(Circular) (Six-base) MAPSORT of: pFN21AB7827.seq Check: 9097 from: 1 to: 6319 pFN21AB7827 6319 bp With 182 enzymes: SgfI * March 28, 2012 12:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2110 2458 3130 4382 5766 6034 1632 Size: 478 348 672 1252 1384 268 1917 Fragments arranged by size: 1917 1384 1252 672 478 348 268 AflII C'TTAA_G Cuts at: 820 1017 2828 2912 820 Size: 197 1811 84 4227 Fragments arranged by size: 4227 1811 197 84 AflIII A'CryG_T Cuts at: 1203 2534 2963 5218 6195 1203 Size: 1331 429 2255 977 1327 Fragments arranged by size: 2255 1331 1327 977 429 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 3273 1166 Size: 2107 4212 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6319 AlwNI CAG_nnn'CTG Cuts at: 4062 5634 4062 Size: 1572 4747 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 2233 2639 3238 3628 3854 4006 6115 1493 Size: 740 406 599 390 226 152 2109 1697 Fragments arranged by size: 2109 1697 740 599 406 390 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 3644 1870 Size: 73 1701 4545 Fragments arranged by size: 4545 1701 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3640 5095 618 Size: 325 217 627 1853 1455 1842 Fragments arranged by size: 1853 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3638 1391 Size: 2247 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 3243 928 Size: 971 145 1199 4004 Fragments arranged by size: 4004 1199 971 145 BbvCI CC'TCA_GC Cuts at: 2502 2502 Size: 6319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4930 136 Size: 107 122 71 631 605 3258 1525 Fragments arranged by size: 3258 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 3736 3736 Size: 6319 Bme1580I G_kGCm'C Cuts at: 1790 4380 5536 1790 Size: 2590 1156 2573 Fragments arranged by size: 2590 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2862 4050 5008 Size: 416 53 83 399 24 1445 1188 958 Cuts at: 5008 442 Size: 1753 Fragments arranged by size: 1753 1445 1188 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2627 2627 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2706 4979 1239 Size: 899 568 2273 2579 Fragments arranged by size: 2579 2273 899 568 Bpu10I CC'TnA_GC Cuts at: 2502 2502 Size: 6319 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3394 4444 5309 5607 3394 Size: 1050 865 298 4106 Fragments arranged by size: 4106 1050 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2349 3358 4982 882 Size: 617 850 1009 1624 2219 Fragments arranged by size: 2219 1624 1009 850 617 BsaAI yAC'GTr Cuts at: 493 1204 2599 4110 493 Size: 711 1395 1511 2702 Fragments arranged by size: 2702 1511 1395 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4126 4508 5143 275 Size: 53 83 186 936 2593 382 635 1451 Fragments arranged by size: 2593 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4752 5150 5424 5571 5982 4752 Size: 398 274 147 411 5089 Fragments arranged by size: 5089 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6094 Fragments arranged by size: 6094 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2078 3311 1186 Size: 889 3 1233 4194 Fragments arranged by size: 4194 1233 889 3 BseYI C'CCAG_C Cuts at: 2068 2823 5522 6018 2068 Size: 755 2699 496 2369 Fragments arranged by size: 2699 2369 755 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5746 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2287 4380 4465 5536 2287 Size: 2093 85 1071 3070 Fragments arranged by size: 3070 2093 1071 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 BsmBI CGTCTCn'nnnn_ Cuts at: 3553 3553 Size: 6319 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2287 3638 4380 4465 5536 1391 Size: 399 497 1351 742 85 1071 2174 Fragments arranged by size: 2174 1351 1071 742 497 399 85 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 2815 4208 5938 2815 Size: 1393 1730 3196 Fragments arranged by size: 3196 1730 1393 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2142 3674 844 Size: 1298 1532 3489 Fragments arranged by size: 3489 1532 1298 BsrBI CCG'CTC Cuts at: 1394 1823 4206 1394 Size: 429 2383 3507 Fragments arranged by size: 3507 2383 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2717 3004 4814 4988 65 Size: 1183 1469 287 1810 174 1396 Fragments arranged by size: 1810 1469 1396 1183 287 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4963 5150 1700 Size: 110 3153 187 2869 Fragments arranged by size: 3153 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3484 96 Size: 3388 2931 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6319 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstYI r'GATC_y Cuts at: 1972 2247 3263 3649 4016 4401 4418 5870 5956 Size: 275 1016 386 367 385 17 1452 86 Cuts at: 5956 5968 6314 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1016 386 385 367 346 275 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2183 2183 Size: 6319 BtgI C'CryG_G Cuts at: 513 1065 2925 513 Size: 552 1860 3907 Fragments arranged by size: 3907 1860 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 2031 3053 3436 3835 4629 4649 2031 Size: 1022 383 399 794 20 3701 Fragments arranged by size: 3701 1022 794 399 383 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3766 4655 6284 Size: 54 1425 50 275 18 1936 889 1629 Cuts at: 6284 8 Size: 43 Fragments arranged by size: 1936 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 2514 2580 3134 4249 1353 Size: 933 228 66 554 1115 3423 Fragments arranged by size: 3423 1115 933 554 228 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2138 2458 2706 3130 4382 4979 Size: 393 478 28 320 248 424 1252 597 Cuts at: 4979 5766 6034 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1252 787 597 478 424 393 320 268 248 28 EcoRI G'AATT_C Cuts at: 2233 3628 4006 2233 Size: 1395 378 4546 Fragments arranged by size: 4546 1395 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3592 3592 Size: 6319 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 3577 5466 1678 Size: 1899 1889 2531 Fragments arranged by size: 2531 1899 1889 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2232 2249 2264 2281 2381 Size: 32 135 32 660 17 15 17 100 Cuts at: 2381 2413 2965 2997 4960 4992 5034 5066 1373 Size: 32 552 32 1963 32 42 32 2626 Fragments arranged by size: 2626 1963 660 552 135 100 42 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 3199 748 Size: 2451 3868 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2241 5433 5617 1305 Size: 936 3192 184 2007 Fragments arranged by size: 3192 2007 936 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4497 Fragments arranged by size: 4497 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2701 2869 2953 3273 3289 3457 3541 Size: 648 1535 168 84 320 16 168 84 Cuts at: 3541 4277 4636 4795 518 Size: 736 359 159 2042 Fragments arranged by size: 2042 1535 736 648 359 320 168 168 159 84 84 16 MspA1I CmG'CkG Cuts at: 1606 3735 4412 5560 5805 1606 Size: 2129 677 1148 245 2120 Fragments arranged by size: 2129 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2925 513 Size: 552 1860 3907 Fragments arranged by size: 3907 1860 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 2538 2799 2967 3677 5222 1132 Size: 97 1309 261 168 710 1545 2229 Fragments arranged by size: 2229 1545 1309 710 261 168 97 PasI CC'CwG_GG Cuts at: 1348 2449 1348 Size: 1101 5218 PciI A'CATG_T Cuts at: 2534 2963 5218 2534 Size: 429 2255 3635 Fragments arranged by size: 3635 2255 429 PflMI CCAn_nnn'nTGG Cuts at: 1108 2926 1108 Size: 1818 4501 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PmlI CAC'GTG Cuts at: 2599 2599 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2123 2155 4388 4420 5901 5933 2123 Size: 32 2233 32 1481 32 2509 Fragments arranged by size: 2509 2233 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3084 3372 3889 3084 Size: 288 517 5514 Fragments arranged by size: 5514 517 288 PstI C_TGCA'G Cuts at: 830 3671 830 Size: 2841 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SapI GCTCTTCn'nnn_ Cuts at: 2580 3134 2580 Size: 554 5765 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SexAI A'CCwGG_T Cuts at: 2339 2339 Size: 6319 SfcI C'TryA_G Cuts at: 826 1045 3520 3667 4802 5483 5674 826 Size: 219 2475 147 1135 681 191 1471 Fragments arranged by size: 2475 1471 1135 681 219 191 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 3646 1872 Size: 1774 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2828 2912 3409 4423 5324 5586 Size: 197 926 885 84 497 1014 901 262 Cuts at: 5586 820 Size: 1553 Fragments arranged by size: 1553 1014 926 901 885 497 262 197 84 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 2807 1517 Size: 1290 5029 StyI C'CwwG_G Cuts at: 513 1065 2925 3758 513 Size: 552 1860 833 3074 Fragments arranged by size: 3074 1860 833 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4346 4363 4516 4701 5146 325 Size: 1255 2766 17 153 185 445 1498 Fragments arranged by size: 2766 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3484 4565 6277 Size: 274 80 33 51 1443 1507 1081 1712 Cuts at: 6277 96 Size: 138 Fragments arranged by size: 1712 1507 1443 1081 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6279 6311 6279 Size: 32 6287 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 3644 1870 Size: 1774 4545 XmnI GAAnn'nnTTC Cuts at: 2696 2723 2807 2864 3143 4448 2696 Size: 27 84 57 279 1305 4567 Fragments arranged by size: 4567 1305 279 84 57 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI