################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I ClaI CpoI DpnII DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TfiI Tth111I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AocI 243 CCTGAGG CC^T[ACGT]AGG ApaI 430 GGGCCC GGGCC^C BbvCI 415 CCTCAGC CCTCAGC(-5/-2) BciVI 407 GTATCC GTATCC(6/5) BlpI 286 GCTGAGC GC^T[ACGT]AGC Bpu1102I 286 GCTGAGC GC^T[ACGT]AGC BsaI 551 GAGACC GGTCTC(1/5) BseRI 541 CTCCTC GAGGAG(10/8) BspMI 255 GCAGGT ACCTGC(4/8) BsrBI 213 CCGCTC CCGCTC(-3/-3) BsrFI 467 ACCGGC [GA]^CCGG[CT] Bsu36I 243 CCTGAGG CC^T[ACGT]AGG CelII 286 GCTGAGC GC^T[ACGT]AGC CfoI 267 GCGC GCG^C Cfr10I 467 ACCGGC [GA]^CCGG[CT] DrdI 577 GACCTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 35 TGGCCG [CT]^GGCC[GA] Eco81I 243 CCTGAGG CC^T[ACGT]AGG EcoNI 155 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 266 AGCGCC [GA]GCGC^[CT] HgaI 300 GACGC GACGC(5/10) HhaI 267 GCGC GCG^C HinP1I 267 GCGC G^CGC KspI 81 CCGCGG CCGC^GG MseI 158 TTAA T^TAA MslI 15 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 15 CATG CATG^ NspI 14 GCATGC [GA]CATG^[CT] PaeR7I 508 CTCGAG C^TCGAG PflMI 230 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 250 CAGCTG CAG^CTG SacII 81 CCGCGG CCGC^GG SmaI 440 CCCGGG CCC^GGG SmlI 508 CTCGAG C^T[CT][GA]AG SphI 14 GCATGC GCATG^C TaqI 509 TCGA T^CGA Tru9I 158 TTAA T^TAA Tsp509I 559 AATT ^AATT TthHB8I 509 TCGA T^CGA Van91I 230 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 508 CTCGAG C^TCGAG XmaCI 440 CCCGGG C^CCGGG XmaI 440 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 82 CGCG CG^CG 548 CGCG AvaI 440 CCCGGG C^[CT]CG[GA]G 508 CTCGAG BanII 86 GGGCTC G[GA]GC[CT]^C 430 GGGCCC BmyI 86 GGGCTC G[AGT]GC[ACT]^C 430 GGGCCC BsgI 376 CTGCAC GTGCAG(16/14) 463 CTGCAC BsmAI 551 GAGAC GTCTC(1/5) 135 GTCTC BsoBI 440 CCCGGG C^[CT]CG[GA]G 508 CTCGAG Bsp1286I 86 GGGCTC G[AGT]GC[ACT]^C 430 GGGCCC BstF5I 96 GGATG GGATG(2/0) 348 GGATG BstUI 82 CGCG CG^CG 548 CGCG FokI 96 GGATG GGATG(9/13) 348 GGATG HinfI 184 GAGTC G^A[ACGT]TC 493 GAGTC HphI 258 GGTGA GGTGA(8/7) 521 TCACC MaeIII 52 GTGAC ^GT[ACGT]AC 520 GTCAC MvnI 82 CGCG CG^CG 548 CGCG PleI 184 GAGTC GAGTC(4/5) 493 GAGTC PstI 148 CTGCAG CTGCA^G 253 CTGCAG SfaNI 97 GATGC GCATC(5/9) 172 GATGC SfcI 148 CTGCAG C^T[GA][CT]AG 253 CTGCAG StuI 152 AGGCCT AGG^CCT 512 AGGCCT Tsp45I 52 GTGAC ^GT[GC]AC 520 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI AlwNI AvaII BbvI BcnI BpmI BsaJI BsiYI BslI BsmFI BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII DsaI EarI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HpaII ItaI Ksp632I MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIV PpuMI Sau96I ScrFI TseI TspRI