Restriction map for
pFN21AE4201
(Circular) (Six-base) MAPSORT of: pFN21AE4201.seq Check: 7722 from: 1 to: 6826
pFN21AE4201 6826 bp
With 182 enzymes: SgfI *
March 28, 2012 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3764 3764
Size: 6826
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4636 278
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3150 4169 1937
Size: 1213 1019 4594
Fragments arranged by size:
4594 1213 1019
Acc65I G'GTAC_C
Cuts at: 4147 4147
Size: 6826
AclI AA'CG_TT
Cuts at: 1563 4953 5326 1563
Size: 3390 373 3063
Fragments arranged by size:
3390 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3825 4889 6273 6541 1632
Size: 2193 1064 1384 268 1917
Fragments arranged by size:
2193 1917 1384 1064 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6629
AflIII A'CryG_T
Cuts at: 1203 5725 6702 1203
Size: 4522 977 1327
Fragments arranged by size:
4522 1327 977
AgeI A'CCGG_T
Cuts at: 5657 5657
Size: 6826
AhdI GACnn_n'nnGTC
Cuts at: 5555 5555
Size: 6826
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2510 1184
Size: 1326 5500
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 6794
AlwNI CAG_nnn'CTG
Cuts at: 2474 4012 4569 6141 2474
Size: 1538 557 1572 3159
Fragments arranged by size:
3159 1572 1538 557
ApaLI G'TGCA_C
Cuts at: 3287 4883 6039 3287
Size: 1596 1156 4074
Fragments arranged by size:
4074 1596 1156
ApoI r'AATT_y
Cuts at: 1493 4135 4361 4513 6622 1493
Size: 2642 226 152 2109 1697
Fragments arranged by size:
2642 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5380 160
Size: 872 4348 1606
Fragments arranged by size:
4348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2739 3256 4151 1870
Size: 73 111 685 517 895 4545
Fragments arranged by size:
4545 895 685 517 111 73
BamHI G'GATC_C
Cuts at: 2121 2257 4156 4523 2121
Size: 136 1899 367 4424
Fragments arranged by size:
4424 1899 367 136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2866 3203 3544 3972 4147
Size: 325 217 627 1079 337 341 428 175
Cuts at: 4147 5602 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1079 627 428 341 337 325
217 175
BanII G_rGCy'C
Cuts at: 1391 2313 2818 3108 3257 3662 3947 4145 1391
Size: 922 505 290 149 405 285 198 4072
Fragments arranged by size:
4072 922 505 405 290 285 198 149
BbeI G_GCGC'C
Cuts at: 3976 3976
Size: 6826
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2138 2209 928
Size: 971 239 71 5545
Fragments arranged by size:
5545 971 239 71
BbvCI CC'TCA_GC
Cuts at: 2448 3264 3963 2448
Size: 816 699 5311
Fragments arranged by size:
5311 816 699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5017 5051 1903
Size: 34 3080 34 3678
Fragments arranged by size:
3678 3080 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4718 5928 4718
Size: 1210 5616
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3833 5437 136
Size: 107 122 71 631 605 2161 1604 1525
Fragments arranged by size:
2161 1604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6821 6821
Size: 6826
BlpI GC'TnA_GC
Cuts at: 4243 4243
Size: 6826
Bme1580I G_kGCm'C
Cuts at: 1790 3291 3549 4887 6043 1790
Size: 1501 258 1338 1156 2573
Fragments arranged by size:
2573 1501 1338 1156 258
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6826
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2034 2293 2405
Size: 416 53 83 399 24 617 259 112
Cuts at: 2405 2632 3543 3594 4557 5515 442
Size: 227 911 51 963 958 1753
Fragments arranged by size:
1753 963 958 911 617 416 399 259
227 112 83 53 51 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 3470 3668 3895 4051 5486 1239
Size: 886 1345 198 227 156 1435 2579
Fragments arranged by size:
2579 1435 1345 886 227 198 156
Bpu10I CC'TnA_GC
Cuts at: 2448 3211 3264 3963 2448
Size: 763 53 699 5311
Fragments arranged by size:
5311 763 699 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4951 5816 6114 4951
Size: 865 298 5663
Fragments arranged by size:
5663 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2174 4079 5489 882
Size: 617 675 1905 1410 2219
Fragments arranged by size:
2219 1905 1410 675 617
BsaAI yAC'GTr
Cuts at: 493 1204 3049 3361 4617 493
Size: 711 1845 312 1256 2702
Fragments arranged by size:
2702 1845 1256 711 312
BsaBI GATnn'nnATC
Cuts at: 3191 6820 3191
Size: 3629 3197
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3973 4633 5015 5650
Size: 53 83 186 936 2440 660 382 635
Cuts at: 5650 275
Size: 1451
Fragments arranged by size:
2440 1451 936 660 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2183 5259 5657 5931 6078 6489 2183
Size: 3076 398 274 147 411 2520
Fragments arranged by size:
3076 2520 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2867 2897 3020 3050 3893
Size: 30 165 30 1464 30 123 30 843
Cuts at: 3893 3923 1178
Size: 30 4081
Fragments arranged by size:
4081 1464 843 165 123 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2307 2360 2591 3005 1186
Size: 1090 31 53 231 414 5007
Fragments arranged by size:
5007 1090 414 231 53 31
BseYI C'CCAG_C
Cuts at: 2524 3118 3405 3866 6029 6525 2524
Size: 594 287 461 2163 496 2825
Fragments arranged by size:
2825 2163 594 496 461 287
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2996 3077 3254 1163
Size: 573 1260 81 177 4735
Fragments arranged by size:
4735 1260 573 177 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4276 5037 6065 1540
Size: 275 186 2275 761 1028 2301
Fragments arranged by size:
2301 2275 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3108 3291 3602 4887 4972 6043 3108
Size: 183 311 1285 85 1071 3891
Fragments arranged by size:
3891 1285 1071 311 183 85
BsmI GAATG_Cn'
Cuts at: 2538 2947 4337 4430 2538
Size: 409 1390 93 4934
Fragments arranged by size:
4934 1390 409 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3578 3578
Size: 6826
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2818 3108 3257 3291 3549 3602
Size: 399 523 505 290 149 34 258 53
Cuts at: 3602 3662 3947 4145 4887 4972 6043 1391
Size: 60 285 198 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 523 505 399 290 285
258 198 149 85 60 53 34
BspEI T'CCGG_A
Cuts at: 6489 6489
Size: 6826
BspHI T'CATG_A
Cuts at: 4715 6445 4715
Size: 1730 5096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2400 2953 3702 3764 4181 844
Size: 1556 553 749 62 417 3489
Fragments arranged by size:
3489 1556 749 553 417 62
BsrBI CCG'CTC
Cuts at: 1394 1823 2154 3511 4713 1394
Size: 429 331 1357 1202 3507
Fragments arranged by size:
3507 1357 1202 429 331
BsrDI GCAATG_nn'
Cuts at: 65 1248 2518 2904 3493 5321 5495 65
Size: 1183 1270 386 589 1828 174 1396
Fragments arranged by size:
1828 1396 1270 1183 589 386 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2427 2792 3832 5470 5657 1700
Size: 110 617 365 1040 1638 187 2869
Fragments arranged by size:
2869 1638 1040 617 365 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6826
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6826
BssSI C'ACGA_G
Cuts at: 4886 5898 4886
Size: 1012 5814
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6826
BstBI TT'CG_AA
Cuts at: 5630 5630
Size: 6826
BstEII G'GTnAC_C
Cuts at: 1184 2387 3133 1184
Size: 1203 746 4877
Fragments arranged by size:
4877 1203 746
BstXI CCAn_nnnn'nTGG
Cuts at: 2254 2306 3748 4059 2254
Size: 52 1442 311 5021
Fragments arranged by size:
5021 1442 311 52
BstYI r'GATC_y
Cuts at: 1972 2121 2257 2802 3438 4156 4523 4908 4925
Size: 149 136 545 636 718 367 385 17
Cuts at: 4925 6377 6463 6475 6821 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 718 636 545 385 367 346
149 136 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2563 2574 2563
Size: 11 6815
BtgI C'CryG_G
Cuts at: 513 1065 2004 2272 3388 3612 3747 3937 3976
Size: 552 939 268 1116 224 135 190 39
Cuts at: 3976 4058 513
Size: 82 3281
Fragments arranged by size:
3281 1116 939 552 268 224 190 135
82 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6087
Fragments arranged by size:
6087 683 56
BtsI GCAGTG_nn'
Cuts at: 3484 4342 5136 5156 3484
Size: 858 794 20 5154
Fragments arranged by size:
5154 858 794 20
ClaI AT'CG_AT
Cuts at: 1554 2356 1554
Size: 802 6024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6791
DraI TTT'AAA
Cuts at: 4130 4130
Size: 6826
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2213 3095 5833 809
Size: 507 897 882 2738 1802
Fragments arranged by size:
2738 1802 897 882 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2465 3703 3723
Size: 54 1425 50 275 18 635 1238 20
Cuts at: 3723 3760 3931 4040 4273 5162 6791 8
Size: 37 171 109 233 889 1629 43
Fragments arranged by size:
1629 1425 1238 889 635 275 233 171
109 54 50 43 37 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 4273 1537
Size: 275 2461 4090
Fragments arranged by size:
4090 2461 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2384 3275 3569 4756 1353
Size: 1031 891 294 1187 3423
Fragments arranged by size:
3423 1187 1031 891 294
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2437 2840 5413 5787 5933 387
Size: 1064 138 848 403 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 848 403 374 146 138
EcoICRI GAG'CTC
Cuts at: 3106 3106
Size: 6826
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 3470 3668 3825 3895 4051 4889
Size: 393 493 1345 198 157 70 156 838
Cuts at: 4889 5486 6273 6541 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1345 838 787 597 493 393 268
198 157 156 70
EcoNI CCTnn'n_nnAGG
Cuts at: 3761 3761
Size: 6826
EcoO109I rG'GnC_Cy
Cuts at: 2035 2048 2206 2444 3115 3260 3733 3766 2035
Size: 13 158 238 671 145 473 33 5095
Fragments arranged by size:
5095 671 473 238 158 145 33 13
EcoRI G'AATT_C
Cuts at: 4135 4513 4135
Size: 378 6448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 6826
FspI TGC'GCA
Cuts at: 2243 3074 5332 2243
Size: 831 2258 3737
Fragments arranged by size:
3737 2258 831
HaeII r_GCGC'y
Cuts at: 1678 2732 3691 3976 5973 1678
Size: 1054 959 285 1997 2531
Fragments arranged by size:
2531 1997 1054 959 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2715 2747 3430 3462 5467
Size: 32 135 32 1143 32 683 32 2005
Cuts at: 5467 5499 5541 5573 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2005 1143 683 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4170 4416 669
Size: 988 281 2232 246 3079
Fragments arranged by size:
3079 2232 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6826
HpaI GTT'AAC
Cuts at: 4416 4416
Size: 6826
KasI G'GCGC_C
Cuts at: 3972 3972
Size: 6826
KpnI G_GTAC'C
Cuts at: 4151 4151
Size: 6826
MfeI C'AATT_G
Cuts at: 4425 4425
Size: 6826
MluI A'CGCG_T
Cuts at: 6702 6702
Size: 6826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2235 2331 2503 3300 4060 5940 6124 1305
Size: 930 96 172 797 760 1880 184 2007
Fragments arranged by size:
2007 1880 930 797 760 184 172 96
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3705 3725 3933 10
Size: 54 1425 343 1873 20 208 2903
Fragments arranged by size:
2903 1873 1425 343 208 54 20
MslI CAynn'nnrTG
Cuts at: 518 1166 2073 2933 3746 4784 5143 5302 518
Size: 648 907 860 813 1038 359 159 2042
Fragments arranged by size:
2042 1038 907 860 813 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2683 3122 3390 3592 3808 4071 4242
Size: 409 668 439 268 202 216 263 171
Cuts at: 4242 4919 6067 6312 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 668 439 409 268 263
245 216 202 171
NaeI GCC'GGC
Cuts at: 3834 3834
Size: 6826
NarI GG'CG_CC
Cuts at: 3973 3973
Size: 6826
NcoI C'CATG_G
Cuts at: 513 1065 2004 3747 513
Size: 552 939 1743 3592
Fragments arranged by size:
3592 1743 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6826
NgoMIV G'CCGG_C
Cuts at: 3832 3832
Size: 6826
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6826
NotI GC'GGCC_GC
Cuts at: 4273 4273
Size: 6826
NspI r_CATG'y
Cuts at: 1132 1229 4184 5729 1132
Size: 97 2955 1545 2229
Fragments arranged by size:
2955 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2262 1348
Size: 914 5912
PciI A'CATG_T
Cuts at: 5725 5725
Size: 6826
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2958 3541 4012 1108
Size: 1850 583 471 3922
Fragments arranged by size:
3922 1850 583 471
PfoI T'CCnGG_A
Cuts at: 2967 2967
Size: 6826
PmeI GTTT'AAAC
Cuts at: 4130 4130
Size: 6826
PmlI CAC'GTG
Cuts at: 3361 3361
Size: 6826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 4895 4927 6408 6440 2867
Size: 32 1996 32 1481 32 3253
Fragments arranged by size:
3253 1996 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2035 2206 3115 3733 2035
Size: 171 909 618 5128
Fragments arranged by size:
5128 909 618 171
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6826
PsiI TTA'TAA
Cuts at: 4396 4396
Size: 6826
PstI C_TGCA'G
Cuts at: 830 2026 2337 2823 3479 3775 4178 830
Size: 1196 311 486 656 296 403 3478
Fragments arranged by size:
3478 1196 656 486 403 311 296
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6826
PvuII CAG'CTG
Cuts at: 2683 3808 2683
Size: 1125 5701
SacI G_AGCT'C
Cuts at: 3108 3108
Size: 6826
SacII CC_GC'GG
Cuts at: 3391 3391
Size: 6826
SalI G'TCGA_C
Cuts at: 1936 4168 1936
Size: 2232 4594
SanDI GG'GwC_CC
Cuts at: 2035 2206 3115 2035
Size: 171 909 5746
Fragments arranged by size:
5746 909 171
SapI GCTCTTCn'nnn_
Cuts at: 3275 3569 3275
Size: 294 6532
SbfI CC_TGCA'GG
Cuts at: 3775 4178 3775
Size: 403 6423
ScaI AGT'ACT
Cuts at: 5074 6786 5074
Size: 1712 5114
SfcI C'TryA_G
Cuts at: 826 1045 2022 2211 2333 2653 2819 3475 3771
Size: 219 977 189 122 320 166 656 296
Cuts at: 3771 4174 5309 5990 6181 826
Size: 403 1135 681 191 1471
Fragments arranged by size:
1471 1135 977 681 656 403 320 296
219 191 189 166 122
SfoI GGC'GCC
Cuts at: 3974 3974
Size: 6826
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6826
SmaI CCC'GGG
Cuts at: 1872 2056 2741 4153 1872
Size: 184 685 1412 4545
Fragments arranged by size:
4545 1412 685 184
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4930 5831 6093 820
Size: 197 926 2987 901 262 1553
Fragments arranged by size:
2987 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4617 493
Size: 4124 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6826
SphI G_CATG'C
Cuts at: 1132 4184 1132
Size: 3052 3774
SspI AAT'ATT
Cuts at: 5 52 4750 5
Size: 47 4698 2081
Fragments arranged by size:
4698 2081 47
StuI AGG'CCT
Cuts at: 1517 2879 1517
Size: 1362 5464
StyI C'CwwG_G
Cuts at: 513 1065 2004 2506 3747 4265 513
Size: 552 939 502 1241 518 3074
Fragments arranged by size:
3074 1241 939 552 518 502
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2750 3419 3640 4060 4853 4870 5023
Size: 1255 1170 669 221 420 793 17 153
Cuts at: 5023 5208 5653 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1170 793 669 445 420 221
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5072 6784 96
Size: 274 80 33 51 1443 3095 1712 138
Fragments arranged by size:
3095 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2391 2423 3893 3925 6786 6818 2391
Size: 32 1470 32 2861 32 2399
Fragments arranged by size:
2861 2399 1470 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3370 1316
Size: 2054 4772
XbaI T'CTAG_A
Cuts at: 4162 4162
Size: 6826
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2701 2722 3046 3718 2701
Size: 21 324 672 5809
Fragments arranged by size:
5809 672 324 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6826
XmaI C'CCGG_G
Cuts at: 1870 2054 2739 4151 1870
Size: 184 685 1412 4545
Fragments arranged by size:
4545 1412 685 184
XmnI GAAnn'nnTTC
Cuts at: 2064 4955 2064
Size: 2891 3935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4634 276
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SexAI SfiI SrfI SwaI