(Circular) (Six-base) MAPSORT of: pFN21AB5830.seq Check: 4041 from: 1 to: 6148 pFN21AB5830 6148 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2820 3306 3491 1937 Size: 883 486 185 4594 Fragments arranged by size: 4594 883 486 185 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2111 2669 4211 5595 5863 1632 Size: 479 558 1542 1384 268 1917 Fragments arranged by size: 1917 1542 1384 558 479 268 AflII C'TTAA_G Cuts at: 820 1017 3158 820 Size: 197 2141 3810 Fragments arranged by size: 3810 2141 197 AflIII A'CryG_T Cuts at: 1203 5047 6024 1203 Size: 3844 977 1327 Fragments arranged by size: 3844 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 2046 1166 Size: 880 5268 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3209 3241 3209 Size: 32 6116 AlwNI CAG_nnn'CTG Cuts at: 3891 5463 3891 Size: 1572 4576 ApaI G_GGCC'C Cuts at: 2347 2761 3195 2347 Size: 414 434 5300 Fragments arranged by size: 5300 434 414 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 3329 3457 3683 3835 5944 1493 Size: 1836 128 226 152 2109 1697 Fragments arranged by size: 2109 1836 1697 226 152 128 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 2129 2730 3128 3473 1870 Size: 73 186 601 398 345 4545 Fragments arranged by size: 4545 601 398 345 186 73 BamHI G'GATC_C Cuts at: 3478 3845 3478 Size: 367 5781 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2628 2956 3469 4924 618 Size: 325 217 627 841 328 513 1455 1842 Fragments arranged by size: 1842 1455 841 627 513 328 325 217 BanII G_rGCy'C Cuts at: 1391 2347 2761 3087 3195 3467 1391 Size: 956 414 326 108 272 4072 Fragments arranged by size: 4072 956 414 326 272 108 BbeI G_GCGC'C Cuts at: 2960 2960 Size: 6148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2201 2734 928 Size: 971 302 533 4342 Fragments arranged by size: 4342 971 533 302 BbvCI CC'TCA_GC Cuts at: 3233 3233 Size: 6148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 1903 Size: 34 2402 34 3678 Fragments arranged by size: 3678 2402 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4040 5250 4040 Size: 1210 4938 BclI T'GATC_A Cuts at: 1548 2140 1548 Size: 592 5556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4759 136 Size: 107 122 71 631 605 3087 1525 Fragments arranged by size: 3087 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6143 6143 Size: 6148 BlpI GC'TnA_GC Cuts at: 3122 3565 3122 Size: 443 5705 Bme1580I G_kGCm'C Cuts at: 1790 2347 2761 3195 4209 5365 1790 Size: 557 414 434 1014 1156 2573 Fragments arranged by size: 2573 1156 1014 557 434 414 BmgBI CAC'GTC Cuts at: 1318 2815 3253 3297 1318 Size: 1497 438 44 4169 Fragments arranged by size: 4169 1497 438 44 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2379 3334 3412 Size: 416 53 83 399 24 962 955 78 Cuts at: 3412 3879 4837 442 Size: 467 958 1753 Fragments arranged by size: 1753 962 958 955 467 416 399 83 78 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2800 2800 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2089 2265 2600 2990 3410 4808 1239 Size: 850 176 335 390 420 1398 2579 Fragments arranged by size: 2579 1398 850 420 390 335 176 Bpu10I CC'TnA_GC Cuts at: 3233 3233 Size: 6148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2318 2813 4273 5138 5436 2318 Size: 495 1460 865 298 3030 Fragments arranged by size: 3030 1460 865 495 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4811 882 Size: 617 3312 2219 Fragments arranged by size: 3312 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2272 3939 493 Size: 711 1068 1667 2702 Fragments arranged by size: 2702 1667 1068 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2957 3955 4337 4972 Size: 53 83 186 936 1424 998 382 635 Cuts at: 4972 275 Size: 1451 Fragments arranged by size: 1451 1424 998 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4581 4979 5253 5400 5811 4581 Size: 398 274 147 411 4918 Fragments arranged by size: 4918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2153 2183 3209 3239 1178 Size: 30 165 30 750 30 1026 30 4087 Fragments arranged by size: 4087 1026 750 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2309 2383 2420 2765 1186 Size: 1123 74 37 345 4569 Fragments arranged by size: 4569 1123 345 74 37 BseYI C'CCAG_C Cuts at: 3010 3149 3194 5351 5847 3010 Size: 139 45 2157 496 3311 Fragments arranged by size: 3311 2157 496 139 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2793 1163 Size: 573 1057 4518 Fragments arranged by size: 4518 1057 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2674 3043 3598 4359 5387 1540 Size: 275 186 673 369 555 761 1028 2301 Fragments arranged by size: 2301 1028 761 673 555 369 275 186 BsiHKAI G_wGCw'C Cuts at: 3087 3222 4209 4294 5365 3087 Size: 135 987 85 1071 3870 Fragments arranged by size: 3870 1071 987 135 85 BsmI GAATG_Cn' Cuts at: 2186 3659 3752 2186 Size: 1473 93 4582 Fragments arranged by size: 4582 1473 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2347 2761 3087 3195 3222 3467 4209 Size: 399 557 414 326 108 27 245 742 Cuts at: 4209 4294 5365 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 557 414 399 326 245 108 85 27 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 4037 5767 4037 Size: 1730 4418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3503 844 Size: 2659 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2755 4035 1394 Size: 429 932 1280 3507 Fragments arranged by size: 3507 1280 932 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2568 4643 4817 65 Size: 1183 1320 2075 174 1396 Fragments arranged by size: 2075 1396 1320 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4792 4979 1700 Size: 110 2982 187 2869 Fragments arranged by size: 2982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 3068 4208 5220 3068 Size: 1140 1012 3996 Fragments arranged by size: 3996 1140 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6148 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6148 BstXI CCAn_nnnn'nTGG Cuts at: 2048 2317 2048 Size: 269 5879 BstYI r'GATC_y Cuts at: 1972 2547 3102 3478 3845 4230 4247 5699 5785 Size: 575 555 376 367 385 17 1452 86 Cuts at: 5785 5797 6143 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 575 555 385 376 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2821 2821 Size: 6148 Bsu36I CC'TnA_GG Cuts at: 2961 3288 2961 Size: 327 5821 BtgI C'CryG_G Cuts at: 513 1065 2075 2361 2994 513 Size: 552 1010 286 633 3667 Fragments arranged by size: 3667 1010 633 552 286 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3213 487 Size: 683 56 1987 3422 Fragments arranged by size: 3422 1987 683 56 BtsI GCAGTG_nn' Cuts at: 2246 2640 3664 4458 4478 2246 Size: 394 1024 794 20 3916 Fragments arranged by size: 3916 1024 794 394 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5155 809 Size: 507 3839 1802 Fragments arranged by size: 3839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2527 2863 2986 Size: 54 1425 50 275 18 697 336 123 Cuts at: 2986 3040 3432 3595 4484 6113 8 Size: 54 392 163 889 1629 43 Fragments arranged by size: 1629 1425 889 697 392 336 275 163 123 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3040 3595 1537 Size: 275 1228 555 4090 Fragments arranged by size: 4090 1228 555 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 3083 3093 4078 1353 Size: 734 996 10 985 3423 Fragments arranged by size: 3423 996 985 734 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3085 3085 Size: 6148 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2089 2111 2265 2600 2669 2990 3410 Size: 393 457 22 154 335 69 321 420 Cuts at: 3410 4211 4808 5595 5863 1239 Size: 801 597 787 268 1524 Fragments arranged by size: 1524 801 787 597 457 420 393 335 321 268 154 69 22 EcoNI CCTnn'n_nnAGG Cuts at: 2480 2555 2480 Size: 75 6073 EcoO109I rG'GnC_Cy Cuts at: 2134 2262 2344 2356 2611 2940 3166 3191 2134 Size: 128 82 12 255 329 226 25 5091 Fragments arranged by size: 5091 329 255 226 128 82 25 12 EcoRI G'AATT_C Cuts at: 3457 3835 3457 Size: 378 5770 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2506 2674 2506 Size: 168 5980 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 2960 3076 5295 1678 Size: 1282 116 2219 2531 Fragments arranged by size: 2531 2219 1282 116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3122 3154 4789 4821 4863 Size: 32 135 32 1550 32 1635 32 42 Cuts at: 4863 4895 1373 Size: 32 2626 Fragments arranged by size: 2626 1635 1550 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3307 3492 3738 669 Size: 988 281 1369 185 246 3079 Fragments arranged by size: 3079 1369 988 281 246 185 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KasI G'GCGC_C Cuts at: 2956 2956 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5262 5446 1305 Size: 3957 184 2007 Fragments arranged by size: 3957 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2865 10 Size: 54 1425 343 1033 3293 Fragments arranged by size: 3293 1425 1033 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2046 2315 2507 3092 4106 4465 4624 Size: 648 880 269 192 585 1014 359 159 Cuts at: 4624 518 Size: 2042 Fragments arranged by size: 2042 1014 880 648 585 359 269 192 159 MspA1I CmG'CkG Cuts at: 1606 2188 2341 3403 3564 4241 5389 5634 1606 Size: 582 153 1062 161 677 1148 245 2120 Fragments arranged by size: 2120 1148 1062 677 582 245 161 153 NarI GG'CG_CC Cuts at: 2957 2957 Size: 6148 NcoI C'CATG_G Cuts at: 513 1065 2361 513 Size: 552 1296 4300 Fragments arranged by size: 4300 1296 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3040 3595 3040 Size: 555 5593 NspI r_CATG'y Cuts at: 1132 1229 3506 5051 1132 Size: 97 2277 1545 2229 Fragments arranged by size: 2277 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PmlI CAC'GTG Cuts at: 2272 2272 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3209 3241 4217 4249 5730 5762 3209 Size: 32 976 32 1481 32 3595 Fragments arranged by size: 3595 1481 976 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2262 2356 2611 2940 3166 2262 Size: 94 255 329 226 5244 Fragments arranged by size: 5244 329 255 226 94 PshAI GACnn'nnGTC Cuts at: 1613 2249 1613 Size: 636 5512 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PspOMI G'GGCC_C Cuts at: 2343 2757 3191 2343 Size: 414 434 5300 Fragments arranged by size: 5300 434 414 PstI C_TGCA'G Cuts at: 830 2241 3500 830 Size: 1411 1259 3478 Fragments arranged by size: 3478 1411 1259 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2188 2188 Size: 6148 SacI G_AGCT'C Cuts at: 3087 3087 Size: 6148 SalI G'TCGA_C Cuts at: 1936 3305 3490 1936 Size: 1369 185 4594 Fragments arranged by size: 4594 1369 185 SanDI GG'GwC_CC Cuts at: 2940 2940 Size: 6148 SapI GCTCTTCn'nnn_ Cuts at: 2087 2087 Size: 6148 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 2798 4396 6108 2798 Size: 1598 1712 2838 Fragments arranged by size: 2838 1712 1598 SfcI C'TryA_G Cuts at: 826 1045 2237 3496 4631 5312 5503 826 Size: 219 1192 1259 1135 681 191 1471 Fragments arranged by size: 1471 1259 1192 1135 681 219 191 SfoI GGC'GCC Cuts at: 2958 2958 Size: 6148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 2732 3475 1872 Size: 860 743 4545 Fragments arranged by size: 4545 860 743 SmlI C'TyrA_G Cuts at: 820 1017 1943 2333 2792 3158 4252 5153 5415 Size: 197 926 390 459 366 1094 901 262 Cuts at: 5415 820 Size: 1553 Fragments arranged by size: 1553 1094 926 901 459 390 366 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 4072 5 Size: 47 4020 2081 Fragments arranged by size: 4020 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6148 StyI C'CwwG_G Cuts at: 513 1065 2361 2476 2574 3204 3587 513 Size: 552 1296 115 98 630 383 3074 Fragments arranged by size: 3074 1296 630 552 383 115 98 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 3002 4175 4192 4345 4530 4975 Size: 1255 633 789 1173 17 153 185 445 Cuts at: 4975 325 Size: 1498 Fragments arranged by size: 1498 1255 1173 789 633 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2796 4394 6106 Size: 274 80 33 51 1443 819 1598 1712 Cuts at: 6106 96 Size: 138 Fragments arranged by size: 1712 1598 1443 819 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2067 2099 2357 2389 6108 6140 2067 Size: 32 258 32 3719 32 2075 Fragments arranged by size: 3719 2075 258 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3254 1316 Size: 1938 4210 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3211 3211 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 2730 3473 1870 Size: 860 743 4545 Fragments arranged by size: 4545 860 743 XmnI GAAnn'nnTTC Cuts at: 2456 2930 4277 2456 Size: 474 1347 4327 Fragments arranged by size: 4327 1347 474 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI