################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BalI BamHI BbrPI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BseAI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HgaI HincII HindII HpaI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 273 GACGTC GACGT^C Aor51HI 155 AGCGCT AGC^GCT AviII 335 TGCGCA TGC^GCA BbsI 454 GTCTTC GAAGAC(2/6) BcgI 56 CGACCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 93 CCGGG CC^[GC]GG BlpI 45 GCTGAGC GC^T[ACGT]AGC Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC BpuAI 454 GTCTTC GAAGAC(2/6) BsaWI 82 TCCGGT [AT]^CCGG[AT] BseRI 532 GAGGAG GAGGAG(10/8) BsgI 86 GTGCAG GTGCAG(16/14) BsiEI 449 CGGCCG CG[GA][CT]^CG BsiWI 201 CGTACG C^GTACG BsmFI 276 GTCCC GGGAC(10/14) BstXI 473 CCACCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 45 GCTGAGC GC^T[ACGT]AGC DdeI 46 CTGAG C^T[ACGT]AG EagI 449 CGGCCG C^GGCCG EclXI 449 CGGCCG C^GGCCG Eco47III 155 AGCGCT AGC^GCT Eco52I 449 CGGCCG C^GGCCG EcoT14I 341 CCTTGG C^C[AT][AT]GG FspI 335 TGCGCA TGC^GCA HindIII 352 AAGCTT A^AGCTT NciI 93 CCGGG CC^[GC]GG PleI 346 GAGTC GAGTC(4/5) PstI 538 CTGCAG CTGCA^G ScaI 215 AGTACT AGT^ACT SfcI 538 CTGCAG C^T[GA][CT]AG StyI 341 CCTTGG C^C[AT][AT]GG TfiI 75 GATTC G^A[AT]TC Tsp45I 271 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 115 CGCG CG^CG 117 CGCG AspHI 135 GAGCAC G[AT]GC[AT]^C 358 GTGCTC AvaII 15 GGACC G^G[AT]CC 147 GGACC BanI 110 GGCGCC G^G[CT][GA]CC 313 GGCGCC BanII 378 GAGCCC G[GA]GC[CT]^C 444 GGGCTC BbeI 110 GGCGCC GGCGC^C 313 GGCGCC BsiHKAI 135 GAGCAC G[AT]GC[AT]^C 358 GTGCTC BsrBI 48 GAGCGG CCGCTC(-3/-3) 106 GAGCGG BstF5I 123 CATCC GGATG(2/0) 255 CATCC BstUI 115 CGCG CG^CG 117 CGCG EaeI 366 TGGCCG [CT]^GGCC[GA] 449 CGGCCG FauI 170 CCCGC CCCGC(4/6) 442 GCGGG FokI 123 CATCC GGATG(9/13) 255 CATCC HinfI 75 GATTC G^A[ACGT]TC 346 GAGTC HphI 270 GGTGA GGTGA(8/7) 375 GGTGA KasI 110 GGCGCC G^GCGCC 313 GGCGCC MaeII 209 ACGT A^CGT 274 ACGT MaeIII 271 GTGAC ^GT[ACGT]AC 320 GTTAC MboII 425 TCTTC GAAGA(8/7) 455 TCTTC MvnI 115 CGCG CG^CG 117 CGCG NarI 110 GGCGCC GG^CGCC 313 GGCGCC SfoI 110 GGCGCC GGC^GCC 313 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AcyI AfaI AluI BbvI BmyI BpmI BsaHI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I EcoRII Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MnlI MslI MspI MvaI MwoI NlaIV RsaI Sau96I ScrFI SfaNI TseI