Restriction map for
pFN21AE6511
##################################
# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanII
BbeI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HphI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I XbaI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 306 CCCGGG C^[CT]CG[GA]G
BbsI 206 GTCTTC GAAGAC(2/6)
BbvCI 4 GCTGAGG CCTCAGC(-5/-2)
BpmI 475 CTGGAG CTGGAG(16/14)
BpuAI 206 GTCTTC GAAGAC(2/6)
BsmI 77 GCATTC GAATGC(1/-1)
BsoBI 306 CCCGGG C^[CT]CG[GA]G
BspMI 185 ACCTGC ACCTGC(4/8)
BstAPI 396 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI 5 CTGAG C^T[ACGT]AG
EaeI 258 TGGCCG [CT]^GGCC[GA]
EarI 61 CTCTTC CTCTTC(1/4)
Ksp632I 61 CTCTTC CTCTTC(1/4)
PflMI 315 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 420 CTACAG C^T[GA][CT]AG
SmaI 306 CCCGGG CCC^GGG
TspRI 423 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 315 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 246 CCAGGACAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 306 CCCGGG C^CCGGG
XmaI 306 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 10 GTGCAC G^TGCAC
469 GTGCAC
AlwNI 397 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
452 CAGCTGCTG
ApaLI 10 GTGCAC G^TGCAC
469 GTGCAC
AspHI 10 GTGCAC G[AT]GC[AT]^C
469 GTGCAC
AvaII 170 GGTCC G^G[AT]CC
242 GGTCC
BanI 310 GGCACC G^G[CT][GA]CC
463 GGCACC
BbrPI 13 CACGTG CAC^GTG
333 CACGTG
BsaAI 13 CACGTG [CT]AC^GT[GA]
333 CACGTG
BsgI 187 CTGCAC GTGCAG(16/14)
445 CTGCAC
BsiEI 432 CGACCG CG[GA][CT]^CG
504 CGACCG
BsiHKAI 10 GTGCAC G[AT]GC[AT]^C
469 GTGCAC
BsrI 167 ACTGG ACTGG(1/-1)
299 CCAGT
FauI 144 CCCGC CCCGC(4/6)
117 GCGGG
HapII 222 CCGG C^CGG
307 CCGG
HpaII 222 CCGG C^CGG
307 CCGG
MaeII 14 ACGT A^CGT
334 ACGT
MboII 62 TCTTC GAAGA(8/7)
207 TCTTC
MspI 222 CCGG C^CGG
307 CCGG
PmaCI 13 CACGTG CAC^GTG
333 CACGTG
PmlI 13 CACGTG CAC^GTG
333 CACGTG
TaqI 182 TCGA T^CGA
503 TCGA
TthHB8I 182 TCGA T^CGA
503 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI Cac8I Cfr13I DpnII
EcoRII Fnu4HI HaeIII ItaI MboI
MnlI MspA1I MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI TseI