################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tsp509I TspRI Tth111I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccI 70 GTCTAC GT^[AC][GT]AC AcyI 145 GACGCC G[GA]^CG[CT]C AfaI 266 GTAC GT^AC AlwI 446 GGATC GGATC(4/5) AlwNI 91 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG BbrPI 222 CACGTG CAC^GTG BbsI 142 GAAGAC GAAGAC(2/6) BclI 272 TGATCA T^GATCA BpuAI 142 GAAGAC GAAGAC(2/6) BsaHI 145 GACGCC G[GA]^CG[CT]C BseRI 49 CTCCTC GAGGAG(10/8) DdeI 233 CTGAG C^T[ACGT]AG DraIII 192 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 52 CTCTTC CTCTTC(1/4) FauI 162 CCCGC CCCGC(4/6) FbaI 272 TGATCA T^GATCA Hin1I 145 GACGCC G[GA]^CG[CT]C Ksp632I 52 CTCTTC CTCTTC(1/4) MaeIII 190 GTCAC ^GT[ACGT]AC MslI 306 CATATACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 664 CAGCTG C[AC]G^C[GT]G PflMI 630 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 222 CACGTG CAC^GTG PmlI 222 CACGTG CAC^GTG PvuII 664 CAGCTG CAG^CTG RsaI 266 GTAC GT^AC SexAI 193 ACCTGGT A^CC[AT]GGT StuI 567 AGGCCT AGG^CCT TfiI 428 GAATC G^A[AT]TC Tsp45I 190 GTCAC ^GT[GC]AC Van91I 630 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 389 CCAGGCCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 221 ACACGT A^C[GA][CT]GT 311 ACGTGT ApaI 78 GGGCCC GGGCC^C 243 GGGCCC BanII 78 GGGCCC G[GA]GC[CT]^C 243 GGGCCC BpmI 616 CTCCAG CTGGAG(16/14) 667 CTGGAG BsaAI 222 CACGTG [CT]AC^GT[GA] 310 TACGTG BsmFI 478 GGGAC GGGAC(10/14) 112 GTCCC DpnII 273 GATC ^GATC 447 GATC DsaI 210 CCATGG C^C[GA][CT]GG 297 CCATGG EcoT14I 210 CCATGG C^C[AT][AT]GG 297 CCATGG HphI 129 GGTGA GGTGA(8/7) 191 TCACC MboI 273 GATC ^GATC 447 GATC NcoI 210 CCATGG C^CATGG 297 CCATGG NdeII 273 GATC ^GATC 447 GATC PleI 17 GAGTC GAGTC(4/5) 337 GAGTC PpuMI 110 AGGTCCC [GA]G^G[AT]CC[CT] 254 AGGACCC Sau3AI 273 GATC ^GATC 447 GATC SmaI 246 CCCGGG CCC^GGG 608 CCCGGG StyI 210 CCATGG C^C[AT][AT]GG 297 CCATGG XmaCI 246 CCCGGG C^CCGGG 608 CCCGGG XmaI 246 CCCGGG C^CCGGG 608 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AccII AciI AluI AvaI AvaII BanI BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MaeII MboII MnlI MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TseI