################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaI AvrII BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsgI BsiEI BsmI BsoBI BspEI BspLU11I BsrBI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccII 367 CGCG CG^CG AlwNI 715 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 188 AGCGCT AGC^GCT ApaI 220 GGGCCC GGGCC^C AspI 322 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 605 TGCGCA TGC^GCA BalI 1331 TGGCCA TGG^CCA BamHI 777 GGATCC G^GATCC BbeI 493 GGCGCC GGCGC^C BbrPI 1429 CACGTG CAC^GTG BbsI 328 GTCTTC GAAGAC(2/6) BbvCI 283 GCTGAGG CCTCAGC(-5/-2) BfaI 616 CTAG C^TAG BpmI 730 CTGGAG CTGGAG(16/14) BpuAI 328 GTCTTC GAAGAC(2/6) BsaAI 1429 CACGTG [CT]AC^GT[GA] BsiWI 769 CGTACG C^GTACG BsmBI 1088 GAGACG CGTCTC(1/5) Bsp1407I 161 TGTACA T^GTACA BspDI 502 ATCGAT AT^CGAT BsrDI 116 CATTGC GCAATG(2/0) BsrFI 1013 ACCGGC [GA]^CCGG[CT] BsrGI 161 TGTACA T^GTACA BssHII 366 GCGCGC G^CGCGC BssSI 1069 CACGAG CACGAG(-5/-1) BstUI 367 CGCG CG^CG Cfr10I 1013 ACCGGC [GA]^CCGG[CT] ClaI 502 ATCGAT AT^CGAT EaeI 1331 TGGCCA [CT]^GGCC[GA] Eco47III 188 AGCGCT AGC^GCT EcoNI 1152 CCTCCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 974 CCAAGG C^C[AT][AT]GG FspI 605 TGCGCA TGC^GCA HgaI 472 GACGC GACGC(5/10) HincII 636 GTCAAC GT[CT]^[GA]AC HindII 636 GTCAAC GT[CT]^[GA]AC KasI 493 GGCGCC G^GCGCC MaeI 616 CTAG C^TAG MluNI 1331 TGGCCA TGG^CCA MscI 1331 TGGCCA TGG^CCA MseI 1268 TTAA T^TAA MspA1I 294 CAGCGG C[AC]G^C[GT]G MvnI 367 CGCG CG^CG NarI 493 GGCGCC GG^CGCC NspI 821 GCATGC [GA]CATG^[CT] PflFI 322 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1429 CACGTG CAC^GTG PmlI 1429 CACGTG CAC^GTG PpuMI 105 GGGACCC [GA]G^G[AT]CC[CT] SacI 1342 GAGCTC GAGCT^C SapI 81 GAAGAGC GCTCTTC(1/4) SexAI 869 ACCTGGT A^CC[AT]GGT SfoI 493 GGCGCC GGC^GCC SmlI 1384 CTCAAG C^T[CT][GA]AG SphI 821 GCATGC GCATG^C SspBI 161 TGTACA T^GTACA StyI 974 CCAAGG C^C[AT][AT]GG TfiI 620 GAATC G^A[AT]TC Tru9I 1268 TTAA T^TAA Tsp509I 784 AATT ^AATT Tth111I 322 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 472 GACGCC G[GA]^CG[CT]C 493 GGCGCC AflIII 1415 ACGTGT A^C[GA][CT]GT 1428 ACACGT Asp700I 888 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1311 GAACTGTTTC AspHI 757 GAGCAC G[AT]GC[AT]^C 1342 GAGCTC BsaHI 472 GACGCC G[GA]^CG[CT]C 493 GGCGCC BsaI 910 GAGACC GGTCTC(1/5) 1199 GAGACC BsiHKAI 757 GAGCAC G[AT]GC[AT]^C 1342 GAGCTC BspHI 173 TCATGA T^CATGA 950 TCATGA BspMI 137 ACCTGC ACCTGC(4/8) 686 ACCTGC BstYI 777 GGATCC [GA]^GATC[CT] 1073 AGATCC DsaI 644 CCACGG C^C[GA][CT]GG 790 CCACGG Hin1I 472 GACGCC G[GA]^CG[CT]C 493 GGCGCC MaeIII 597 GTCAC ^GT[ACGT]AC 1398 GTCAC MflI 777 GGATCC [GA]^GATC[CT] 1073 AGATCC PleI 805 GACTC GAGTC(4/5) 1118 GAGTC RcaI 173 TCATGA T^CATGA 950 TCATGA StuI 707 AGGCCT AGG^CCT 1326 AGGCCT Tsp45I 597 GTCAC ^GT[GC]AC 1398 GTCAC XhoII 777 GGATCC [GA]^GATC[CT] 1073 AGATCC XmnI 888 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1311 GAACTGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwI AocI AvaII BanI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I