Restriction map for
pFN21AE5416
(Linear) (Six-base) MAPSORT of: KIEE5416.seq Check: 136 from: 1 to: 1038
KIEE5416.orf
With 182 enzymes: SgfI *
September 8, 2016 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 10 1038
Size: 10 1028
AatII G_ACGT'C
Cuts at: 0 179 1038
Size: 179 859
AccI GT'mk_AC
Cuts at: 0 503 1038
Size: 503 535
AleI CACnn'nnGTG
Cuts at: 0 742 1038
Size: 742 296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 953 1038
Size: 953 85
AlwNI CAG_nnn'CTG
Cuts at: 0 90 1038
Size: 90 948
ApaI G_GGCC'C
Cuts at: 0 953 1038
Size: 953 85
ApoI r'AATT_y
Cuts at: 0 589 1038
Size: 589 449
AvaI C'yCGr_G
Cuts at: 0 599 866 1038
Size: 599 267 172
Fragments arranged by size:
599 267 172
BanI G'GyrC_C
Cuts at: 0 22 895 1038
Size: 22 873 143
Fragments arranged by size:
873 143 22
BanII G_rGCy'C
Cuts at: 0 737 881 953 1038
Size: 737 144 72 85
Fragments arranged by size:
737 144 85 72
BbeI G_GCGC'C
Cuts at: 0 899 1038
Size: 899 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 154 1038
Size: 154 884
BciVI GTATCCnnnnn_n'
Cuts at: 0 944 1038
Size: 944 94
BglI GCCn_nnn'nGGC
Cuts at: 0 196 1038
Size: 196 842
Bme1580I G_kGCm'C
Cuts at: 0 27 953 1038
Size: 27 926 85
Fragments arranged by size:
926 85 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 778 1038
Size: 778 260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 713 911 1038
Size: 112 601 198 127
Fragments arranged by size:
601 198 127 112
Bpu10I CC'TnA_GC
Cuts at: 0 326 436 1038
Size: 326 110 602
Fragments arranged by size:
602 326 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 199 783 1038
Size: 199 584 255
Fragments arranged by size:
584 255 199
BsaHI Gr'CG_yC
Cuts at: 0 176 896 1038
Size: 176 720 142
Fragments arranged by size:
720 176 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 847 868 877 898 1038
Size: 115 30 702 21 9 21 140
Fragments arranged by size:
702 140 115 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 92 889 1038
Size: 92 797 149
Fragments arranged by size:
797 149 92
BseYI C'CCAG_C
Cuts at: 0 953 1038
Size: 953 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 165 579 1038
Size: 130 35 414 459
Fragments arranged by size:
459 414 130 35
BsiEI CG_ry'CG
Cuts at: 0 473 1038
Size: 473 565
Bsp1286I G_dGCh'C
Cuts at: 0 27 737 881 953 1038
Size: 27 710 144 72 85
Fragments arranged by size:
710 144 85 72 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 10 370 1038
Size: 10 360 668
Fragments arranged by size:
668 360 10
BsrDI GCAATG_nn'
Cuts at: 0 325 1038
Size: 325 713
BsrFI r'CCGG_y
Cuts at: 0 468 1038
Size: 468 570
BssHII G'CGCG_C
Cuts at: 0 161 1038
Size: 161 877
BssSI C'ACGA_G
Cuts at: 0 856 1038
Size: 856 182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 744 1038
Size: 411 333 294
Fragments arranged by size:
411 333 294
BstYI r'GATC_y
Cuts at: 0 282 1038
Size: 282 756
BtgI C'CryG_G
Cuts at: 0 191 213 273 1016 1038
Size: 191 22 60 743 22
Fragments arranged by size:
743 191 60 22 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 446 1038
Size: 446 592
BtsI GCAGTG_nn'
Cuts at: 0 239 1038
Size: 239 799
DrdI GACnn_nn'nnGTC
Cuts at: 0 526 1038
Size: 526 512
EaeI y'GGCC_r
Cuts at: 0 903 1013 1038
Size: 903 110 25
Fragments arranged by size:
903 110 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 713 911 1038
Size: 112 601 198 127
Fragments arranged by size:
601 198 127 112
EcoNI CCTnn'n_nnAGG
Cuts at: 0 941 1038
Size: 941 97
EcoO109I rG'GnC_Cy
Cuts at: 0 87 653 949 950 1038
Size: 87 566 296 1 88
Fragments arranged by size:
566 296 88 87 1
EcoRI G'AATT_C
Cuts at: 0 589 1038
Size: 589 449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 531 705 1038
Size: 531 174 333
Fragments arranged by size:
531 333 174
HaeII r_GCGC'y
Cuts at: 0 370 823 899 1038
Size: 370 453 76 139
Fragments arranged by size:
453 370 139 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 1038
Size: 115 32 891
Fragments arranged by size:
891 115 32
HincII GTy'rAC
Cuts at: 0 887 1038
Size: 887 151
KasI G'GCGC_C
Cuts at: 0 895 1038
Size: 895 143
MscI TGG'CCA
Cuts at: 0 905 1038
Size: 905 133
MslI CAynn'nnrTG
Cuts at: 0 742 1038
Size: 742 296
MspA1I CmG'CkG
Cuts at: 0 104 275 312 425 659 901 991 1038
Size: 104 171 37 113 234 242 90 47
Fragments arranged by size:
242 234 171 113 104 90 47 37
NarI GG'CG_CC
Cuts at: 0 896 1038
Size: 896 142
PasI CC'CwG_GG
Cuts at: 0 619 1038
Size: 619 419
PflMI CCAn_nnn'nTGG
Cuts at: 0 38 1038
Size: 38 1000
PfoI T'CCnGG_A
Cuts at: 0 581 1038
Size: 581 457
PpuMI rG'GwC_Cy
Cuts at: 0 653 1038
Size: 653 385
PspOMI G'GGCC_C
Cuts at: 0 949 1038
Size: 949 89
PstI C_TGCA'G
Cuts at: 0 48 317 1038
Size: 48 269 721
Fragments arranged by size:
721 269 48
PvuII CAG'CTG
Cuts at: 0 104 312 425 991 1038
Size: 104 208 113 566 47
Fragments arranged by size:
566 208 113 104 47
SacII CC_GC'GG
Cuts at: 0 276 1038
Size: 276 762
SanDI GG'GwC_CC
Cuts at: 0 653 1038
Size: 653 385
SfcI C'TryA_G
Cuts at: 0 44 99 313 420 1038
Size: 44 55 214 107 618
Fragments arranged by size:
618 214 107 55 44
SfoI GGC'GCC
Cuts at: 0 897 1038
Size: 897 141
SmaI CCC'GGG
Cuts at: 0 868 1038
Size: 868 170
StuI AGG'CCT
Cuts at: 0 862 1038
Size: 862 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 6 38 698 730 847 879 1038
Size: 6 32 660 32 117 32 159
Fragments arranged by size:
660 159 117 32 32 32 6
Tth111I GACn'n_nGTC
Cuts at: 0 555 1038
Size: 555 483
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 993 1038
Size: 993 45
XmaI C'CCGG_G
Cuts at: 0 866 1038
Size: 866 172
ZraI GAC'GTC
Cuts at: 0 177 1038
Size: 177 861
Enzymes that do cut:
AarI AatII AccI AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BciVI BglI Bme1580I
BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI Bsp1286I BspMI BsrDI BsrFI BssHII BssSI
BstXI BstYI BtgI BtgZI BtsI DrdI EaeI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MscI MslI MspA1I NarI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacII SanDI SfcI SfoI SmaI
StuI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrGI BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EarI
EciI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StyI
SwaI TaqII TatI XbaI XhoI XmnI