(Circular) (Six-base) MAPSORT of: pFN21AE1692.seq Check: 2191 from: 1 to: 5305 pFN21AE1692 5305 bp With 182 enzymes: SgfI * March 28, 2012 12:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2343 3115 278 Size: 53 83 186 936 807 772 2468 Fragments arranged by size: 2468 936 807 772 186 83 53 AccI GT'mk_AC Cuts at: 1937 2648 1937 Size: 711 4594 Acc65I G'GTAC_C Cuts at: 2626 2626 Size: 5305 AclI AA'CG_TT Cuts at: 1563 3432 3805 1563 Size: 1869 373 3063 Fragments arranged by size: 3063 1869 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2321 3368 4752 5020 1632 Size: 689 1047 1384 268 1917 Fragments arranged by size: 1917 1384 1047 689 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5108 AflIII A'CryG_T Cuts at: 1203 4204 5181 1203 Size: 3001 977 1327 Fragments arranged by size: 3001 1327 977 AgeI A'CCGG_T Cuts at: 4136 4136 Size: 5305 AhdI GACnn_n'nnGTC Cuts at: 4034 4034 Size: 5305 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5305 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5305 AlwNI CAG_nnn'CTG Cuts at: 2066 3048 4620 2066 Size: 982 1572 2751 Fragments arranged by size: 2751 1572 982 ApaI G_GGCC'C Cuts at: 2424 2424 Size: 5305 ApaLI G'TGCA_C Cuts at: 3362 4518 3362 Size: 1156 4149 ApoI r'AATT_y Cuts at: 1493 2614 2840 2992 5101 1493 Size: 1121 226 152 2109 1697 Fragments arranged by size: 2109 1697 1121 226 152 AseI AT'TA_AT Cuts at: 160 1032 3859 160 Size: 872 2827 1606 Fragments arranged by size: 2827 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5305 AvaI C'yCGr_G Cuts at: 1870 1943 2225 2430 2630 1870 Size: 73 282 205 200 4545 Fragments arranged by size: 4545 282 205 200 73 BamHI G'GATC_C Cuts at: 2635 3002 2635 Size: 367 4938 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2626 4081 618 Size: 325 217 627 839 1455 1842 Fragments arranged by size: 1842 1455 839 627 325 217 BanII G_rGCy'C Cuts at: 1391 2424 2624 1391 Size: 1033 200 4072 Fragments arranged by size: 4072 1033 200 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2212 928 Size: 971 313 4021 Fragments arranged by size: 4021 971 313 BbvCI CC'TCA_GC Cuts at: 2406 2406 Size: 5305 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3496 3530 1903 Size: 34 1559 34 3678 Fragments arranged by size: 3678 1559 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2408 3197 4407 2408 Size: 789 1210 3306 Fragments arranged by size: 3306 1210 789 BclI T'GATC_A Cuts at: 1548 1548 Size: 5305 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2112 2279 3916 Size: 107 122 71 631 605 440 167 1637 Cuts at: 3916 136 Size: 1525 Fragments arranged by size: 1637 1525 631 605 440 167 122 107 71 BglII A'GATC_T Cuts at: 5300 5300 Size: 5305 BlpI GC'TnA_GC Cuts at: 2283 2722 2283 Size: 439 4866 Bme1580I G_kGCm'C Cuts at: 1790 2424 3366 4522 1790 Size: 634 942 1156 2573 Fragments arranged by size: 2573 1156 942 634 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5305 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2103 3036 3994 Size: 416 53 83 399 24 686 933 958 Cuts at: 3994 442 Size: 1753 Fragments arranged by size: 1753 958 933 686 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2147 1056 Size: 1091 4214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2221 2234 2330 2354 2372 2447 2519 3965 Size: 982 13 96 24 18 75 72 1446 Cuts at: 3965 1239 Size: 2579 Fragments arranged by size: 2579 1446 982 96 75 72 24 18 13 Bpu10I CC'TnA_GC Cuts at: 2406 2406 Size: 5305 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2171 3430 4295 4593 2171 Size: 1259 865 298 2883 Fragments arranged by size: 2883 1259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2535 3968 882 Size: 617 1036 1433 2219 Fragments arranged by size: 2219 1433 1036 617 BsaAI yAC'GTr Cuts at: 493 1204 3096 493 Size: 711 1892 2702 Fragments arranged by size: 2702 1892 711 BsaBI GATnn'nnATC Cuts at: 5299 5299 Size: 5305 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 2340 3112 3494 Size: 53 83 186 936 636 171 772 382 Cuts at: 3494 4129 275 Size: 635 1451 Fragments arranged by size: 1451 936 772 636 635 382 186 171 83 53 BsaWI w'CCGG_w Cuts at: 3738 4136 4410 4557 4968 3738 Size: 398 274 147 411 4075 Fragments arranged by size: 4075 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 1178 Size: 30 165 30 960 30 4090 Fragments arranged by size: 4090 960 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2522 1186 Size: 1336 3969 BseYI C'CCAG_C Cuts at: 2326 2382 4508 5004 2326 Size: 56 2126 496 2627 Fragments arranged by size: 2627 2126 496 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2351 2438 1163 Size: 573 615 87 4030 Fragments arranged by size: 4030 615 573 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2755 3516 4544 1540 Size: 275 186 754 761 1028 2301 Fragments arranged by size: 2301 1028 761 754 275 186 BsiHKAI G_wGCw'C Cuts at: 3366 3451 4522 3366 Size: 85 1071 4149 Fragments arranged by size: 4149 1071 85 BsmI GAATG_Cn' Cuts at: 2816 2909 2816 Size: 93 5212 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2424 2624 3366 3451 4522 1391 Size: 399 634 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 634 399 200 85 BspEI T'CCGG_A Cuts at: 4968 4968 Size: 5305 BspHI T'CATG_A Cuts at: 3194 4924 3194 Size: 1730 3575 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2106 2660 844 Size: 1262 554 3489 Fragments arranged by size: 3489 1262 554 BsrBI CCG'CTC Cuts at: 1394 1823 3192 1394 Size: 429 1369 3507 Fragments arranged by size: 3507 1369 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3800 3974 65 Size: 1183 2552 174 1396 Fragments arranged by size: 2552 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2457 3949 4136 1700 Size: 110 647 1492 187 2869 Fragments arranged by size: 2869 1492 647 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5305 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5305 BssSI C'ACGA_G Cuts at: 3365 4377 3365 Size: 1012 4293 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5305 BstBI TT'CG_AA Cuts at: 4109 4109 Size: 5305 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5305 BstYI r'GATC_y Cuts at: 1972 2635 3002 3387 3404 4856 4942 4954 5300 Size: 663 367 385 17 1452 86 12 346 Cuts at: 5300 1972 Size: 1977 Fragments arranged by size: 1977 1452 663 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2240 2240 Size: 5305 BtgI C'CryG_G Cuts at: 513 1065 2004 2022 2055 513 Size: 552 939 18 33 3763 Fragments arranged by size: 3763 939 552 33 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4566 Fragments arranged by size: 4566 683 56 BtsI GCAGTG_nn' Cuts at: 2821 3615 3635 2821 Size: 794 20 4491 Fragments arranged by size: 4491 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5305 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5270 DraI TTT'AAA Cuts at: 2609 2609 Size: 5305 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4312 809 Size: 507 2996 1802 Fragments arranged by size: 2996 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2556 2752 Size: 54 1425 50 275 18 222 504 196 Cuts at: 2752 3641 5270 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 504 275 222 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2752 1537 Size: 275 940 4090 Fragments arranged by size: 4090 940 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2528 3235 1353 Size: 819 356 707 3423 Fragments arranged by size: 3423 819 707 356 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2516 3892 4266 4412 387 Size: 1064 138 927 1376 374 146 1280 Fragments arranged by size: 1376 1280 1064 927 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2221 2234 2321 2330 2354 2372 2447 Size: 393 589 13 87 9 24 18 75 Cuts at: 2447 2519 3368 3965 4752 5020 1239 Size: 72 849 597 787 268 1524 Fragments arranged by size: 1524 849 787 597 589 393 268 87 75 72 24 18 13 9 EcoO109I rG'GnC_Cy Cuts at: 2063 2236 2305 2420 2427 2470 2063 Size: 173 69 115 7 43 4898 Fragments arranged by size: 4898 173 115 69 43 7 EcoRI G'AATT_C Cuts at: 2614 2992 2614 Size: 378 4927 FspI TGC'GCA Cuts at: 3811 3811 Size: 5305 HaeII r_GCGC'y Cuts at: 1678 2266 4452 1678 Size: 588 2186 2531 Fragments arranged by size: 2531 2186 588 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3946 3978 4020 4052 1373 Size: 32 135 32 2374 32 42 32 2626 Fragments arranged by size: 2626 2374 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2586 2649 2895 669 Size: 988 281 648 63 246 3079 Fragments arranged by size: 3079 988 648 281 246 63 HindIII A'AGCT_T Cuts at: 748 748 Size: 5305 HpaI GTT'AAC Cuts at: 2895 2895 Size: 5305 KpnI G_GTAC'C Cuts at: 2630 2630 Size: 5305 MfeI C'AATT_G Cuts at: 2904 2904 Size: 5305 MluI A'CGCG_T Cuts at: 5181 5181 Size: 5305 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4419 4603 1305 Size: 3114 184 2007 Fragments arranged by size: 3114 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3483 Fragments arranged by size: 3483 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3263 3622 3781 518 Size: 648 2097 359 159 2042 Fragments arranged by size: 2097 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2318 2489 2721 3398 4546 4791 1606 Size: 712 171 232 677 1148 245 2120 Fragments arranged by size: 2120 1148 712 677 245 232 171 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3814 Fragments arranged by size: 3814 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5305 NheI G'CTAG_C Cuts at: 1052 2143 1052 Size: 1091 4214 NotI GC'GGCC_GC Cuts at: 2752 2752 Size: 5305 NspI r_CATG'y Cuts at: 1132 1229 2663 4208 1132 Size: 97 1434 1545 2229 Fragments arranged by size: 2229 1545 1434 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5305 PciI A'CATG_T Cuts at: 4204 4204 Size: 5305 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5305 PfoI T'CCnGG_A Cuts at: 2307 2463 2307 Size: 156 5149 PmeI GTTT'AAAC Cuts at: 2609 2609 Size: 5305 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3374 3406 4887 4919 3374 Size: 32 1481 32 3760 Fragments arranged by size: 3760 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2236 2305 2236 Size: 69 5236 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5305 PsiI TTA'TAA Cuts at: 2875 2875 Size: 5305 PspOMI G'GGCC_C Cuts at: 2420 2420 Size: 5305 PstI C_TGCA'G Cuts at: 830 2014 2657 830 Size: 1184 643 3478 Fragments arranged by size: 3478 1184 643 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5305 SalI G'TCGA_C Cuts at: 1936 2647 1936 Size: 711 4594 SbfI CC_TGCA'GG Cuts at: 2657 2657 Size: 5305 ScaI AGT'ACT Cuts at: 3553 5265 3553 Size: 1712 3593 SfcI C'TryA_G Cuts at: 826 1045 2010 2653 3788 4469 4660 826 Size: 219 965 643 1135 681 191 1471 Fragments arranged by size: 1471 1135 965 681 643 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5305 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5305 SmaI CCC'GGG Cuts at: 1872 2227 2432 2632 1872 Size: 355 205 200 4545 Fragments arranged by size: 4545 355 205 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 3409 4310 4572 820 Size: 197 926 243 1223 901 262 1553 Fragments arranged by size: 1553 1223 926 901 262 243 197 SnaBI TAC'GTA Cuts at: 493 3096 493 Size: 2603 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5305 SphI G_CATG'C Cuts at: 1132 2663 1132 Size: 1531 3774 SspI AAT'ATT Cuts at: 5 52 3229 5 Size: 47 3177 2081 Fragments arranged by size: 3177 2081 47 StuI AGG'CCT Cuts at: 1517 2393 1517 Size: 876 4429 StyI C'CwwG_G Cuts at: 513 1065 2004 2172 2274 2744 513 Size: 552 939 168 102 470 3074 Fragments arranged by size: 3074 939 552 470 168 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3332 3349 3502 3687 4132 325 Size: 1255 1752 17 153 185 445 1498 Fragments arranged by size: 1752 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3551 5263 96 Size: 274 80 33 51 1443 1574 1712 138 Fragments arranged by size: 1712 1574 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2363 2395 2582 2614 5265 5297 2363 Size: 32 187 32 2651 32 2371 Fragments arranged by size: 2651 2371 187 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5305 XbaI T'CTAG_A Cuts at: 2641 2641 Size: 5305 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2170 2170 Size: 5305 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5305 XmaI C'CCGG_G Cuts at: 1870 2225 2430 2630 1870 Size: 355 205 200 4545 Fragments arranged by size: 4545 355 205 200 XmnI GAAnn'nnTTC Cuts at: 3434 3434 Size: 5305 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2341 3113 276 Size: 53 83 186 936 807 772 2468 Fragments arranged by size: 2468 936 807 772 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI