################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AocI 177 CCTAAGG CC^T[ACGT]AGG AspHI 278 GAGCAC G[AT]GC[AT]^C BbrPI 592 CACGTG CAC^GTG BbvCI 496 CCTCAGC CCTCAGC(-5/-2) BbvI 69 GCTGC GCAGC(8/12) BcnI 1398 CCGGG CC^[GC]GG BfaI 1069 CTAG C^TAG BmyI 278 GAGCAC G[AGT]GC[ACT]^C BsaAI 592 CACGTG [CT]AC^GT[GA] BsiHKAI 278 GAGCAC G[AT]GC[AT]^C BsmBI 1554 GAGACG CGTCTC(1/5) Bsp1286I 278 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 1479 TGTACA T^GTACA BspHI 810 TCATGA T^CATGA BspMI 128 ACCTGC ACCTGC(4/8) BsrGI 1479 TGTACA T^GTACA Bsu36I 177 CCTAAGG CC^T[ACGT]AGG Cac8I 299 GCCAGC GC[ACGT]^[ACGT]GC CfoI 1569 GCGC GCG^C DsaI 920 CCATGG C^C[GA][CT]GG Eco81I 177 CCTAAGG CC^T[ACGT]AGG EcoRI 228 GAATTC G^AATTC EcoT14I 920 CCATGG C^C[AT][AT]GG Fnu4HI 69 GCTGC GC^[ACGT]GC HaeII 1568 GGCGCT [GA]GCGC^[CT] HapII 1398 CCGG C^CGG HgaI 1556 GACGC GACGC(5/10) HhaI 1569 GCGC GCG^C HinP1I 1569 GCGC G^CGC HindIII 1194 AAGCTT A^AGCTT HpaII 1398 CCGG C^CGG ItaI 69 GCTGC GC^[ACGT]GC MaeI 1069 CTAG C^TAG MaeII 593 ACGT A^CGT MspI 1398 CCGG C^CGG MwoI 63 GCTGGGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 1398 CCGGG CC^[GC]GG NcoI 920 CCATGG C^CATGG PflMI 915 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 592 CACGTG CAC^GTG PmlI 592 CACGTG CAC^GTG RcaI 810 TCATGA T^CATGA SexAI 334 ACCAGGT A^CC[AT]GGT SfcI 1515 CTATAG C^T[GA][CT]AG SspBI 1479 TGTACA T^GTACA StuI 800 AGGCCT AGG^CCT StyI 920 CCATGG C^C[AT][AT]GG TseI 69 GCTGC G^C[AT]GC Tsp45I 22 GTGAC ^GT[GC]AC Van91I 915 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflII 823 CTTAAG C^TTAAG 907 CTTAAG AflIII 529 ACATGT A^C[GA][CT]GT 958 ACATGT AlwI 243 GATCC GGATC(4/5) 1259 GATCC BbsI 46 GTCTTC GAAGAC(2/6) 1245 GTCTTC BfrI 823 CTTAAG C^TTAAG 907 CTTAAG BpmI 112 CTGGAG CTGGAG(16/14) 680 CTGGAG BpuAI 46 GTCTTC GAAGAC(2/6) 1245 GTCTTC BsaI 1359 GAGACC GGTCTC(1/5) 338 GGTCTC BsmFI 205 GGGAC GGGAC(10/14) 388 GTCCC BspLU11I 529 ACATGT A^CATGT 958 ACATGT BsrDI 1000 CATTGC GCAATG(2/0) 705 GCAATG BsrI 679 ACTGG ACTGG(1/-1) 847 ACTGG BstYI 242 AGATCC [GA]^GATC[CT] 1258 AGATCC HaeIII 801 GGCC GG^CC 1401 GGCC HphI 382 GGTGA GGTGA(8/7) 1270 GGTGA MflI 242 AGATCC [GA]^GATC[CT] 1258 AGATCC SapI 1134 GAAGAGC GCTCTTC(1/4) 568 GCTCTTC XhoII 242 AGATCC [GA]^GATC[CT] 1258 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I AvaII BsaJI BseRI BsiYI BslI BsmAI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII FokI HinfI Ksp632I MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TfiI Tru9I Tsp509I TspRI XmnI