(Linear) (Six-base) MAPSORT of: KIEE5649.seq Check: 7005 from: 1 to: 1482 KIEE5649.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 695 1482 Size: 695 787 AfeI AGC'GCT Cuts at: 0 190 1482 Size: 190 1292 AflIII A'CryG_T Cuts at: 0 1415 1428 1482 Size: 1415 13 54 Fragments arranged by size: 1415 54 13 AleI CACnn'nnGTG Cuts at: 0 1168 1482 Size: 1168 314 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 943 1482 Size: 943 539 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 574 606 1482 Size: 574 32 876 Fragments arranged by size: 876 574 32 AlwNI CAG_nnn'CTG Cuts at: 0 720 1482 Size: 720 762 ApaI G_GGCC'C Cuts at: 0 224 1482 Size: 224 1258 BamHI G'GATC_C Cuts at: 0 777 1482 Size: 777 705 BanI G'GyrC_C Cuts at: 0 493 904 1162 1482 Size: 493 411 258 320 Fragments arranged by size: 493 411 320 258 BanII G_rGCy'C Cuts at: 0 224 1189 1346 1482 Size: 224 965 157 136 Fragments arranged by size: 965 224 157 136 BbeI G_GCGC'C Cuts at: 0 497 1482 Size: 497 985 BbsI GAAGACnn'nnnn_ Cuts at: 0 321 1482 Size: 321 1161 BbvCI CC'TCA_GC Cuts at: 0 284 1482 Size: 284 1198 Bme1580I G_kGCm'C Cuts at: 0 224 487 907 1165 1292 1482 Size: 224 263 420 258 127 190 Fragments arranged by size: 420 263 258 224 190 127 BmgBI CAC'GTC Cuts at: 0 635 1092 1416 1482 Size: 635 457 324 66 Fragments arranged by size: 635 457 324 66 BmrI ACTGGGnnnn_n' Cuts at: 0 333 1199 1389 1482 Size: 333 866 190 93 Fragments arranged by size: 866 333 190 93 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 684 1482 Size: 684 798 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 751 1482 Size: 751 731 Bpu10I CC'TnA_GC Cuts at: 0 284 520 1482 Size: 284 236 962 Fragments arranged by size: 962 284 236 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1369 1482 Size: 1369 113 BsaI GGTCTCn'nnnn_ Cuts at: 0 904 1193 1482 Size: 904 289 289 Fragments arranged by size: 904 289 289 BsaAI yAC'GTr Cuts at: 0 1431 1482 Size: 1431 51 BsaHI Gr'CG_yC Cuts at: 0 473 494 1482 Size: 473 21 988 Fragments arranged by size: 988 473 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 301 451 466 844 847 1000 1482 Size: 301 150 15 378 3 153 482 Fragments arranged by size: 482 378 301 153 150 15 3 BseYI C'CCAG_C Cuts at: 0 1182 1238 1482 Size: 1182 56 244 Fragments arranged by size: 1182 244 56 BsiHKAI G_wGCw'C Cuts at: 0 761 1346 1482 Size: 761 585 136 Fragments arranged by size: 761 585 136 BsiWI C'GTAC_G Cuts at: 0 769 1482 Size: 769 713 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1082 1482 Size: 1082 400 Bsp1286I G_dGCh'C Cuts at: 0 224 487 761 907 1165 1189 1292 1346 Size: 224 263 274 146 258 24 103 54 Cuts at: 1346 1482 Size: 136 Fragments arranged by size: 274 263 258 224 146 136 103 54 24 BspHI T'CATG_A Cuts at: 0 173 950 1482 Size: 173 777 532 Fragments arranged by size: 777 532 173 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 146 695 1482 Size: 146 549 787 Fragments arranged by size: 787 549 146 BsrDI GCAATG_nn' Cuts at: 0 115 1482 Size: 115 1367 BsrFI r'CCGG_y Cuts at: 0 1013 1482 Size: 1013 469 BsrGI T'GTAC_A Cuts at: 0 161 1482 Size: 161 1321 BssHII G'CGCG_C Cuts at: 0 366 1482 Size: 366 1116 BssSI C'ACGA_G Cuts at: 0 1069 1482 Size: 1069 413 BstXI CCAn_nnnn'nTGG Cuts at: 0 216 753 1084 1107 1431 1482 Size: 216 537 331 23 324 51 Fragments arranged by size: 537 331 324 216 51 23 BstYI r'GATC_y Cuts at: 0 777 1073 1482 Size: 777 296 409 Fragments arranged by size: 777 409 296 Bsu36I CC'TnA_GG Cuts at: 0 9 1157 1403 1482 Size: 9 1148 246 79 Fragments arranged by size: 1148 246 79 9 BtgI C'CryG_G Cuts at: 0 644 790 1482 Size: 644 146 692 Fragments arranged by size: 692 644 146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 378 1482 Size: 378 1104 BtsI GCAGTG_nn' Cuts at: 0 1067 1482 Size: 1067 415 ClaI AT'CG_AT Cuts at: 0 503 1482 Size: 503 979 EaeI y'GGCC_r Cuts at: 0 1331 1482 Size: 1331 151 EarI CTCTTCn'nnn_ Cuts at: 0 76 675 898 1267 1357 1482 Size: 76 599 223 369 90 125 Fragments arranged by size: 599 369 223 125 90 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1369 1482 Size: 1369 113 EcoICRI GAG'CTC Cuts at: 0 1344 1482 Size: 1344 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 751 1482 Size: 751 731 EcoNI CCTnn'n_nnAGG Cuts at: 0 1156 1482 Size: 1156 326 EcoO109I rG'GnC_Cy Cuts at: 0 106 220 221 1482 Size: 106 114 1 1261 Fragments arranged by size: 1261 114 106 1 FspI TGC'GCA Cuts at: 0 607 1482 Size: 607 875 HaeII r_GCGC'y Cuts at: 0 192 387 497 1482 Size: 192 195 110 985 Fragments arranged by size: 985 195 192 110 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 580 612 1079 1111 1482 Size: 580 32 467 32 371 Fragments arranged by size: 580 467 371 32 32 HincII GTy'rAC Cuts at: 0 638 1482 Size: 638 844 KasI G'GCGC_C Cuts at: 0 493 1482 Size: 493 989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1241 1482 Size: 1241 241 MscI TGG'CCA Cuts at: 0 1333 1482 Size: 1333 149 MslI CAynn'nnrTG Cuts at: 0 820 1168 1366 1414 1482 Size: 820 348 198 48 68 Fragments arranged by size: 820 348 198 68 48 MspA1I CmG'CkG Cuts at: 0 296 1482 Size: 296 1186 NarI GG'CG_CC Cuts at: 0 494 1482 Size: 494 988 NspI r_CATG'y Cuts at: 0 825 1482 Size: 825 657 PfoI T'CCnGG_A Cuts at: 0 102 446 1482 Size: 102 344 1036 Fragments arranged by size: 1036 344 102 PmlI CAC'GTG Cuts at: 0 1431 1482 Size: 1431 51 PpuMI rG'GwC_Cy Cuts at: 0 106 1482 Size: 106 1376 PspOMI G'GGCC_C Cuts at: 0 220 1482 Size: 220 1262 SacI G_AGCT'C Cuts at: 0 1346 1482 Size: 1346 136 SanDI GG'GwC_CC Cuts at: 0 106 1482 Size: 106 1376 SapI GCTCTTCn'nnn_ Cuts at: 0 76 1482 Size: 76 1406 SexAI A'CCwGG_T Cuts at: 0 869 1482 Size: 869 613 SfoI GGC'GCC Cuts at: 0 495 1482 Size: 495 987 SmlI C'TyrA_G Cuts at: 0 1384 1482 Size: 1384 98 SphI G_CATG'C Cuts at: 0 825 1482 Size: 825 657 StuI AGG'CCT Cuts at: 0 709 1328 1482 Size: 709 619 154 Fragments arranged by size: 709 619 154 StyI C'CwwG_G Cuts at: 0 974 1482 Size: 974 508 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 974 1482 Size: 974 508 TatI w'GTAC_w Cuts at: 0 161 1482 Size: 161 1321 Tth111I GACn'n_nGTC Cuts at: 0 325 1482 Size: 325 1157 XmnI GAAnn'nnTTC Cuts at: 0 892 1315 1482 Size: 892 423 167 Fragments arranged by size: 892 423 167 Enzymes that do cut: AarI AfeI AflIII AleI AlfI AloI AlwNI ApaI BamHI BanI BanII BbeI BbsI BbvCI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NspI PfoI PmlI PpuMI PspOMI SacI SanDI SapI SexAI SfoI SmlI SphI StuI StyI TaqII TatI Tth111I XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AgeI AhdI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI BsaBI BsaWI BsaXI BsgI BsiEI BsmI BspEI BsrBI BstAPI BstBI BstEII BstZ17I CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI XbaI XcmI XhoI XmaI ZraI