Restriction map for
pFN21AE5416
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BamHI BbrPI BbvCI BcgI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KpnI Ksp632I MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 175 GACGTC GACGT^C
AccI 502 GTCTAC GT^[AC][GT]AC
AcsI 589 GAATTC [GA]^AATT[CT]
AfaI 458 GTAC GT^AC
AlwNI 85 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 949 GGGCCC GGGCC^C
ApoI 589 GAATTC [GA]^AATT[CT]
AspI 552 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 903 TGGCCA TGG^CCA
BbeI 895 GGCGCC GGCGC^C
BbsI 147 GAAGAC GAAGAC(2/6)
BciVI 933 GTATCC GTATCC(6/5)
BglI 190 GCCGTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 147 GAAGAC GAAGAC(2/6)
BsiEI 470 CGGTCG CG[GA][CT]^CG
BsrDI 318 GCAATG GCAATG(2/0)
BsrFI 468 GCCGGT [GA]^CCGG[CT]
BssHII 161 GCGCGC G^CGCGC
BssSI 856 CACGAG CACGAG(-5/-1)
BstYI 282 GGATCT [GA]^GATC[CT]
Cfr10I 468 GCCGGT [GA]^CCGG[CT]
DrdI 520 GACGGCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 937 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 589 GAATTC G^AATTC
HincII 885 GTTGAC GT[CT]^[GA]AC
HindII 885 GTTGAC GT[CT]^[GA]AC
KasI 895 GGCGCC G^GCGCC
KspI 273 CCGCGG CCGC^GG
MflI 282 GGATCT [GA]^GATC[CT]
MluNI 903 TGGCCA TGG^CCA
MscI 903 TGGCCA TGG^CCA
MslI 738 CACGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 895 GGCGCC GG^CGCC
PflFI 552 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 32 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 652 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 458 GTAC GT^AC
SacII 273 CCGCGG CCGC^GG
SfaNI 128 GATGC GCATC(5/9)
SfoI 895 GGCGCC GGC^GCC
SmaI 866 CCCGGG CCC^GGG
StuI 860 AGGCCT AGG^CCT
TfiI 701 GAATC G^A[AT]TC
Tth111I 552 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 32 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 986 CCACAGCTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 282 GGATCT [GA]^GATC[CT]
XmaCI 866 CCCGGG C^CCGGG
XmaI 866 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 175 GACGTC G[GA]^CG[CT]C
895 GGCGCC
AlwI 282 GGATC GGATC(4/5)
330 GGATC
AvaI 599 CTCGGG C^[CT]CG[GA]G
866 CCCGGG
BanI 22 GGTGCC G^G[CT][GA]CC
895 GGCGCC
BsaHI 175 GACGTC G[GA]^CG[CT]C
895 GGCGCC
BsaI 205 GAGACC GGTCTC(1/5)
789 GAGACC
BseRI 77 GAGGAG GAGGAG(10/8)
874 GAGGAG
BsmFI 252 GGGAC GGGAC(10/14)
654 GTCCC
BsoBI 599 CTCGGG C^[CT]CG[GA]G
866 CCCGGG
BspMI 19 GCAGGT ACCTGC(4/8)
379 GCAGGT
BstXI 404 CCAACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
737 CCACGACTGTGG
EaeI 903 TGGCCA [CT]^GGCC[GA]
1013 TGGCCG
Hin1I 175 GACGTC G[GA]^CG[CT]C
895 GGCGCC
MaeII 176 ACGT A^CGT
527 ACGT
MboII 147 GAAGA GAAGA(8/7)
285 TCTTC
PleI 254 GACTC GAGTC(4/5)
834 GACTC
PstI 44 CTGCAG CTGCA^G
313 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI SfcI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I