Restriction map for
pFN21AE6640
(Linear) (Six-base) MAPSORT of: KIEE6640.seq Check: 9786 from: 1 to: 678
KIEE6640.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AccI GT'mk_AC
Cuts at: 0 71 678
Size: 71 607
AflIII A'CryG_T
Cuts at: 0 221 311 678
Size: 221 90 367
Fragments arranged by size:
367 221 90
AlwNI CAG_nnn'CTG
Cuts at: 0 96 678
Size: 96 582
ApaI G_GGCC'C
Cuts at: 0 82 247 678
Size: 82 165 431
Fragments arranged by size:
431 165 82
AvaI C'yCGr_G
Cuts at: 0 246 520 608 678
Size: 246 274 88 70
Fragments arranged by size:
274 246 88 70
BanI G'GyrC_C
Cuts at: 0 86 358 415 471 622 678
Size: 86 272 57 56 151 56
Fragments arranged by size:
272 151 86 57 56 56
BanII G_rGCy'C
Cuts at: 0 82 247 678
Size: 82 165 431
Fragments arranged by size:
431 165 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 149 678
Size: 149 529
BclI T'GATC_A
Cuts at: 0 272 678
Size: 272 406
BglI GCCn_nnn'nGGC
Cuts at: 0 302 390 620 678
Size: 302 88 230 58
Fragments arranged by size:
302 230 88 58
Bme1580I G_kGCm'C
Cuts at: 0 82 247 418 625 678
Size: 82 165 171 207 53
Fragments arranged by size:
207 171 165 82 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 621 678
Size: 621 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 678
Size: 601 77
BsaAI yAC'GTr
Cuts at: 0 224 312 678
Size: 224 88 366
Fragments arranged by size:
366 224 88
BsaHI Gr'CG_yC
Cuts at: 0 146 678
Size: 146 532
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 571 601 678
Size: 571 30 77
Fragments arranged by size:
571 77 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 678
Size: 40 638
BseYI C'CCAG_C
Cuts at: 0 114 678
Size: 114 564
Bsp1286I G_dGCh'C
Cuts at: 0 82 247 418 625 678
Size: 82 165 171 207 53
Fragments arranged by size:
207 171 165 82 53
BtgI C'CryG_G
Cuts at: 0 210 297 678
Size: 210 87 381
Fragments arranged by size:
381 210 87
DraIII CAC_nnn'GTG
Cuts at: 0 197 678
Size: 197 481
EarI CTCTTCn'nnn_
Cuts at: 0 58 678
Size: 58 620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 678
Size: 601 77
EcoO109I rG'GnC_Cy
Cuts at: 0 79 93 111 255 392 612 678
Size: 79 14 18 144 137 220 66
Fragments arranged by size:
220 144 137 79 66 18 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 571 603 678
Size: 571 32 75
Fragments arranged by size:
571 75 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 678
Size: 506 172
MslI CAynn'nnrTG
Cuts at: 0 310 678
Size: 310 368
MspA1I CmG'CkG
Cuts at: 0 666 678
Size: 666 12
NcoI C'CATG_G
Cuts at: 0 210 297 678
Size: 210 87 381
Fragments arranged by size:
381 210 87
PasI CC'CwG_GG
Cuts at: 0 82 678
Size: 82 596
PflMI CCAn_nnn'nTGG
Cuts at: 0 636 678
Size: 636 42
PfoI T'CCnGG_A
Cuts at: 0 12 323 398 678
Size: 12 311 75 280
Fragments arranged by size:
311 280 75 12
PmlI CAC'GTG
Cuts at: 0 224 678
Size: 224 454
PpuMI rG'GwC_Cy
Cuts at: 0 111 255 678
Size: 111 144 423
Fragments arranged by size:
423 144 111
PspOMI G'GGCC_C
Cuts at: 0 78 243 678
Size: 78 165 435
Fragments arranged by size:
435 165 78
PvuII CAG'CTG
Cuts at: 0 666 678
Size: 666 12
SexAI A'CCwGG_T
Cuts at: 0 193 678
Size: 193 485
SmaI CCC'GGG
Cuts at: 0 248 610 678
Size: 248 362 68
Fragments arranged by size:
362 248 68
SrfI GCCC'GGGC
Cuts at: 0 248 678
Size: 248 430
StuI AGG'CCT
Cuts at: 0 569 678
Size: 569 109
StyI C'CwwG_G
Cuts at: 0 210 297 678
Size: 210 87 381
Fragments arranged by size:
381 210 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 74 678
Size: 74 604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 678
Size: 396 282
XmaI C'CCGG_G
Cuts at: 0 246 608 678
Size: 246 362 70
Fragments arranged by size:
362 246 70
Enzymes that do cut:
AccI AflIII AlwNI ApaI AvaI BanI BanII BbsI
BclI BglI Bme1580I BplI BpmI BsaAI BsaHI BsaXI
BseRI BseYI Bsp1286I BtgI DraIII EarI Eco57MI EcoO109I
Hin4I MmeI MslI MspA1I NcoI PasI PflMI PfoI
PmlI PpuMI PspOMI PvuII SexAI SmaI SrfI StuI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI
BsaI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI