(Circular) (Six-base) MAPSORT of: pFN21AE5416.seq Check: 7544 from: 1 to: 5743 pFN21AE5416 5743 bp With 182 enzymes: SgfI * September 8, 2016 14:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2015 2015 Size: 5743 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2184 3553 278 Size: 53 83 186 936 648 1369 2468 Fragments arranged by size: 2468 1369 936 648 186 83 53 AccI GT'mk_AC Cuts at: 1937 2508 3086 1937 Size: 571 578 4594 Fragments arranged by size: 4594 578 571 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3806 5190 5458 1632 Size: 2174 1384 268 1917 Fragments arranged by size: 2174 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 2747 1166 Size: 1581 4162 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2958 1184 Size: 1774 3969 AlwNI CAG_nnn'CTG Cuts at: 2095 3486 5058 2095 Size: 1391 1572 2780 Fragments arranged by size: 2780 1572 1391 ApaI G_GGCC'C Cuts at: 2958 2958 Size: 5743 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 2594 3052 3278 3430 5539 1493 Size: 1101 458 226 152 2109 1697 Fragments arranged by size: 2109 1697 1101 458 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2604 2871 3068 1870 Size: 73 661 267 197 4545 Fragments arranged by size: 4545 661 267 197 73 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2900 3064 4519 618 Size: 325 217 627 240 873 164 1455 1842 Fragments arranged by size: 1842 1455 873 627 325 240 217 164 BanII G_rGCy'C Cuts at: 1391 2742 2886 2958 3062 1391 Size: 1351 144 72 104 4072 Fragments arranged by size: 4072 1351 144 104 72 BbeI G_GCGC'C Cuts at: 2904 2904 Size: 5743 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2159 928 Size: 971 260 4512 Fragments arranged by size: 4512 971 260 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2949 3635 4845 2949 Size: 686 1210 3847 Fragments arranged by size: 3847 1210 686 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2201 4354 136 Size: 107 122 71 631 605 529 2153 1525 Fragments arranged by size: 2153 1525 631 605 529 122 107 71 BglII A'GATC_T Cuts at: 5738 5738 Size: 5743 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 2032 2958 3804 4960 1790 Size: 242 926 846 1156 2573 Fragments arranged by size: 2573 1156 926 846 242 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2783 3474 4432 Size: 416 53 83 399 24 1366 691 958 Cuts at: 4432 442 Size: 1753 Fragments arranged by size: 1753 1366 958 691 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2718 2916 4403 1239 Size: 878 601 198 1487 2579 Fragments arranged by size: 2579 1487 878 601 198 Bpu10I CC'TnA_GC Cuts at: 2331 2441 2331 Size: 110 5633 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3868 4733 5031 3868 Size: 865 298 4580 Fragments arranged by size: 4580 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2204 2788 4406 882 Size: 617 705 584 1618 2219 Fragments arranged by size: 2219 1618 705 617 584 BsaAI yAC'GTr Cuts at: 493 1204 3534 493 Size: 711 2330 2702 Fragments arranged by size: 2702 2330 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2181 2901 3550 3932 Size: 53 83 186 936 648 720 649 382 Cuts at: 3932 4567 275 Size: 635 1451 Fragments arranged by size: 1451 936 720 649 648 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 2852 2873 2882 Size: 30 165 30 717 30 702 21 9 Cuts at: 2882 2903 1178 Size: 21 4018 Fragments arranged by size: 4018 717 702 165 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2097 2894 1186 Size: 911 797 4035 Fragments arranged by size: 4035 911 797 BseYI C'CCAG_C Cuts at: 2958 4946 5442 2958 Size: 1988 496 3259 Fragments arranged by size: 3259 1988 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 2170 2584 1163 Size: 573 399 35 414 4322 Fragments arranged by size: 4322 573 414 399 35 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2478 3193 3954 4982 1540 Size: 275 186 477 715 761 1028 2301 Fragments arranged by size: 2301 1028 761 715 477 275 186 BsiHKAI G_wGCw'C Cuts at: 3804 3889 4960 3804 Size: 85 1071 4587 Fragments arranged by size: 4587 1071 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2742 2886 2958 3062 3804 3889 Size: 399 242 710 144 72 104 742 85 Cuts at: 3889 4960 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 710 399 242 144 104 85 72 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2015 2375 3098 844 Size: 1171 360 723 3489 Fragments arranged by size: 3489 1171 723 360 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2330 4238 4412 65 Size: 1183 1082 1908 174 1396 Fragments arranged by size: 1908 1396 1183 1082 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2473 4387 4574 1700 Size: 110 663 1914 187 2869 Fragments arranged by size: 2869 1914 663 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5743 BssHII G'CGCG_C Cuts at: 1925 2166 1925 Size: 241 5502 BssSI C'ACGA_G Cuts at: 2861 3803 4815 2861 Size: 942 1012 3789 Fragments arranged by size: 3789 1012 942 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5743 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstXI CCAn_nnnn'nTGG Cuts at: 2416 2749 2416 Size: 333 5410 BstYI r'GATC_y Cuts at: 1972 2287 3073 3440 3825 3842 5294 5380 5392 Size: 315 786 367 385 17 1452 86 12 Cuts at: 5392 5738 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 786 385 367 346 315 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2196 2218 2278 3021 513 Size: 552 939 192 22 60 743 3235 Fragments arranged by size: 3235 939 743 552 192 60 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2451 487 Size: 683 56 1225 3779 Fragments arranged by size: 3779 1225 683 56 BtsI GCAGTG_nn' Cuts at: 2244 3259 4053 4073 2244 Size: 1015 794 20 3914 Fragments arranged by size: 3914 1015 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2531 4750 809 Size: 507 1215 2219 1802 Fragments arranged by size: 2219 1802 1215 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2908 3018 3190 Size: 54 1425 50 275 18 1078 110 172 Cuts at: 3190 4079 5708 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1078 889 275 172 110 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3673 1353 Size: 2320 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4330 4704 4850 387 Size: 1064 138 2741 374 146 1280 Fragments arranged by size: 2741 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2718 2916 3806 4403 5190 5458 Size: 393 485 601 198 890 597 787 268 Cuts at: 5458 1239 Size: 1524 Fragments arranged by size: 1524 890 787 601 597 485 393 268 198 EcoNI CCTnn'n_nnAGG Cuts at: 2946 2946 Size: 5743 EcoO109I rG'GnC_Cy Cuts at: 2092 2658 2954 2955 2092 Size: 566 296 1 4880 Fragments arranged by size: 4880 566 296 1 EcoRI G'AATT_C Cuts at: 2594 3052 3430 2594 Size: 458 378 4907 Fragments arranged by size: 4907 458 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2536 2710 2536 Size: 174 5569 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 2375 2828 2904 4890 1678 Size: 697 453 76 1986 2531 Fragments arranged by size: 2531 1986 697 453 76 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 4384 4416 4458 Size: 32 135 32 548 32 2232 32 42 Cuts at: 4458 4490 1373 Size: 32 2626 Fragments arranged by size: 2626 2232 548 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3087 3333 669 Size: 988 281 954 195 246 3079 Fragments arranged by size: 3079 988 954 281 246 195 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KasI G'GCGC_C Cuts at: 2900 2900 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4857 5041 1305 Size: 3552 184 2007 Fragments arranged by size: 3552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2910 10 Size: 54 1425 343 1078 2843 Fragments arranged by size: 2843 1425 1078 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2747 3701 4060 4219 518 Size: 648 1581 954 359 159 2042 Fragments arranged by size: 2042 1581 954 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2109 2280 2317 2430 2664 2906 2996 3159 Size: 503 171 37 113 234 242 90 163 Cuts at: 3159 3836 4984 5229 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 503 245 242 234 171 163 113 90 37 NarI GG'CG_CC Cuts at: 2901 2901 Size: 5743 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4252 Fragments arranged by size: 4252 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 3101 4646 1132 Size: 97 1872 1545 2229 Fragments arranged by size: 2229 1872 1545 97 PasI CC'CwG_GG Cuts at: 1348 2624 1348 Size: 1276 4467 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2043 1108 Size: 935 4808 PfoI T'CCnGG_A Cuts at: 2586 2586 Size: 5743 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2658 2658 Size: 5743 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PspOMI G'GGCC_C Cuts at: 2954 2954 Size: 5743 PstI C_TGCA'G Cuts at: 830 2053 2322 3095 830 Size: 1223 269 773 3478 Fragments arranged by size: 3478 1223 773 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 PvuII CAG'CTG Cuts at: 2109 2317 2430 2996 2109 Size: 208 113 566 4856 Fragments arranged by size: 4856 566 208 113 SacII CC_GC'GG Cuts at: 2281 2281 Size: 5743 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SanDI GG'GwC_CC Cuts at: 2658 2658 Size: 5743 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 3991 5703 3991 Size: 1712 4031 SfcI C'TryA_G Cuts at: 826 1045 2049 2104 2318 2425 3091 4226 4907 Size: 219 1004 55 214 107 666 1135 681 Cuts at: 4907 5098 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1004 681 666 219 214 191 107 55 SfoI GGC'GCC Cuts at: 2902 2902 Size: 5743 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 2873 3070 1872 Size: 1001 197 4545 Fragments arranged by size: 4545 1001 197 SmlI C'TyrA_G Cuts at: 820 1017 1943 3847 4748 5010 820 Size: 197 926 1904 901 262 1553 Fragments arranged by size: 1904 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 2867 1517 Size: 1350 4393 StyI C'CwwG_G Cuts at: 513 1065 2004 3182 513 Size: 552 939 1178 3074 Fragments arranged by size: 3074 1178 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3770 3787 3940 4125 4570 325 Size: 1255 2190 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3989 5701 96 Size: 274 80 33 51 1443 2012 1712 138 Fragments arranged by size: 2012 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2011 2043 2703 2735 2852 2884 5703 5735 2011 Size: 32 660 32 117 32 2819 32 2019 Fragments arranged by size: 2819 2019 660 117 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2560 1316 Size: 1244 4499 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2998 2998 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 2871 3068 1870 Size: 1001 197 4545 Fragments arranged by size: 4545 1001 197 XmnI GAAnn'nnTTC Cuts at: 3872 3872 Size: 5743 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2182 3551 276 Size: 53 83 186 936 648 1369 2468 Fragments arranged by size: 2468 1369 936 648 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI