 Fragments arranged by size: 3678 2435 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4073 5283 4073 Size: 1210 4971 BclI T'GATC_A Cuts at: 1548 2436 1548 Size: 888 5293 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2399 2654 2763 Size: 107 122 71 631 605 727 255 109 Cuts at: 2763 2777 2939 2948 3026 3173 4792 136 Size: 14 162 9 78 147 1619 1525 Fragments arranged by size: 1619 1525 727 631 605 255 162 147 122 109 107 78 71 14 9 BglII A'GATC_T Cuts at: 3327 6176 3327 Size: 2849 3332 BlpI GC'TnA_GC Cuts at: 3598 3598 Size: 6181 Bme1580I G_kGCm'C Cuts at: 1790 2267 2292 2574 2768 4242 5398 1790 Size: 477 25 282 194 1474 1156 2573 Fragments arranged by size: 2573 1474 1156 477 282 194 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3912 4870 442 Size: 416 53 83 399 24 2495 958 1753 Fragments arranged by size: 2495 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4841 1239 Size: 3602 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4306 5171 5469 4306 Size: 865 298 5018 Fragments arranged by size: 5018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2180 4844 882 Size: 617 681 2664 2219 Fragments arranged by size: 2664 2219 681 617 BsaAI yAC'GTr Cuts at: 493 1204 3972 493 Size: 711 2768 2702 Fragments arranged by size: 2768 2702 711 BsaBI GATnn'nnATC Cuts at: 6175 6175 Size: 6181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2454 2925 3988 4370 Size: 53 83 186 936 921 471 1063 382 Cuts at: 4370 5005 275 Size: 635 1451 Fragments arranged by size: 1451 1063 936 921 635 471 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2189 4614 5012 5286 5433 5844 2189 Size: 2425 398 274 147 411 2526 Fragments arranged by size: 2526 2425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2478 2508 2674 2704 2870 Size: 30 165 30 1075 30 166 30 166 Cuts at: 2870 2900 1178 Size: 30 4459 Fragments arranged by size: 4459 1075 166 166 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6181 BseYI C'CCAG_C Cuts at: 2123 2761 2956 5384 5880 2123 Size: 638 195 2428 496 2424 Fragments arranged by size: 2428 2424 638 496 195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2050 2192 2516 2615 1163 Size: 573 314 142 324 99 4729 Fragments arranged by size: 4729 573 324 314 142 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2730 2742 2913 3168 3177 3631 Size: 275 186 729 12 171 255 9 454 Cuts at: 3631 4392 5420 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 729 454 275 255 186 171 12 9 BsiHKAI G_wGCw'C Cuts at: 2292 2574 4242 4327 5398 2292 Size: 282 1668 85 1071 3075 Fragments arranged by size: 3075 1668 1071 282 85 BsiWI C'GTAC_G Cuts at: 2694 2694 Size: 6181 BsmI GAATG_Cn' Cuts at: 2287 3692 3785 2287 Size: 1405 93 4683 Fragments arranged by size: 4683 1405 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2141 2267 2292 2562 2574 2768 Size: 399 223 128 126 25 270 12 194 Cuts at: 2768 3061 3500 4242 4327 5398 1391 Size: 293 439 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 293 270 223 194 128 126 85 25 12 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 4070 5800 4070 Size: 1730 4451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2535 2557 2838 3536 844 Size: 1691 22 281 698 3489 Fragments arranged by size: 3489 1691 698 281 22 BsrBI CCG'CTC Cuts at: 1394 1823 2245 3410 4068 1394 Size: 429 422 1165 658 3507 Fragments arranged by size: 3507 1165 658 429 422 BsrDI GCAATG_nn' Cuts at: 65 1248 4676 4850 65 Size: 1183 3428 174 1396 Fragments arranged by size: 3428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2684 3366 4825 5012 1700 Size: 110 874 682 1459 187 2869 Fragments arranged by size: 2869 1459 874 682 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 4241 5253 4241 Size: 1012 5169 BstAPI GCAn_nnn'nTGC Cuts at: 794 2628 794 Size: 1834 4347 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 3373 3433 1184 Size: 2189 60 3932 Fragments arranged by size: 3932 2189 60 BstXI CCAn_nnnn'nTGG Cuts at: 2890 2983 3226 2890 Size: 93 243 5845 Fragments arranged by size: 5845 243 93 BstYI r'GATC_y Cuts at: 1972 3327 3511 3878 4263 4280 5732 5818 5830 Size: 1355 184 367 385 17 1452 86 12 Cuts at: 5830 6176 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1355 385 367 346 184 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2343 2343 Size: 6181 BtgI C'CryG_G Cuts at: 513 1065 2004 2457 2664 2706 2724 2937 3162 Size: 552 939 453 207 42 18 213 225 Cuts at: 3162 3393 513 Size: 231 3301 Fragments arranged by size: 3301 939 552 453 231 225 213 207 42 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2345 487 Size: 683 56 1119 4323 Fragments arranged by size: 4323 1119 683 56 BtsI GCAGTG_nn' Cuts at: 3697 4491 4511 3697 Size: 794 20 5367 Fragments arranged by size: 5367 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5188 809 Size: 507 3872 1802 Fragments arranged by size: 3872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2667 2727 2739 Size: 54 1425 50 275 18 837 60 12 Cuts at: 2739 2835 2910 2931 2940 3078 3150 3165 3174 Size: 96 75 21 9 138 72 15 9 Cuts at: 3174 3396 3628 4517 6146 8 Size: 222 232 889 1629 43 Fragments arranged by size: 1629 1425 889 837 275 232 222 138 96 75 72 60 54 50 43 21 18 15 12 9 9 EagI C'GGCC_G Cuts at: 1537 1812 2727 2739 2910 3165 3174 3628 1537 Size: 275 915 12 171 255 9 454 4090 Fragments arranged by size: 4090 915 454 275 255 171 12 9 EarI CTCTTCn'nnn_ Cuts at: 1353 4111 1353 Size: 2758 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3144 3209 4768 5142 5288 387 Size: 1064 138 1555 65 1559 374 146 1280 Fragments arranged by size: 1559 1555 1280 1064 374 146 138 65 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 4244 4841 5628 5896 1239 Size: 393 497 2115 597 787 268 1524 Fragments arranged by size: 2115 1524 787 597 497 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2258 3272 2258 Size: 1014 5167 EcoO109I rG'GnC_Cy Cuts at: 2017 2037 2660 2765 2790 3066 3456 2017 Size: 20 623 105 25 276 390 4742 Fragments arranged by size: 4742 623 390 276 105 25 20 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 FspI TGC'GCA Cuts at: 4687 4687 Size: 6181 HaeII r_GCGC'y Cuts at: 1678 2457 2589 2852 3318 5328 1678 Size: 779 132 263 466 2010 2531 Fragments arranged by size: 2531 2010 779 466 263 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2184 2216 3301 3333 4822 Size: 32 135 32 612 32 1085 32 1489 Cuts at: 4822 4854 4896 4928 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1489 1085 612 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2353 3525 3771 669 Size: 988 281 415 1172 246 3079 Fragments arranged by size: 3079 1172 988 415 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6181 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KasI G'GCGC_C Cuts at: 2453 2453 Size: 6181 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2700 5295 5479 1305 Size: 1395 2595 184 2007 Fragments arranged by size: 2595 2007 1395 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 2837 10 Size: 54 1425 343 837 168 3354 Fragments arranged by size: 3354 1425 837 343 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 4139 4498 4657 518 Size: 648 2973 359 159 2042 Fragments arranged by size: 2973 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2176 2500 2553 2628 2708 2726 3145 3155 Size: 570 324 53 75 80 18 419 10 Cuts at: 3155 3164 3395 3430 3597 4274 5422 5667 1606 Size: 9 231 35 167 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 570 419 324 245 231 167 80 75 53 35 18 10 9 NarI GG'CG_CC Cuts at: 2454 2454 Size: 6181 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4690 Fragments arranged by size: 4690 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 2727 3628 2727 Size: 901 5280 NspI r_CATG'y Cuts at: 1132 1229 3539 5084 1132 Size: 97 2310 1545 2229 Fragments arranged by size: 2310 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2890 3061 1348 Size: 1542 171 4468 Fragments arranged by size: 4468 1542 171 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 3475 1108 Size: 2367 3814 PfoI T'CCnGG_A Cuts at: 3052 3052 Size: 6181 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4250 4282 5763 5795 4250 Size: 32 1481 32 4636 Fragments arranged by size: 4636 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3066 3066 Size: 6181 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PspOMI G'GGCC_C Cuts at: 2764 2764 Size: 6181 PstI C_TGCA'G Cuts at: 830 2181 2532 3160 3533 830 Size: 1351 351 628 373 3478 Fragments arranged by size: 3478 1351 628 373 351 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2176 2500 2628 2176 Size: 324 128 5729 Fragments arranged by size: 5729 324 128 SacII CC_GC'GG Cuts at: 2709 2727 3165 3396 2709 Size: 18 438 231 5494 Fragments arranged by size: 5494 438 231 18 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SbfI CC_TGCA'GG Cuts at: 2532 3533 2532 Size: 1001 5180 ScaI AGT'ACT Cuts at: 4429 6141 4429 Size: 1712 4469 SexAI A'CCwGG_T Cuts at: 3252 3252 Size: 6181 SfcI C'TryA_G Cuts at: 826 1045 2177 2528 3156 3529 4664 5345 5536 Size: 219 1132 351 628 373 1135 681 191 Cuts at: 5536 826 Size: 1471 Fragments arranged by size: 1471 1135 1132 681 628 373 351 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2763 2939 2948 3173 2763 Size: 176 9 225 5771 Fragments arranged by size: 5771 225 176 9 SfoI GGC'GCC Cuts at: 2455 2455 Size: 6181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 3508 1872 Size: 1636 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4285 5186 5448 820 Size: 197 926 2342 901 262 1553 Fragments arranged by size: 2342 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 3539 1132 Size: 2407 3774 SspI AAT'ATT Cuts at: 5 52 4105 5 Size: 47 4053 2081 Fragments arranged by size: 4053 2081 47 StuI AGG'CCT Cuts at: 1517 2276 1517 Size: 759 5422 StyI C'CwwG_G Cuts at: 513 1065 2004 3219 3620 513 Size: 552 939 1215 401 3074 Fragments arranged by size: 3074 1215 939 552 401 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2482 4208 4225 4378 4563 5008 325 Size: 1255 902 1726 17 153 185 445 1498 Fragments arranged by size: 1726 1498 1255 902 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2257 4427 6139 Size: 274 80 33 51 1443 280 2170 1712 Cuts at: 6139 96 Size: 138 Fragments arranged by size: 2170 1712 1443 280 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2478 2510 2674 2706 6141 6173 2478 Size: 32 164 32 3435 32 2486 Fragments arranged by size: 3435 2486 164 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6181 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2344 3226 2344 Size: 882 5299 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6181 XmaI C'CCGG_G Cuts at: 1870 3506 1870 Size: 1636 4545 XmnI GAAnn'nnTTC Cuts at: 4310 4310 Size: 6181 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SrfI SwaI