(Linear) (Six-base) MAPSORT of: KIEE1666.seq Check: 7301 from: 1 to: 555 KIEE1666.orf With 182 enzymes: SgfI * July 15, 2010 18:44 .. AatII G_ACGT'C Cuts at: 0 277 555 Size: 277 278 AfeI AGC'GCT Cuts at: 0 157 555 Size: 157 398 AleI CACnn'nnGTG Cuts at: 0 142 478 555 Size: 142 336 77 Fragments arranged by size: 336 142 77 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 44 76 410 555 Size: 44 32 334 145 Fragments arranged by size: 334 145 44 32 BanI G'GyrC_C Cuts at: 0 110 313 555 Size: 110 203 242 Fragments arranged by size: 242 203 110 BanII G_rGCy'C Cuts at: 0 382 448 555 Size: 382 66 107 Fragments arranged by size: 382 107 66 BbeI G_GCGC'C Cuts at: 0 114 317 555 Size: 114 203 238 Fragments arranged by size: 238 203 114 BbsI GAAGACnn'nnnn_ Cuts at: 0 447 555 Size: 447 108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 45 79 555 Size: 45 34 476 Fragments arranged by size: 476 45 34 BlpI GC'TnA_GC Cuts at: 0 46 555 Size: 46 509 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 153 425 555 Size: 124 29 272 130 Fragments arranged by size: 272 130 124 29 BsaHI Gr'CG_yC Cuts at: 0 111 274 314 555 Size: 111 163 40 241 Fragments arranged by size: 241 163 111 40 BsaWI w'CCGG_w Cuts at: 0 82 555 Size: 82 473 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 547 555 Size: 547 8 BseYI C'CCAG_C Cuts at: 0 381 465 555 Size: 381 84 90 Fragments arranged by size: 381 90 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 107 555 Size: 107 448 BsiEI CG_ry'CG Cuts at: 0 452 555 Size: 452 103 BsiHKAI G_wGCw'C Cuts at: 0 139 362 555 Size: 139 223 193 Fragments arranged by size: 223 193 139 BsiWI C'GTAC_G Cuts at: 0 201 555 Size: 201 354 Bsp1286I G_dGCh'C Cuts at: 0 139 362 382 448 555 Size: 139 223 20 66 107 Fragments arranged by size: 223 139 107 66 20 BsrBI CCG'CTC Cuts at: 0 50 108 555 Size: 50 58 447 Fragments arranged by size: 447 58 50 BstXI CCAn_nnnn'nTGG Cuts at: 0 480 555 Size: 480 75 EaeI y'GGCC_r Cuts at: 0 366 449 555 Size: 366 83 106 Fragments arranged by size: 366 106 83 EagI C'GGCC_G Cuts at: 0 449 555 Size: 449 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 52 555 Size: 52 503 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 153 425 555 Size: 124 29 272 130 Fragments arranged by size: 272 130 124 29 FspI TGC'GCA Cuts at: 0 337 555 Size: 337 218 HaeII r_GCGC'y Cuts at: 0 114 159 198 317 555 Size: 114 45 39 119 238 Fragments arranged by size: 238 119 114 45 39 HindIII A'AGCT_T Cuts at: 0 352 555 Size: 352 203 KasI G'GCGC_C Cuts at: 0 110 313 555 Size: 110 203 242 Fragments arranged by size: 242 203 110 MslI CAynn'nnrTG Cuts at: 0 142 392 478 555 Size: 142 250 86 77 Fragments arranged by size: 250 142 86 77 NarI GG'CG_CC Cuts at: 0 111 314 555 Size: 111 203 241 Fragments arranged by size: 241 203 111 PstI C_TGCA'G Cuts at: 0 542 555 Size: 542 13 ScaI AGT'ACT Cuts at: 0 217 555 Size: 217 338 SfcI C'TryA_G Cuts at: 0 538 555 Size: 538 17 SfoI GGC'GCC Cuts at: 0 112 315 555 Size: 112 203 240 Fragments arranged by size: 240 203 112 StyI C'CwwG_G Cuts at: 0 341 555 Size: 341 214 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 450 555 Size: 450 105 TatI w'GTAC_w Cuts at: 0 215 260 555 Size: 215 45 295 Fragments arranged by size: 295 215 45 ZraI GAC'GTC Cuts at: 0 275 555 Size: 275 280 Enzymes that do cut: AatII AfeI AleI AlfI BanI BanII BbeI BbsI BcgI BlpI BpmI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BsrBI BstXI EaeI EagI EciI Eco57MI FspI HaeII HindIII KasI MslI NarI PstI ScaI SfcI SfoI StyI TaqII TatI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI Hin4I HincII HpaI KpnI MfeI MluI MmeI MscI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI