################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI Sse8387I SspBI StuI StyI SwaI Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflIII 369 ACACGT A^C[GA][CT]GT AluI 741 AGCT AG^CT AlwI 327 GATCC GGATC(4/5) ApaI 277 GGGCCC GGGCC^C AspI 682 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 667 GGTGCC G^G[CT][GA]CC BfaI 157 CTAG C^TAG BsaAI 418 TACGTA [CT]AC^GT[GA] BsaWI 468 TCCGGT [AT]^CCGG[AT] BsiEI 291 CGGTCG CG[GA][CT]^CG BsmI 35 GCATTC GAATGC(1/-1) BspHI 761 TCATGA T^CATGA BspMI 86 ACCTGC ACCTGC(4/8) BstF5I 782 GGATG GGATG(2/0) CfoI 614 GCGC GCG^C DpnII 327 GATC ^GATC FauI 280 CCCGC CCCGC(4/6) FokI 782 GGATG GGATG(9/13) HaeII 613 AGCGCC [GA]GCGC^[CT] HaeIII 278 GGCC GG^CC HapII 469 CCGG C^CGG HgaI 349 GACGC GACGC(5/10) HhaI 614 GCGC GCG^C HinP1I 614 GCGC G^CGC HincII 503 GTCAAC GT[CT]^[GA]AC HindII 503 GTCAAC GT[CT]^[GA]AC HpaII 469 CCGG C^CGG MaeI 157 CTAG C^TAG MboI 327 GATC ^GATC MboII 397 GAAGA GAAGA(8/7) MspI 469 CCGG C^CGG NdeII 327 GATC ^GATC NspI 219 GCATGC [GA]CATG^[CT] PflFI 682 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 747 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 740 CAGCTG CAG^CTG RcaI 761 TCATGA T^CATGA Sau3AI 327 GATC ^GATC ScaI 771 AGTACT AGT^ACT SfaNI 758 GCATC GCATC(5/9) SnaBI 418 TACGTA TAC^GTA SphI 219 GCATGC GCATG^C SspI 109 AATATT AAT^ATT Tth111I 682 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 177 CCAGAATCTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BanII 49 GGGCTC G[GA]GC[CT]^C 277 GGGCCC BbvI 573 GCTGC GCAGC(8/12) 576 GCTGC BsmAI 298 GAGAC GTCTC(1/5) 511 GAGAC MseI 73 TTAA T^TAA 496 TTAA MspA1I 740 CAGCTG C[AC]G^C[GT]G 749 CCGCTG NlaIII 220 CATG CATG^ 762 CATG TaqI 231 TCGA T^CGA 294 TCGA TfiI 180 GAATC G^A[AT]TC 356 GAATC Tru9I 73 TTAA T^TAA 496 TTAA TseI 573 GCTGC G^C[AT]GC 576 GCTGC Tsp509I 234 AATT ^AATT 413 AATT TthHB8I 231 TCGA T^CGA 294 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AfaI AvaII BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HinfI HphI ItaI MaeII MaeIII MnlI MvaI MwoI NlaIV PleI PpuMI RsaI Sau96I ScrFI Tsp45I TspRI