################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AvrII BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BseAI BsiEI BsiWI Bsp1407I BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 1144 GTCTAC GT^[AC][GT]AC AcyI 1967 GGCGCC G[GA]^CG[CT]C AfaI 92 GTAC GT^AC Alw44I 1282 GTGCAC G^TGCAC ApaLI 1282 GTGCAC G^TGCAC Asp700I 55 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1362 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1967 GGCGCC GGCGC^C BbrPI 1354 CACGTG CAC^GTG BglI 1822 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1182 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1967 GGCGCC G[GA]^CG[CT]C BsaWI 178 ACCGGA [AT]^CCGG[AT] BsmBI 1567 CGTCTC CGTCTC(1/5) BspDI 350 ATCGAT AT^CGAT ClaI 350 ATCGAT AT^CGAT EcoNI 1752 CCTCGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 1967 GGCGCC G[GA]^CG[CT]C KasI 1967 GGCGCC G^GCGCC KspI 1383 CCGCGG CCGC^GG MamI 1182 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 1430 TTAA T^TAA NaeI 1827 GCCGGC GCC^GGC NarI 1967 GGCGCC GG^CGCC NcoI 1742 CCATGG C^CATGG NgoMIV 1827 GCCGGC G^CCGGC PflFI 1362 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1354 CACGTG CAC^GTG PmlI 1354 CACGTG CAC^GTG RsaI 92 GTAC GT^AC SacI 1099 GAGCTC GAGCT^C SacII 1383 CCGCGG CCGC^GG SbfI 1765 CCTGCAGG CCTGCA^GG SfoI 1967 GGCGCC GGC^GCC Sse8387I 1765 CCTGCAGG CCTGCA^GG StuI 872 AGGCCT AGG^CCT TfiI 86 GATTC G^A[AT]TC Tru9I 1430 TTAA T^TAA Tsp509I 610 AATT ^AATT Tth111I 1362 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 55 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 993 CGCG CG^CG 1384 CGCG AlwNI 464 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 2002 CAGGAACTG AocI 557 CCTCAGG CC^T[ACGT]AGG 568 CCTGAGG AviII 236 TGCGCA TGC^GCA 1067 TGCGCA BamHI 116 GGATCC G^GATCC 252 GGATCC BbsI 140 GTCTTC GAAGAC(2/6) 211 GTCTTC BpuAI 140 GTCTTC GAAGAC(2/6) 211 GTCTTC BsaAI 1042 TACGTG [CT]AC^GT[GA] 1354 CACGTG BsaI 175 GAGACC GGTCTC(1/5) 2068 GGTCTC BsmI 527 GAATGC GAATGC(1/-1) 936 GAATGC BsrBI 147 GAGCGG CCGCTC(-3/-3) 1504 GAGCGG BstEII 382 GGTTACC G^GT[ACGT]ACC 1128 GGTCACC BstPI 382 GGTTACC G^GT[ACGT]ACC 1128 GGTCACC BstUI 993 CGCG CG^CG 1384 CGCG Bsu36I 557 CCTCAGG CC^T[ACGT]AGG 568 CCTGAGG DrdI 202 GACCCTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1084 GACCTCCTTGTC Eco81I 557 CCTCAGG CC^T[ACGT]AGG 568 CCTGAGG EcoO65I 382 GGTTACC G^GT[ACGT]ACC 1128 GGTCACC EcoT14I 501 CCTTGG C^C[AT][AT]GG 1742 CCATGG FspI 236 TGCGCA TGC^GCA 1067 TGCGCA MvnI 993 CGCG CG^CG 1384 CGCG PvuII 676 CAGCTG CAG^CTG 1801 CAGCTG SapI 1263 GCTCTTC GCTCTTC(1/4) 1557 GCTCTTC SmaI 49 CCCGGG CCC^GGG 734 CCCGGG StyI 501 CCTTGG C^C[AT][AT]GG 1742 CCATGG XmaCI 49 CCCGGG C^CCGGG 734 CCCGGG XmaI 49 CCCGGG C^CCGGG 734 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AluI AlwI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII