(Linear) (Six-base) MAPSORT of: KIEE2647.seq Check: 3636 from: 1 to: 1476 KIEE2647.orf With 182 enzymes: SgfI * November 11, 2016 16:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 552 1476 Size: 552 924 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 1476 Size: 124 1352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 538 841 1476 Size: 538 303 635 Fragments arranged by size: 635 538 303 AlwNI CAG_nnn'CTG Cuts at: 0 623 1302 1476 Size: 623 679 174 Fragments arranged by size: 679 623 174 ApaI G_GGCC'C Cuts at: 0 763 1476 Size: 763 713 ApaLI G'TGCA_C Cuts at: 0 283 565 1476 Size: 283 282 911 Fragments arranged by size: 911 283 282 AvaI C'yCGr_G Cuts at: 0 7 49 556 1096 1476 Size: 7 42 507 540 380 Fragments arranged by size: 540 507 380 42 7 BanI G'GyrC_C Cuts at: 0 448 817 1236 1476 Size: 448 369 419 240 Fragments arranged by size: 448 419 369 240 BanII G_rGCy'C Cuts at: 0 8 136 557 763 1056 1476 Size: 8 128 421 206 293 420 Fragments arranged by size: 421 420 293 206 128 8 BbeI G_GCGC'C Cuts at: 0 452 1476 Size: 452 1024 BbsI GAAGACnn'nnnn_ Cuts at: 0 611 1476 Size: 611 865 BclI T'GATC_A Cuts at: 0 431 1476 Size: 431 1045 BglI GCCn_nnn'nGGC Cuts at: 0 394 649 758 772 934 943 1021 1168 Size: 394 255 109 14 162 9 78 147 Cuts at: 1168 1476 Size: 308 Fragments arranged by size: 394 308 255 162 147 109 78 14 9 BglII A'GATC_T Cuts at: 0 1322 1476 Size: 1322 154 Bme1580I G_kGCm'C Cuts at: 0 262 287 569 763 1476 Size: 262 25 282 194 713 Fragments arranged by size: 713 282 262 194 25 BsaI GGTCTCn'nnnn_ Cuts at: 0 175 1476 Size: 175 1301 BsaHI Gr'CG_yC Cuts at: 0 449 920 1476 Size: 449 471 556 Fragments arranged by size: 556 471 449 BsaWI w'CCGG_w Cuts at: 0 184 1476 Size: 184 1292 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 473 503 669 699 865 895 1476 Size: 473 30 166 30 166 30 581 Fragments arranged by size: 581 473 166 166 30 30 30 BseYI C'CCAG_C Cuts at: 0 118 756 951 1476 Size: 118 638 195 525 Fragments arranged by size: 638 525 195 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 187 511 610 1476 Size: 45 142 324 99 866 Fragments arranged by size: 866 324 142 99 45 BsiEI CG_ry'CG Cuts at: 0 725 737 908 1163 1172 1476 Size: 725 12 171 255 9 304 Fragments arranged by size: 725 304 255 171 12 9 BsiHKAI G_wGCw'C Cuts at: 0 287 569 1476 Size: 287 282 907 Fragments arranged by size: 907 287 282 BsiWI C'GTAC_G Cuts at: 0 689 1476 Size: 689 787 BsmI GAATG_Cn' Cuts at: 0 282 1476 Size: 282 1194 Bsp1286I G_dGCh'C Cuts at: 0 8 136 262 287 557 569 763 1056 Size: 8 128 126 25 270 12 194 293 Cuts at: 1056 1476 Size: 420 Fragments arranged by size: 420 293 270 194 128 126 25 12 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 530 552 833 1476 Size: 530 22 281 643 Fragments arranged by size: 643 530 281 22 BsrBI CCG'CTC Cuts at: 0 240 1405 1476 Size: 240 1165 71 Fragments arranged by size: 1165 240 71 BsrFI r'CCGG_y Cuts at: 0 679 1361 1476 Size: 679 682 115 Fragments arranged by size: 682 679 115 BstAPI GCAn_nnn'nTGC Cuts at: 0 623 1476 Size: 623 853 BstEII G'GTnAC_C Cuts at: 0 1368 1428 1476 Size: 1368 60 48 Fragments arranged by size: 1368 60 48 BstXI CCAn_nnnn'nTGG Cuts at: 0 885 978 1221 1476 Size: 885 93 243 255 Fragments arranged by size: 885 255 243 93 BstYI r'GATC_y Cuts at: 0 1322 1476 Size: 1322 154 Bsu36I CC'TnA_GG Cuts at: 0 338 1476 Size: 338 1138 BtgI C'CryG_G Cuts at: 0 452 659 701 719 932 1157 1388 1476 Size: 452 207 42 18 213 225 231 88 Fragments arranged by size: 452 231 225 213 207 88 42 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 340 1476 Size: 340 1136 EaeI y'GGCC_r Cuts at: 0 662 722 734 830 905 926 935 1073 Size: 662 60 12 96 75 21 9 138 Cuts at: 1073 1145 1160 1169 1391 1476 Size: 72 15 9 222 85 Fragments arranged by size: 662 222 138 96 85 75 72 60 21 15 12 9 9 EagI C'GGCC_G Cuts at: 0 722 734 905 1160 1169 1476 Size: 722 12 171 255 9 307 Fragments arranged by size: 722 307 255 171 12 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1139 1204 1476 Size: 1139 65 272 Fragments arranged by size: 1139 272 65 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 1476 Size: 124 1352 EcoNI CCTnn'n_nnAGG Cuts at: 0 253 1267 1476 Size: 253 1014 209 Fragments arranged by size: 1014 253 209 EcoO109I rG'GnC_Cy Cuts at: 0 12 32 655 760 785 1061 1451 1476 Size: 12 20 623 105 25 276 390 25 Fragments arranged by size: 623 390 276 105 25 25 20 12 HaeII r_GCGC'y Cuts at: 0 452 584 847 1313 1476 Size: 452 132 263 466 163 Fragments arranged by size: 466 452 263 163 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 179 211 1296 1328 1476 Size: 179 32 1085 32 148 Fragments arranged by size: 1085 179 148 32 32 HincII GTy'rAC Cuts at: 0 348 1476 Size: 348 1128 KasI G'GCGC_C Cuts at: 0 448 1476 Size: 448 1028 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 695 1476 Size: 695 781 MscI TGG'CCA Cuts at: 0 664 832 1476 Size: 664 168 644 Fragments arranged by size: 664 644 168 MspA1I CmG'CkG Cuts at: 0 171 495 548 623 703 721 1140 1150 Size: 171 324 53 75 80 18 419 10 Cuts at: 1150 1159 1390 1425 1476 Size: 9 231 35 51 Fragments arranged by size: 419 324 231 171 80 75 53 51 35 18 10 9 NarI GG'CG_CC Cuts at: 0 449 1476 Size: 449 1027 NotI GC'GGCC_GC Cuts at: 0 722 1476 Size: 722 754 PasI CC'CwG_GG Cuts at: 0 885 1056 1476 Size: 885 171 420 Fragments arranged by size: 885 420 171 PflMI CCAn_nnn'nTGG Cuts at: 0 1470 1476 Size: 1470 6 PfoI T'CCnGG_A Cuts at: 0 1047 1476 Size: 1047 429 PpuMI rG'GwC_Cy Cuts at: 0 1061 1476 Size: 1061 415 PspOMI G'GGCC_C Cuts at: 0 759 1476 Size: 759 717 PstI C_TGCA'G Cuts at: 0 176 527 1155 1476 Size: 176 351 628 321 Fragments arranged by size: 628 351 321 176 PvuII CAG'CTG Cuts at: 0 171 495 623 1476 Size: 171 324 128 853 Fragments arranged by size: 853 324 171 128 SacII CC_GC'GG Cuts at: 0 704 722 1160 1391 1476 Size: 704 18 438 231 85 Fragments arranged by size: 704 438 231 85 18 SbfI CC_TGCA'GG Cuts at: 0 527 1476 Size: 527 949 SexAI A'CCwGG_T Cuts at: 0 1247 1476 Size: 1247 229 SfcI C'TryA_G Cuts at: 0 172 523 1151 1476 Size: 172 351 628 325 Fragments arranged by size: 628 351 325 172 SfiI GGCCn_nnn'nGGCC Cuts at: 0 758 934 943 1168 1476 Size: 758 176 9 225 308 Fragments arranged by size: 758 308 225 176 9 SfoI GGC'GCC Cuts at: 0 450 1476 Size: 450 1026 StuI AGG'CCT Cuts at: 0 271 1476 Size: 271 1205 StyI C'CwwG_G Cuts at: 0 1214 1476 Size: 1214 262 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 477 1476 Size: 477 999 TatI w'GTAC_w Cuts at: 0 252 1476 Size: 252 1224 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 473 505 669 701 1476 Size: 473 32 164 32 775 Fragments arranged by size: 775 473 164 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 339 1221 1476 Size: 339 882 255 Fragments arranged by size: 882 339 255 Enzymes that do cut: AarI AcuI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BclI BglI BglII Bme1580I BsaI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI EaeI EagI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MspA1I NarI NotI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SbfI SexAI SfcI SfiI SfoI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BseRI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI ScaI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI